https://m2.mtmt.hu/ hun 2026-05-26 01:49 JournalArticle 34850199 VALIDATED true 0 false 2026-05-23T19:55:00.613+0000 2026-02-11T12:52:55.353+0000 2024-05-13T08:32:50.240+0000 true 10086190 Bara Eszter /api/admin/10086190 Admin Bara Eszter (admin) true 2026-03-18T13:46:52.512+0000 2025-02-12T12:43:48.075+0000 true 10086190 Bara Eszter /api/admin/10086190 Admin Bara Eszter (admin) true Admin 10063514 /api/admin/10063514 Mikola Katalin (MTMT 3, admin) Mikola Katalin true 10063514 true 2026-01-21T13:12:53.998+0000 true false true 24 24 /api/publicationtype/24 PublicationType Folyóiratcikk 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType Folyóiratcikk 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType Szakcikk (Folyóiratcikk) 101 true 10000059 true 1 /api/category/1 Category Tudományos true 1 György, Bernadett true true /api/journal/10005621 REVIEWED CURRENT ISSUES IN MOLECULAR BIOLOGY 1467-3037 1467-3045 true false 10005621 false 10005621 true 1467-3037 1467-3045 Journal FOREIGN 46 5 4337 4357 4337 21 2024 The technical difficulty of separating extracellular vesicles (EVs) from plasma proteins in human blood presents a significant hurdle in EV research, particularly during nano ultra-high-performance liquid chromatography–tandem mass spectrometric (UHPLC-MS/MS) analysis, where detecting “vesicular” proteins among abundant plasma proteins is challenging. Standardisation is a pressing issue in EV research, prompting collaborative global efforts to address it. While the MISEV guidelines offer valuable recommendations, unanswered questions remain, particularly regarding sample storage. We compared size exclusion chromatography (SEC) columns with pore sizes of 35 nm and 70 nm to identify fractions with minimal contaminating proteins and the highest concentration of small EVs (sEVs). Following column selection, we explored potential differences in the quality and quantity of sEVs isolated from platelet-free plasma (PFP) after long-term storage at −80 °C (>2.5 years) compared to freshly drawn blood. Our methodologically rigorous study indicates that prolonged storage, under correct storage and processing conditions, does not compromise sEV quality. Both columns effectively isolated vesicles, with the 70 nm column exhibiting a higher abundance of “vesicular” proteins. We propose a relatively rapid and moderately efficient protocol for obtaining a comparatively pure sEV fraction from plasma, facilitating sEV processing in clinical trials. Funding 2040953 /api/funding/2040953 NKFIH(NVKP_16-1-2016-0004) false true Funding 2040954 /api/funding/2040954 ((NKFIH) [VEKOP-2.3.2-16-2016-00002) Támogató: NKFIH false true Funding 2040955 /api/funding/2040955 (STIA-KFI-2020) false true Funding 2040956 /api/funding/2040956 (VEKOP-2.3.3-15-2017-00016) false true Funding 2040957 /api/funding/2040957 (TKP2021-EGA-23) Támogató: Innovációs és Technológiai Minisztérium false true Funding 2040958 /api/funding/2040958 (RRF-2.3.1-21-2022-00003) false true Funding 2040959 /api/funding/2040959 (2019-2.1.7-ERA-NET-2021-00015) false true Funding 2040960 /api/funding/2040960 TKP2021-EGA-37(TKP2021-EGA-37) false true Funding 2040961 /api/funding/2040961 (142192) Támogató: OTKA false true true 2024 true true true false true false GOLD 2026-05-23 true https://doi.org/10.3390/cimb46050264 13 0 13 12 2 13 13 11 11 12 12 11 11 12 1 0 13 12 13 12 0 14 14 Folyóiratcikk Journal Article 24 12 Könyvrészlet Chapter in Book 25 1 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2024 0 1 1 1 1 2025 0 8 7 8 7 2026 0 4 4 4 4 Q2 false false false false 2024-05-12T08:32:57.251+0000 Doktori Iskola (MTSE); Genetikai, Sejt- és Immunbiológiai Intézet (SE / AOK / I); Magyar Testnevelési és Sporttudományi Egyetem; Molekuláris Edzésélettani Kutató Központ (MTSE / SETI); Növényélettani és Molekuláris Növénybiológiai T... (ELTE / TTK / Bio_I); Szerves Kémiai Intézet (HRN TTK) 8 57 6 8 0 true 10063514 Language 10002 /api/language/10002 Angol Angol English true 2 true PersonAuthorship 116573342 /api/authorship/116573342 György, Bernadett [György, Bernadett (Sporttudomány), szerző] Magyar Testnevelési és Sporttudományi Egyetem; Doktori Iskola (MTSE) 1 0.125 true false Author 10069850 /api/author/10069850 György Bernadett (Sporttudomány) György Bernadett true true György Bernadett true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 116573343 /api/authorship/116573343 Pálóczi, Krisztina* [Pálóczi, Krisztina (immunológia, gene...), szerző] Genetikai, Sejt- és Immunbiológiai Intézet (SE / AOK / I) 2 0.125 true false Author 10024466 /api/author/10024466 Pálóczi Krisztina (immunológia, genetika) Pálóczi Krisztina true 10024466 true Pálóczi Krisztina true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 116573344 /api/authorship/116573344 Balbisi, Mirjam [Balbisi, Mirjam (Kémia), szerző] Szerves Kémiai Intézet (HRN TTK) 3 0.125 false false false Author 10081651 /api/author/10081651 Balbisi Mirjam (Kémia) Balbisi Mirjam true true Balbisi Mirjam true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 116573345 /api/authorship/116573345 Turiák, Lilla [Turiák, Lilla (tömegspektrometri...), szerző] Szerves Kémiai Intézet (HRN TTK) 4 0.125 false false Author 10024570 /api/author/10024570 Turiák Lilla (tömegspektrometria, proteomika) Turiák Lilla true 10024570 true Turiák Lilla true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 116573346 /api/authorship/116573346 Drahos, László [Drahos, László (proteomika, tömeg...), szerző] Szerves Kémiai Intézet (HRN TTK) 5 0.125 false false false Author 10002323 /api/author/10002323 Drahos László (proteomika, tömegspektrometria) Drahos László true 10002323 true Drahos László true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 116573347 /api/authorship/116573347 Visnovitz, Tamás [Visnovitz, Tamás (Biológia), szerző] Genetikai, Sejt- és Immunbiológiai Intézet (SE / AOK / I); Növényélettani és Molekuláris Növénybiológiai T... (ELTE / TTK / Bio_I) 6 0.125 false false Author 10026962 /api/author/10026962 Visnovitz Tamás (Biológia) Visnovitz Tamás true 10026962 true Visnovitz Tamás true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 116573348 /api/authorship/116573348 Koltai, Erika [Koltai, Erika (sporttudomány), szerző] Molekuláris Edzésélettani Kutató Központ (MTSE / SETI) 7 0.125 false false false Author 10022305 /api/author/10022305 Koltai Erika (sporttudomány) Koltai Erika true 10022305 true Koltai Erika true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 116573349 /api/authorship/116573349 Radák, Zsolt ✉ [Radák, Zsolt (Sporttudomány), szerző] Molekuláris Edzésélettani Kutató Központ (MTSE / SETI) 8 0.125 false true true Author 10003262 /api/author/10003262 Radák Zsolt (Sporttudomány) Radák Zsolt true 10003262 true Radák Zsolt true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PublicationIdentifier 26171261 /api/publicationidentifier/26171261 DOI: 10.3390/cimb46050264 PlainSource 6 /api/publicationsource/6 DOI PublicationSourceType 10001 /api/publicationsourcetype/10001 DOI true true true DOI DOI https://doi.org/@@@ true true 6 true GOLD true IDENTICAL 10.3390/cimb46050264 https://doi.org/10.3390/cimb46050264 false true PublicationIdentifier 26308050 /api/publicationidentifier/26308050 WoS: 001233865800001 PlainSource 1 /api/publicationsource/1 WoS PublicationSourceType 10003 /api/publicationsourcetype/10003 Indexelő adatbázis false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true IDENTICAL 001233865800001 https://www.webofscience.com/wos/woscc/full-record/001233865800001 false true PublicationIdentifier 26302894 /api/publicationidentifier/26302894 Scopus: 85194230151 PlainSource 3 /api/publicationsource/3 Scopus PublicationSourceType 10003 /api/publicationsourcetype/10003 Indexelő adatbázis false true true Scopus Scopus http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@ true true 3 true IDENTICAL 