https://m2.mtmt.hu/ hun 2026-09-20 23:37 JournalArticle 34875002 ADMIN_APPROVED true Faculty of Information Technology and Bionics, Pázmány Péter Catholic University, Budapest, Hungary Institute of Materials and Environmental Chemistry; Biological Nanochemistry Research Group, HUN-REN Research Centre for Natural Sciences, Budapest, Hungary Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary ELKH-SE Translational Extracellular Vesicle Research Group, Budapest, Hungary HCEMM-SE Extracellular Vesicle Research Group, Budapest, Hungary Department of Internal Medicine and Oncology, Division of Oncology, Semmelweis University, Budapest, Hungary Export Date: 1 July 2024 Correspondence Address: Garay, T.; Faculty of Information Technology and Bionics, Hungary; email: garay.tamas@itk.ppke.hu 0 false 2026-05-29T09:15:09.066+0000 2026-05-28T08:41:54.529+0000 2024-05-24T11:43:26.333+0000 true 10062250 Molnár-Taga Márta /api/admin/10062250 Admin true 10062250 2025-06-02T13:09:53.987+0000 2025-06-02T13:09:53.987+0000 2024-06-28T21:04:37.019+0000 true 10003133 Boros Annamária /api/admin/10003133 Admin true 10003133 Admin 10067876 /api/admin/10067876 Ladányi Gusztáv true true Admin 10014568 /api/admin/10014568 Vándor Andrea true 10014568 true 2024-10-17T16:44:08.551+0000 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Németh, Afrodité Extracellular vesicles promote migration despite BRAF inhibitor treatment in malignant melanoma cells. true true /api/journal/10016623 REVIEWED true false 10016623 false 10016623 true 1478-811X 1478-811X Journal FOREIGN 22 1 282 282 14 2024 Extracellular vesicles (EVs) constitute a vital component of intercellular communication, exerting significant influence on metastasis formation and drug resistance mechanisms. Malignant melanoma (MM) is one of the deadliest forms of skin cancers, because of its high metastatic potential and often acquired resistance to oncotherapies. The prevalence of BRAF mutations in MM underscores the importance of BRAF-targeted therapies, such as vemurafenib and dabrafenib, alone or in combination with the MEK inhibitor, trametinib. This study aimed to elucidate the involvement of EVs in MM progression and ascertain whether EV-mediated metastasis promotion persists during single agent BRAF (vemurafenib, dabrafenib), or MEK (trametinib) and combined BRAF/MEK (dabrafenib/trametinib) inhibition.Using five pairs of syngeneic melanoma cell lines, we assessed the impact of EVs - isolated from their respective supernatants - on melanoma cell proliferation and migration. Cell viability and spheroid growth assays were employed to evaluate proliferation, while migration was analyzed through mean squared displacement (MSD) and total traveled distance (TTD) measurements derived from video microscopy and single-cell tracking.Our results indicate that while EV treatments had remarkable promoting effect on cell migration, they exerted only a modest effect on cell proliferation and spheroid growth. Notably, EVs demonstrated the ability to mitigate the inhibitory effects of BRAF inhibitors, albeit they were ineffective against a MEK inhibitor and the combination of BRAF/MEK inhibitors. In summary, our findings contribute to the understanding of the intricate role played by EVs in tumor progression, metastasis, and drug resistance in MM. Funding 2040496 /api/funding/2040496 false true true true 2024 true true true false true false GOLD 2026-05-29 true https://doi.org/10.1186/s12964-024-01660-4 2 0 2 1 1 2 2 2 1 2 2 1 1 1 1 0 2 1 2 1 0 5 5 Folyóiratcikk Journal Article 24 2 Könyvrészlet Chapter in Book 25 0 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2025 0 1 0 1 0 2026 0 1 1 1 1 D1 false 10030126 false false false 2024-05-23T11:43:33.480+0000 Anyag- és Környezetkémiai Intézet (HRN TTK); Belgyógyászati és Onkológiai Klinika (SE / AOK / K); Biológiai Nanokémiai Kutatócsoport (HRN TTK / AKI); ELKH-SE Transzlációs Extracelluláris Vezikula K... (SE / AOK / I / GSII); Genetikai, Sejt- és Immunbiológiai Intézet (SE / AOK / I); HCEMM-SE Extracelluláris Vezikula Kutatócsoport (SE / AOK / I / GSII); Információs Technológiai és Bionikai Kar (PPKE); Roska Tamás Műszaki és Természettudományi Dokto... 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0.1 journal RatingType 10002 /api/ratingtype/10002 sjr true true ClassificationExternal 1303 /api/classificationexternal/1303 true 1303 true D1 DIRECT true true /api/publication/34875002 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10080217"><a href="/gui2/?type=authors&mode=browse&sel=10080217" target="_blank">Németh Afrodité (<span class="authorship-author-name">Németh Afrodité</span> <span class="authorAux-mtmt"> biológia</span>) </a> </span> <span class="author-affil"><span title="Pázmány Péter Katolikus Egyetem">PPKE</span>/<span title="Információs Technológiai és Bionikai Kar">ITK</span>/Roska Tamás Műszaki és Természettudományi Doktori Iskola</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10084057"><a href="/gui2/?type=authors&mode=browse&sel=10084057" target="_blank">Bányai Gréta L (<span class="authorship-author-name">Bányai Gréta Lilla</span> <span class="authorAux-mtmt"> biológia</span>) </a> </span> <span class="author-affil"><span title="Pázmány Péter Katolikus Egyetem">PPKE</span>/<span title="Információs Technológiai és Bionikai Kar">ITK</span>/Roska Tamás Műszaki és Természettudományi Doktori Iskola</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Dobos Nikolett K </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10105759"><a href="/gui2/?type=authors&mode=browse&sel=10105759" target="_blank">Kós Tamás (<span class="authorship-author-name">Kós Tamás</span> <span class="authorAux-mtmt"> immunológia, onkológia</span>) </a> </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10056875"><a href="/gui2/?type=authors&mode=browse&sel=10056875" target="_blank">Gaál Anikó (<span class="authorship-author-name">Gaál Anikó</span> <span class="authorAux-mtmt"> analitikai kémia</span>) </a> </span> <span