https://m2.mtmt.hu/ hun 2026-09-23 05:34 JournalArticle 35069431 VALIDATED true 0 false 2026-05-23T23:18:42.356+0000 2025-02-12T14:07:14.873+0000 2024-06-25T08:49:38.303+0000 true 10039043 Milánkovics Róbert /api/admin/10039043 Admin true 10039043 2025-02-06T09:44:31.978+0000 2025-02-06T09:44:31.978+0000 2025-02-06T10:17:52.472+0000 true 10090847 Horváth Viktória /api/admin/10090847 Admin true 2025-02-12T14:07:15.089+0000 true 10086190 Bara Eszter /api/admin/10086190 Admin true Admin 10025544 /api/admin/10025544 Lévayné Deseő Katalin true 10025544 true 2025-06-04T23:43:20.924+0000 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Ishiko, Shinya Liposomal nanocarriers of preassembled glycocalyx restore normal venular permeability and shear stress sensitivity in sepsis: assessed quantitatively with a novel microchamber system true true /api/journal/21227 REVIEWED true false 21227 false 21227 true 0363-6135 1522-1539 Journal FOREIGN 327 2 H390 H398 H390 9 2024 The endothelial glycocalyx (EG), covering the luminal side of endothelial cells, regulates vascular permeability and senses wall shear stress. In sepsis, EG undergoes degradation leading to increased permeability and edema formation. We hypothesized that restoring EG integrity using liposomal nanocarriers of preassembled glycocalyx (LNPG) will restore normal venular permeability in a lipopolysaccharide (LPS)-induced sepsis model of mice.To test this hypothesis, we designed a unique perfusion microchamber in which permeability of isolated venules could be assessed by measuring the concentration of Evans blue dye (EBD) in microliter-samples of extravascular solution (ES).Histamine-induced time- and dose-dependent increases in EBD in the ES could be measured, confirming the sensitivity of the microchamber system. Notably, the histamine-induced increase in permeability was significantly attenuated by histamine receptor (H1) antagonist, triprolidine hydrochloride. Subsequently, mice were treated with LPS, or LPS + LNPG. Compared to control mice, venules from LPS-treated mice showed a significant increased permeability, which was significantly reduced by LNPG administration. Moreover, in the presence of wall shear stress, intraluminal administration of LNPG significantly reduced the permeability in isolated venules from LPS-treated mice. We have found no sex differences.Our newly developed microchamber system allows us to quantitatively measure the permeability of isolated mesenteric venules. LPS-induced sepsis increases permeability of venules that is attenuated by in vivo LNPG administration, which is also reestablished endothelial responses to shear stress. Thus, LNPG presents a promising therapeutic potential for restoring EG function and thereby mitigating vasogenic edema due to increased permeability in sepsis. Funding 2043457 /api/funding/2043457 false true Funding 2043458 /api/funding/2043458 false true true INTERNATIONAL 2024 true true true false true false PAY 2026-05-24 false 1 0 1 0 1 1 1 1 1 1 1 0 0 0 1 0 1 0 1 0 0 2 2 Folyóiratcikk Journal Article 24 1 Könyvrészlet Chapter in Book 25 0 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2025 0 1 0 1 0 Q1 false false false false 2024-07-21T06:24:17.910+0000 HUN-REN-SE Cerebrovaszkuláris és Neurokognitív ... (SE / AOK / I / KKI); Morfológiai és Fiziológiai Tanszék (SE / ETK2007); Sport- és Egészségtudományi Intézet (MTSE) 1 26 3 5 0 true 10025544 Language 10002 /api/language/10002 Angol English true 2 true PersonAuthorship 118038307 /api/authorship/118038307 1 0.2 true false false Ishiko Shinya true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 118038308 /api/authorship/118038308 2 0.2 false false false Author 10002221 /api/author/10002221 Koller Ákos true 10002221 true Koller Akos true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 118038309 /api/authorship/118038309 3 0.2 false false false Deng Wenseng true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 118038310 /api/authorship/118038310 4 0.2 false false false Huang An true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 118038311 /api/authorship/118038311 5 0.2 false true true Sun Dong true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PublicationIdentifier 26722855 /api/publicationidentifier/26722855 PlainSource 6 /api/publicationsource/6 PublicationSourceType 10001 /api/publicationsourcetype/10001 true true true DOI DOI https://doi.org/@@@ true true 6 true PAY IDENTICAL 10.1152/ajpheart.00138.2024 https://doi.org/10.1152/ajpheart.00138.2024 false true PublicationIdentifier 26989793 /api/publicationidentifier/26989793 PlainSource 1 /api/publicationsource/1 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true