85194230151 http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85194230151 false true PublicationIdentifier 26253927 /api/publicationidentifier/26253927 PubMed: 38785532 PlainSource 17 /api/publicationsource/17 PubMed PublicationSourceType 10003 /api/publicationsourcetype/10003 Indexelő adatbázis false true true PubMed PubMed http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=@@@&dopt=Abstract true true 17 true IDENTICAL 38785532 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=38785532&dopt=Abstract true true PublicationIdentifier 26171262 /api/publicationidentifier/26171262 Egyéb URL: https://www.mdpi.com/1467-3045/46/5/264 PlainSource 40 /api/publicationsource/40 Egyéb URL PublicationSourceType 10006 /api/publicationsourcetype/10006 Link true true true Egyéb URL Other URL @@@ true true 40 true IDENTICAL https://www.mdpi.com/1467-3045/46/5/264 https://www.mdpi.com/1467-3045/46/5/264 false true SjrRating 11483382 /api/sjrrating/11483382 sjr:Q2 (2024) Scopus - Medicine (miscellaneous) CURRENT ISSUES IN MOLECULAR BIOLOGY 1467-3037 1467-3045 781 0.49 journal RatingType 10002 /api/ratingtype/10002 sjr sjr true true ClassificationExternal 2701 /api/classificationexternal/2701 Scopus - Medicine (miscellaneous) true 2701 true Q2 DIRECT true true Reference 52859920 /api/reference/52859920 1. 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(2024) CURRENT ISSUES IN MOLECULAR BIOLOGY 1467-3037 1467-3045 46 5 4337-4357<div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10069850"><a href="/gui2/?type=authors&mode=browse&sel=10069850" target="_blank">György Bernadett (<span class="authorship-author-name">György Bernadett</span> <span class="authorAux-mtmt"> Sporttudomány</span>) </a> </span> <span class="author-affil">Magyar Testnevelési és Sporttudományi Egyetem; <span title="Magyar Testnevelési és Sporttudományi Egyetem">MTSE</span>/Doktori Iskola</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10024466"><a href="/gui2/?type=authors&mode=browse&sel=10024466" target="_blank">Pálóczi Krisztina^* (<span class="authorship-author-name">Pálóczi Krisztina</span> <span class="authorAux-mtmt"> immunológia, genetika</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Genetikai, Sejt- és Immunbiológiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10081651"><a href="/gui2/?type=authors&mode=browse&sel=10081651" target="_blank">Balbisi Mirjam (<span class="authorship-author-name">Balbisi Mirjam</span> <span class="authorAux-mtmt"> Kémia</span>) </a> </span> <span class="author-affil"><span title="HUN-REN Természettudományi Kutatóközpont">HRN TTK</span>/Szerves Kémiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10024570"><a href="/gui2/?type=authors&mode=browse&sel=10024570" target="_blank">Turiák Lilla (<span class="authorship-author-name">Turiák Lilla</span> <span class="authorAux-mtmt"> tömegspektrometria, proteomika</span>) </a> </span> <span class="author-affil"><span title="HUN-REN Természettudományi Kutatóközpont">HRN TTK</span>/Szerves Kémiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10002323"><a href="/gui2/?type=authors&mode=browse&sel=10002323" target="_blank">Drahos László (<span class="authorship-author-name">Drahos László</span> <span class="authorAux-mtmt"> proteomika, tömegspektrometria</span>) </a> </span> <span class="author-affil"><span title="HUN-REN Természettudományi Kutatóközpont">HRN TTK</span>/Szerves Kémiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10026962"><a href="/gui2/?type=authors&mode=browse&sel=10026962" target="_blank">Visnovitz Tamás (<span class="authorship-author-name">Visnovitz Tamás</span> <span class="authorAux-mtmt"> Biológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Genetikai, Sejt- és Immunbiológiai Intézet; <span title="Eötvös Loránd Tudományegyetem">ELTE</span>/<span title="Természettudományi Kar">TTK</span>/<span title="Biológiai Intézet">Bio_I</span>/Növényélettani és Molekuláris Növénybiológiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10022305"><a href="/gui2/?type=authors&mode=browse&sel=10022305" target="_blank">Koltai Erika (<span class="authorship-author-name">Koltai Erika</span> <span class="authorAux-mtmt"> sporttudomány</span>) </a> </span> <span class="author-affil"><span title="Magyar Testnevelési és Sporttudományi Egyetem">MTSE</span>/<span title="Sport- és Egészségtudományi Intézet">SETI</span>/Molekuláris Edzésélettani Kutató Központ</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10003262"><a href="/gui2/?type=authors&mode=browse&sel=10003262" target="_blank">Radák Zsolt ✉ (<span class="authorship-author-name">Radák Zsolt</span> <span class="authorAux-mtmt"> Sporttudomány</span>) </a> </span> <span class="author-affil"><span title="Magyar Testnevelési és Sporttudományi Egyetem">MTSE</span>/<span title="Sport- és Egészségtudományi Intézet">SETI</span>/Molekuláris Edzésélettani Kutató Központ</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;34850199" target="_blank">Effect of the 35 nm and 70 nm Size Exclusion Chromatography (SEC) Column and Plasma Storage Time on Separated Extracellular Vesicles</a></div> <div> <span class="journal-title">CURRENT ISSUES IN MOLECULAR BIOLOGY</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/1467-3037">1467-3037</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1467-3045">1467-3045</a>)</span>: <span class="journal-volume">46</span> <span class="journal-issue">5</span> <span class="page"> pp 4337-4357 </span> <span class="year">(2024)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="10.3390/cimb46050264" target="_blank" href="https://doi.org/10.3390/cimb46050264"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="001233865800001" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/001233865800001"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="85194230151" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85194230151"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="38785532" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=38785532&dopt=Abstract"> PubMed </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="https://www.mdpi.com/1467-3045/46/5/264" target="_blank" href="https://www.mdpi.com/1467-3045/46/5/264"> Egyéb URL </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Folyóirat szakterülete: Scopus - Medicine (miscellaneous)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q2</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Microbiology (medical)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q2</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Microbiology&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q2</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Molecular Biology&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q3</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 13</span> | Független: 12 | Függő: 1 | Nem jelölt: 0 | WoS jelölt: 11 | Scopus jelölt:&nbsp;11 | WoS/Scopus jelölt:&nbsp;12 | DOI jelölt:&nbsp;13 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;34850199&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 14 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 34850199</span> | <span class="status-data status-VALIDATED"> Egyeztetett </span> Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">kézi felvitel</span> </div> <div class="funder"> (NVKP_16-1-2016-0004) Támogató: NKFIH, (VEKOP-2.3.2-16-2016-00002) Támogató: NKFIH, (STIA-KFI-2020), (VEKOP-2.3.3.-15-2017-00016), (TKP2021-EGA-23) Támogató: Innovációs és Technológiai Minisztérium, (RRF-2.3.1-21-2022-00003), (2019-2.1.7-ERA-NET-2021-00015), TKP2021-EGA-37(TKP2021-EGA-37), (142192) Támogató: OTKA </div> <div class="lastModified">Utolsó módosítás: 2026.02.11. 13:52 Balázs András (MTMT Központ, admin) </div> <div class="lockedBy">Központi kezelésű 2026.01.21. 14:12 Mikola Katalin (MTMT 3, admin) </div> </div></div>