https://m2.mtmt.hu/ eng 2026-03-06 00:06 JournalArticle 35052153 VALIDATED true 0 false 2026-01-01T21:26:26.865+0000 2025-09-17T15:13:11.628+0000 2024-06-17T12:45:11.541+0000 true 10069606 Pintér Ibolya Katalin /api/admin/10069606 Admin true 2025-09-17T15:13:06.133+0000 2025-09-17T15:13:06.133+0000 2025-09-17T15:13:11.906+0000 true 10026589 Tichy Rita /api/admin/10026589 Admin true 10026589 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Veszelyi, Krisztina Subcellular Localization of Thioredoxin/Thioredoxin Reductase System—A Missing Link in Endoplasmic Reticulum Redox Balance true true /api/journal/10003252 REVIEWED true false 10003252 false 10003252 true 1661-6596 1422-0067 Journal FOREIGN 25 12 6647 6647 20 2024 The lumen of the endoplasmic reticulum (ER) is usually considered an oxidative environment; however, oxidized thiol-disulfides and reduced pyridine nucleotides occur there parallelly, indicating that the ER lumen lacks components which connect the two systems. Here, we investigated the luminal presence of the thioredoxin (Trx)/thioredoxin reductase (TrxR) proteins, capable of linking the protein thiol and pyridine nucleotide pools in different compartments. It was shown that specific activity of TrxR in the ER is undetectable, whereas higher activities were measured in the cytoplasm and mitochondria. None of the Trx/TrxR isoforms were expressed in the ER by Western blot analysis. Co-localization studies of various isoforms of Trx and TrxR with ER marker Grp94 by immunofluorescent analysis further confirmed their absence from the lumen. The probability of luminal localization of each isoform was also predicted to be very low by several in silico analysis tools. ER-targeted transient transfection of HeLa cells with Trx1 and TrxR1 significantly decreased cell viability and induced apoptotic cell death. In conclusion, the absence of this electron transfer chain may explain the uncoupling of the redox systems in the ER lumen, allowing parallel presence of a reduced pyridine nucleotide and a probably oxidized protein pool necessary for cellular viability. Funding 2041338 /api/funding/2041338 false true true true 2024 true true true false true false GOLD 2026-01-01 true https://doi.org/10.3390/ijms25126647 1 0 1 1 0 1 1 1 0 1 1 1 1 1 0 0 1 1 1 1 0 11 11 Folyóiratcikk Journal Article 24 1 Könyvrészlet Chapter in Book 25 0 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2025 0 1 1 1 1 D1 false false false false 2024-06-19T09:27:34.979+0000 Belgyógyászati és Hematológiai Klinika (SE / AOK / K); Molekuláris Biológiai Tanszék (SE / AOK / I / BMBI); Transzlációs Medicina Intézet (SE / AOK / I) 6 18 3 6 0 true Language 10002 /api/language/10002 Angol English true 2 true PersonAuthorship 117941371 /api/authorship/117941371 1 0.167 true false Author 10074867 /api/author/10074867 Veszelyi Krisztina Nóra true true Veszelyi Krisztina true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 117941372 /api/authorship/117941372 2 0.167 false false Author 10023838 /api/author/10023838 Czegle Ibolya true 10023838 true Czegle Ibolya true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 117941373 /api/authorship/117941373 3 0.167 false false Author 10061708 /api/author/10061708 Varga Viola true 10061708 true Varga Viola true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 117941374 /api/authorship/117941374 4 0.167 false false Author 10054897 /api/author/10054897 Németh Csilla Emese true 10054897 true Németh Csilla E. true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 117941375 /api/authorship/117941375 5 0.167 false false Author 10068063 /api/author/10068063 Besztercei Balázs true true Besztercei Balázs true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 117941376 /api/authorship/117941376 6 0.167 false true true Author 10015704 /api/author/10015704 Margittai Éva true 10015704 true Margittai Éva true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PublicationIdentifier 26680513 /api/publicationidentifier/26680513 PlainSource 6 /api/publicationsource/6 PublicationSourceType 10001 /api/publicationsourcetype/10001 true true true DOI DOI https://doi.org/@@@ true true 6 true GOLD true IDENTICAL 10.3390/ijms25126647 https://doi.org/10.3390/ijms25126647 false true PublicationIdentifier 26763282 /api/publicationidentifier/26763282 PlainSource 1 /api/publicationsource/1 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true IDENTICAL 001255985900001 https://www.webofscience.com/wos/woscc/full-record/001255985900001 true true PublicationIdentifier 26775237 /api/publicationidentifier/26775237 PlainSource 3 /api/publicationsource/3 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true Scopus Scopus http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@ true true 3 true IDENTICAL 85197127393 http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85197127393 true true PublicationIdentifier 26763291 /api/publicationidentifier/26763291 PlainSource 17 /api/publicationsource/17 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true PubMed PubMed http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=@@@&dopt=Abstract true true 17 true IDENTICAL 38928353 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=38928353&dopt=Abstract true true Keyword 4978 /api/keyword/4978 true 4978 true Keyword 1501400 /api/keyword/1501400 true true Keyword 1521485 /api/keyword/1521485 true true Keyword 3482923 /api/keyword/3482923 true true SjrRating 11489344 /api/sjrrating/11489344 18 0.1 journal RatingType 10002 /api/ratingtype/10002 sjr true true ClassificationExternal 1605 /api/classificationexternal/1605 true 1605 true D1 DIRECT true true /api/publication/35052153 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10074867"><a href="/gui2/?type=authors&mode=browse&sel=10074867" target="_blank">Veszelyi