IDENTICAL 001293690000002 https://www.webofscience.com/wos/woscc/full-record/001293690000002 true true PublicationIdentifier 26908245 /api/publicationidentifier/26908245 PlainSource 3 /api/publicationsource/3 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true Scopus Scopus http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@ true true 3 true IDENTICAL 85199814077 http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85199814077 true true PublicationIdentifier 26722854 /api/publicationidentifier/26722854 PlainSource 17 /api/publicationsource/17 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true PubMed PubMed http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=@@@&dopt=Abstract true true 17 true IDENTICAL 38874615 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=38874615&dopt=Abstract false true Keyword 7880 /api/keyword/7880 true 7880 true Keyword 14223 /api/keyword/14223 true 14223 true Keyword 1017334 /api/keyword/1017334 true 1017334 true Keyword 1085814 /api/keyword/1085814 true 1085814 true Keyword 1534238 /api/keyword/1534238 true true SjrRating 11446455 /api/sjrrating/11446455 65 0.24 journal RatingType 10002 /api/ratingtype/10002 sjr true true ClassificationExternal 2705 /api/classificationexternal/2705 true 2705 true Q1 DIRECT true true /api/publication/35069431 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" >Ishiko Shinya </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10002221"><a href="/gui2/?type=authors&mode=browse&sel=10002221" target="_blank">Koller Akos (<span class="authorship-author-name">Koller Ákos</span> <span class="authorAux-mtmt"> Szív és vérkeringés</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Egészségtudományi Kar">ETK2007</span>/Morfológiai és Fiziológiai Tanszék; <span title="Magyar Testnevelési és Sporttudományi Egyetem">MTSE</span>/Sport- és Egészségtudományi Intézet; <span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/<span title="Transzlációs Medicina Intézet">TMI</span>/HUN-REN-SE Cerebrovaszkuláris és Neurokognitív Betegségek Kutatócsoport</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Deng Wenseng </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Huang An </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Sun Dong ✉ </span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;35069431" target="_blank">Liposomal nanocarriers of preassembled glycocalyx restore normal venular permeability and shear stress sensitivity in sepsis: assessed quantitatively with a novel microchamber system</a></div> <div> <span class="journal-title">AMERICAN JOURNAL OF PHYSIOLOGY: HEART AND CIRCULATORY PHYSIOLOGY</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/0363-6135">0363-6135</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1522-1539">1522-1539</a>)</span>: <span class="journal-volume">327</span> <span class="journal-issue">2</span> <span class="page"> pp H390-H398 </span> <span class="year">(2024)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_PAY" title=" Fizetős "> <a style="color:blue" title="10.1152/ajpheart.00138.2024" target="_blank" href="https://doi.org/10.1152/ajpheart.00138.2024"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="001293690000002" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/001293690000002"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="85199814077" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85199814077"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="38874615" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=38874615&dopt=Abstract"> PubMed </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Folyóirat szakterülete: Scopus - Cardiology and Cardiovascular Medicine&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Physiology (medical)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Physiology&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 1</span> | Független: 0 | Függő: 1 | Nem jelölt: 0 | WoS jelölt: 1 | Scopus jelölt:&nbsp;1 | WoS/Scopus jelölt:&nbsp;1 | DOI jelölt:&nbsp;1 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;35069431&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 2 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 35069431</span> | <span class="status-data status-VALIDATED"> Egyeztetett </span> Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">PubMed XML</span> </div> <div class="funder"> (Post-Covid 2021–34.) Támogató: MTA, TKP2021-EGA-37(TKP2021-EGA-37) </div> <div class="lastModified">Utolsó módosítás: 2025.02.12. 15:07 Bara Eszter (admin) </div> <div class="lockedBy">Központi kezelésű 2025.06.05. 01:43 Lévayné Deseő Katalin (MTMT Központi admin) </div> </div></div>