https://m2.mtmt.hu/ hun 2026-09-17 05:38 JournalArticle 35176626 VALIDATED true 0 false 2026-07-19T20:12:46.323+0000 2025-01-30T13:45:56.871+0000 2024-08-21T06:10:18.585+0000 true 10028829 Kuthi Levente /api/author/10028829 Author true 10028829 2025-01-28T10:07:30.520+0000 2025-01-28T10:07:30.520+0000 2025-01-30T13:45:56.877+0000 true 10088410 Bakosné Moráth Csilla /api/admin/10088410 Admin true Admin 10014568 /api/admin/10014568 Vándor Andrea true 10014568 true 2025-04-30T11:24:11.171+0000 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Kovács, Ákos Novel method for detecting frequent TERT promoter hot spot mutations in bladder cancer samples true true /api/journal/10000722 REVIEWED true false 10000722 false 10000722 true 1591-8890 1591-9528 Journal FOREIGN 24 1 192 192 9 2024 Telomerase reverse transcriptase promoter (TERTp) mutations are frequently targeted tumor markers, however, they reside in regions with high GC content, which poses challenges when examined with simple molecular techniques or even with next-generation sequencing (NGS). In bladder cancer (BC), TERTp mutations are particularly frequent, however, none of the available tools have demonstrated efficacy in detecting TERTp mutations via a simple noninvasive technique. Therefore, we developed a novel PCR-based method for the detection of the two most common TERTp mutations and demonstrated its use for the analysis of BC samples. The developed SHARD-PCR TERTp mutation detection technique requires PCR and restriction digestion steps that are easily implementable even in less well-equipped laboratories. Cell lines with known mutational status were utilized for method development. Matching urine and tumor tissue samples from BC patients were analyzed, and the results were validated by next-generation sequencing. Analysis of eighteen urine and corresponding tumor tissue samples by SHARD-PCR revealed perfect matches in sample pairs, which paralleled the corresponding NGS results: fourteen samples exhibited mutations at the -124 position, two samples showed mutations at the -146 position, and no mutations were detected in two samples. Our study serves as a proof-of-concept and is limited by its small sample size, nonetheless, it demonstrates that SHARD-PCR is a simple, economic and highly reliable method for detecting TERTp mutations, which are common in different cancer types. For bladder cancer, SHARD-PCR can be performed with the use of noninvasive samples and could replace or complement currently used techniques. Funding 2049202 /api/funding/2049202 false true Funding 2049203 /api/funding/2049203 false true true 2024 true true true false true false GOLD 2026-07-19 true https://doi.org/10.1007/s10238-024-01464-3 3 0 3 3 0 3 3 2 3 3 3 3 3 3 0 0 3 3 3 3 0 0 0 Folyóiratcikk Journal Article 24 3 Könyvrészlet Chapter in Book 25 0 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2025 0 2 2 2 2 2026 0 1 1 1 1 Q1 false false false false 2024-11-19T07:10:19.341+0000 Biokémiai Intézet (HRN SZBK); Biokémiai és Molekuláris Biológiai Tanszék (SZTE / TTIK / BI); Genetikai Intézet (HRN SZBK); Pathologiai Intézet (SZTE / SZAOK) 5 31 4 5 0 true 10014568 Language 10002 /api/language/10002 Angol English true 2 true PersonAuthorship 118643236 /api/authorship/118643236 1 0.2 true false false Author 10089428 /api/author/10089428 Kovács Ákos true true Kovács Ákos true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 118643237 /api/authorship/118643237 2 0.2 false false false Author 10028995 /api/author/10028995 Sükösd Farkas true 10028995 true Sükösd Farkas true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 118643238 /api/authorship/118643238 3 0.2 false false false Author 10028829 /api/author/10028829 Kuthi Levente true 10028829 true Kuthi Levente true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 118643239 /api/authorship/118643239 4 0.2 false false true Author 10000502 /api/author/10000502 Boros Imre Miklós true 10000502 true Boros Imre M true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 118643240 /api/authorship/118643240 5 0.2 false true true Author 10029430 /api/author/10029430 Vedelek Balázs true 10029430 true Vedelek Balázs true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PublicationIdentifier 26943242 /api/publicationidentifier/26943242 PlainSource 6 /api/publicationsource/6 PublicationSourceType 10001 /api/publicationsourcetype/10001 true true true DOI DOI https://doi.org/@@@ true true 6 true GOLD true IDENTICAL 10.1007/s10238-024-01464-3 https://doi.org/10.1007/s10238-024-01464-3 false true PublicationIdentifier 26943243 /api/publicationidentifier/26943243 PlainSource 1 /api/publicationsource/1 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true IDENTICAL 001290713800002 https://www.webofscience.com/wos/woscc/full-record/001290713800002 false true PublicationIdentifier 28318075 /api/publicationidentifier/28318075 SwordSource 36 /api/publicationsource/36 PublicationSourceType 10007 /api/publicationsourcetype/10007 true true true REAL REAL https://real.mtak.hu/@@@ true true 36 true GREEN true 214701 https://real.mtak.hu/214701 false true PublicationIdentifier 28109331 /api/publicationidentifier/28109331 PlainSource 3 /api/publicationsource/3 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true Scopus Scopus http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@ true true 3 true IDENTICAL 85201242903 http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85201242903 true true PublicationIdentifier 26943241 /api/publicationidentifier/26943241 PlainSource 17 /api/publicationsource/17 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true PubMed PubMed http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=@@@&dopt=Abstract true true 17 true IDENTICAL 39141194 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=39141194&dopt=Abstract