Krisztina (<span class="authorship-author-name">Veszelyi Krisztina Nóra</span> <span class="authorAux-mtmt"> molekuláris biológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis University">SU</span>/<span title="Faculty of Medicine">FM</span>/<span title="Instituties">I</span>/Transzlációs Medicina Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10023838"><a href="/gui2/?type=authors&mode=browse&sel=10023838" target="_blank">Czegle Ibolya (<span class="authorship-author-name">Czegle Ibolya</span> <span class="authorAux-mtmt"> Onkológia, belgyógyászat</span>) </a> </span> <span class="author-affil"><span title="Semmelweis University">SU</span>/<span title="Faculty of Medicine">FM</span>/<span title="Clinical">C</span>/Belgyógyászati és Hematológiai Klinika</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10061708"><a href="/gui2/?type=authors&mode=browse&sel=10061708" target="_blank">Varga Viola (<span class="authorship-author-name">Varga Viola</span> <span class="authorAux-mtmt"> Patobiokémia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis University">SU</span>/<span title="Faculty of Medicine">FM</span>/<span title="Instituties">I</span>/Transzlációs Medicina Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10054897"><a href="/gui2/?type=authors&mode=browse&sel=10054897" target="_blank">Németh Csilla E. (<span class="authorship-author-name">Németh Csilla Emese</span> <span class="authorAux-mtmt"> Biokémia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis University">SU</span>/<span title="Faculty of Medicine">FM</span>/<span title="Instituties">I</span>/<span title="Biokémiai és Molekuláris Biológiai Intézet">BMBI</span>/Molekuláris Biológiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10068063"><a href="/gui2/?type=authors&mode=browse&sel=10068063" target="_blank">Besztercei Balázs (<span class="authorship-author-name">Besztercei Balázs</span> <span class="authorAux-mtmt"> Élettan</span>) </a> </span> <span class="author-affil"><span title="Semmelweis University">SU</span>/<span title="Faculty of Medicine">FM</span>/<span title="Instituties">I</span>/Transzlációs Medicina Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10015704"><a href="/gui2/?type=authors&mode=browse&sel=10015704" target="_blank">Margittai Éva ✉ (<span class="authorship-author-name">Margittai Éva</span> <span class="authorAux-mtmt"> Molekuláris Biológiai</span>) </a> </span> <span class="author-affil"><span title="Semmelweis University">SU</span>/<span title="Faculty of Medicine">FM</span>/<span title="Instituties">I</span>/Transzlációs Medicina Intézet</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;35052153" target="_blank">Subcellular Localization of Thioredoxin/Thioredoxin Reductase System—A Missing Link in Endoplasmic Reticulum Redox Balance</a></div> <div> <span class="journal-title">INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/1661-6596">1661-6596</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1422-0067">1422-0067</a>)</span>: <span class="journal-volume">25</span> <span class="journal-issue">12</span> <span class="page"> Paper 6647. 20 p. </span> <span class="year">(2024)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Language: English | </span> <span class="identifiers"> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="10.3390/ijms25126647" target="_blank" href="https://doi.org/10.3390/ijms25126647"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="001255985900001" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/001255985900001"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="85197127393" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85197127393"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="38928353" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=38928353&dopt=Abstract"> PubMed </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Journal subject: Scopus - Organic Chemistry&nbsp;&nbsp;&nbsp;Rank:&nbsp;D1</div> <div class="journal-subject">Journal subject: Scopus - Spectroscopy&nbsp;&nbsp;&nbsp;Rank:&nbsp;D1</div> <div class="journal-subject">Journal subject: Scopus - Computer Science Applications&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q1</div> <div class="journal-subject">Journal subject: Scopus - Inorganic Chemistry&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q1</div> <div class="journal-subject">Journal subject: Scopus - Medicine (miscellaneous)&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q1</div> <div class="journal-subject">Journal subject: Scopus - Physical and Theoretical Chemistry&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q1</div> <div class="journal-subject">Journal subject: Scopus - Catalysis&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q2</div> <div class="journal-subject">Journal subject: Scopus - Molecular Biology&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q2</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="" class="citingPub-count">Citing papers: 1</span> | Independent citation: 1 | Self citation: 0 | Unknown citation: 0 | Number of citations in WoS: 1 | WoS/Scopus assigned:&nbsp;1 | Number of citations with DOI:&nbsp;1 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;35052153&sort=publishedYear,desc&sort=title"> Number of cited publications: 11 </a> </div> <div class="mtid"><span class="long-pub-mtid">Publication: 35052153</span> | <span class="status-data status-VALIDATED"> Validated </span> Core Citing | <span class="type-subtype">Journal Article ( Article ) </span> | <span class="pub-category">Scientific</span> | <span class="publication-sourceOfData">RIS-Egyéb</span> </div> <div class="funder"> (FK124442) Funder: NRDIO </div> <div class="lastModified">Last Modified: 2025.09.17. 17:13 Rita Tichy (SE_KK_Admin5_TR, admin) </div> </div></div>