class="author-affil"><span title="HUN-REN Természettudományi Kutatóközpont">HRN TTK</span>/<span title="Anyag- és Környezetkémiai Intézet">AKI</span>/Biológiai Nanokémiai Kutatócsoport</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10014807"><a href="/gui2/?type=authors&mode=browse&sel=10014807" target="_blank">Varga Zoltán (<span class="authorship-author-name">Varga Zoltán</span> <span class="authorAux-mtmt"> biofizika, kolloidkémia</span>) </a> </span> <span class="author-affil"><span title="HUN-REN Természettudományi Kutatóközpont">HRN TTK</span>/<span title="Anyag- és Környezetkémiai Intézet">AKI</span>/Biológiai Nanokémiai Kutatócsoport; <span title="HUN-REN Természettudományi Kutatóközpont">HRN TTK</span>/Anyag- és Környezetkémiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10015596"><a href="/gui2/?type=authors&mode=browse&sel=10015596" target="_blank">Buzás Edit I (<span class="authorship-author-name">Buzás Edit Irén</span> <span class="authorAux-mtmt"> Immunbiológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Genetikai, Sejt- és Immunbiológiai Intézet; <span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/<span title="Genetikai, Sejt- és Immunbiológiai Intézet">GSII</span>/ELKH-SE Transzlációs Extracelluláris Vezikula Kutatócsoport; <span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/<span title="Genetikai, Sejt- és Immunbiológiai Intézet">GSII</span>/HCEMM-SE Extracelluláris Vezikula Kutatócsoport</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10071730"><a href="/gui2/?type=authors&mode=browse&sel=10071730" target="_blank">Khamari Delaram (<span class="authorship-author-name">Khamari Delaram</span> <span class="authorAux-mtmt"> Extracellular vesicles</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Genetikai, Sejt- és Immunbiológiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10011481"><a href="/gui2/?type=authors&mode=browse&sel=10011481" target="_blank">Dank Magdolna (<span class="authorship-author-name">Dank Magdolna</span> <span class="authorAux-mtmt"> onkológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Klinikum">K</span>/Belgyógyászati és Onkológiai Klinika</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10024031"><a href="/gui2/?type=authors&mode=browse&sel=10024031" target="_blank">Takács István (<span class="authorship-author-name">Takács István</span> <span class="authorAux-mtmt"> Endokrinológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Klinikum">K</span>/Belgyógyászati és Onkológiai Klinika</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10023803"><a href="/gui2/?type=authors&mode=browse&sel=10023803" target="_blank">Szász A Marcell (<span class="authorship-author-name">Szász Attila Marcell</span> <span class="authorAux-mtmt"> emlőpatologia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Klinikum">K</span>/Belgyógyászati és Onkológiai Klinika</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10030126"><a href="/gui2/?type=authors&mode=browse&sel=10030126" target="_blank">Garay Tamás ✉ (<span class="authorship-author-name">Garay Tamás</span> <span class="authorAux-mtmt"> sejtbiológia, tumorbiológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Klinikum">K</span>/Belgyógyászati és Onkológiai Klinika; <span title="Pázmány Péter Katolikus Egyetem">PPKE</span>/Információs Technológiai és Bionikai Kar</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;34875002" target="_blank">Extracellular vesicles promote migration despite BRAF inhibitor treatment in malignant melanoma cells.</a></div> <div> <span class="journal-title">CELL COMMUNICATION AND SIGNALING</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/1478-811X">1478-811X</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1478-811X">1478-811X</a>)</span>: <span class="journal-volume">22</span> <span class="journal-issue">1</span> <span class="page"> Paper 282. 14 p. </span> <span class="year">(2024)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="10.1186/s12964-024-01660-4" target="_blank" href="https://doi.org/10.1186/s12964-024-01660-4"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="001229358300001" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/001229358300001"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="85193959183" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85193959183"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="38778340" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=38778340&dopt=Abstract"> PubMed </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Folyóirat szakterülete: Scopus - Biochemistry&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Cell Biology&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Molecular Biology&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 2</span> | Független: 1 | Függő: 1 | Nem jelölt: 0 | WoS jelölt: 2 | Scopus jelölt:&nbsp;1 | WoS/Scopus jelölt:&nbsp;2 | DOI jelölt:&nbsp;2 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;34875002&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 5 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 34875002</span> | <span class="status-data status-ADMIN_APPROVED"> Admin láttamozott </span> Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">kézi felvitel</span> </div> <div class="funder"> Thematic Excellence Program(TKP2021-EGA-42) </div> <div class="lastModified">Utolsó módosítás: 2026.05.28. 10:41 Kós Tamás (immunológia, onkológia) </div> <div class="lockedBy">Központi kezelésű 2024.10.17. 18:44 Ladányi Gusztáv (MTMT API user, admin) Vándor Andrea (VA, admin) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Megjegyzés</u>: Faculty of Information Technology and Bionics, Pázmány Péter Catholic University, Budapest, Hungary Institute of Materials and Environmental Chemistry; Biological Nanochemistry Research Group, HUN-REN Research Centre for Natural Sciences, Budapest, Hungary Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary ...</pre> </div></div>