false true PublicationIdentifier 28318073 /api/publicationidentifier/28318073 SwordSource 90 /api/publicationsource/90 PublicationSourceType 10007 /api/publicationsourcetype/10007 true true true SZTE Publicatio SZTE Publicatio http://publicatio.bibl.u-szeged.hu/@@@ true true 90 true GREEN true IDENTICAL 35860 http://publicatio.bibl.u-szeged.hu/35860 false true Keyword 1008662 /api/keyword/1008662 true 1008662 true Keyword 1013293 /api/keyword/1013293 true 1013293 true Keyword 1513530 /api/keyword/1513530 true true Keyword 1860029 /api/keyword/1860029 true true Keyword 2247958 /api/keyword/2247958 true true SjrRating 11443760 /api/sjrrating/11443760 80 0.24 journal RatingType 10002 /api/ratingtype/10002 sjr true true ClassificationExternal 1301 /api/classificationexternal/1301 true 1301 true Q1 DIRECT true true /api/publication/35176626 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10089428"><a href="/gui2/?type=authors&mode=browse&sel=10089428" target="_blank">Kovács Ákos (<span class="authorship-author-name">Kovács Ákos</span> <span class="authorAux-mtmt"> Molekuláris biológia</span>) </a> </span> <span class="author-affil"><span title="Szegedi Tudományegyetem">SZTE</span>/<span title="Természettudományi és Informatikai Kar">TTIK</span>/<span title="Biológia Intézet">BI</span>/Biokémiai és Molekuláris Biológiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10028995"><a href="/gui2/?type=authors&mode=browse&sel=10028995" target="_blank">Sükösd Farkas (<span class="authorship-author-name">Sükösd Farkas</span> <span class="authorAux-mtmt"> patológia</span>) </a> </span> <span class="author-affil"><span title="Szegedi Tudományegyetem">SZTE</span>/<span title="Szent-Györgyi Albert Orvostudományi Kar">SZAOK</span>/Pathologiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10028829"><a href="/gui2/?type=authors&mode=browse&sel=10028829" target="_blank">Kuthi Levente (<span class="authorship-author-name">Kuthi Levente</span> <span class="authorAux-mtmt"> Patológia</span>) </a> </span> <span class="author-affil"><span title="Szegedi Tudományegyetem">SZTE</span>/<span title="Szent-Györgyi Albert Orvostudományi Kar">SZAOK</span>/Pathologiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10000502"><a href="/gui2/?type=authors&mode=browse&sel=10000502" target="_blank">Boros Imre M ✉ (<span class="authorship-author-name">Boros Imre Miklós</span> <span class="authorAux-mtmt"> Molekuláris biológia</span>) </a> </span> <span class="author-affil"><span title="HUN-REN Szegedi Biológiai Kutatóközpont">HRN SZBK</span>/Biokémiai Intézet; <span title="Szegedi Tudományegyetem">SZTE</span>/<span title="Természettudományi és Informatikai Kar">TTIK</span>/<span title="Biológia Intézet">BI</span>/Biokémiai és Molekuláris Biológiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10029430"><a href="/gui2/?type=authors&mode=browse&sel=10029430" target="_blank">Vedelek Balázs ✉ (<span class="authorship-author-name">Vedelek Balázs</span> <span class="authorAux-mtmt"> Molekuláris biológia</span>) </a> </span> <span class="author-affil"><span title="HUN-REN Szegedi Biológiai Kutatóközpont">HRN SZBK</span>/Genetikai Intézet; <span title="Szegedi Tudományegyetem">SZTE</span>/<span title="Természettudományi és Informatikai Kar">TTIK</span>/<span title="Biológia Intézet">BI</span>/Biokémiai és Molekuláris Biológiai Tanszék</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;35176626" target="_blank">Novel method for detecting frequent TERT promoter hot spot mutations in bladder cancer samples</a></div> <div> <span class="journal-title">CLINICAL AND EXPERIMENTAL MEDICINE</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/1591-8890">1591-8890</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1591-9528">1591-9528</a>)</span>: <span class="journal-volume">24</span> <span class="journal-issue">1</span> <span class="page"> Paper 192. 9 p. </span> <span class="year">(2024)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="10.1007/s10238-024-01464-3" target="_blank" href="https://doi.org/10.1007/s10238-024-01464-3"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="001290713800002" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/001290713800002"> WoS </a> </span> <span class="id identifier oa_GREEN" title=" Green "> <a style="color:black" title="214701" target="_blank" href="https://real.mtak.hu/214701"> REAL </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="85201242903" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85201242903"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="39141194" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=39141194&dopt=Abstract"> PubMed </a> </span> <span class="id identifier oa_GREEN" title=" Green "> <a style="color:blue" title="35860" target="_blank" href="http://publicatio.bibl.u-szeged.hu/35860"> SZTE Publicatio </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Folyóirat szakterülete: Scopus - Biochemistry, Genetics and Molecular Biology (miscellaneous)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Medicine (miscellaneous)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 3</span> | Független: 3 | Függő: 0 | Nem jelölt: 0 | WoS jelölt: 2 | Scopus jelölt:&nbsp;3 | WoS/Scopus jelölt:&nbsp;3 | DOI jelölt:&nbsp;3 </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 35176626</span> | <span class="status-data status-VALIDATED"> Egyeztetett </span> Forrás | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">kézi felvitel</span> </div> <div class="funder"> (GINOP-2.3.2-15- 2016-00020), (2022-2.1.1-NL-2022-00008) </div> <div class="lastModified">Utolsó módosítás: 2025.01.30. 14:45 Bakosné Moráth Csilla (SZTE admin5 TTIK Biokémiai és Molekuláris Biológiai Tanszék) </div> <div class="lockedBy">Központi kezelésű 2025.04.30. 13:24 Vándor Andrea (VA, admin) </div> </div></div>