Certain genetic factors, including single-nucleotide polymorphisms (SNPs) in the SIRT1
gene, have been linked to medication-related osteonecrosis of the jaw (MRONJ). This
study examined four SNPs in the SIRT1 gene and implemented multivariate statistical
analysis to analyze genetic and clinical factors in MRONJ patients. Genomic DNA was
isolated from peripheral blood samples of 63 patients of European origin treated for
MRONJ, and four SNP genotypes in the gene encoding the SIRT-1 protein were determined
by Sanger sequencing. The allele frequencies measured in the MRONJ population were
compared with allele frequencies measured in the European population in the National
Center for Biotechnology Information Allele Frequency Aggregator (NCBI ALFA) database.
Genetic and clinical factors were examined with multivariate statistical analysis.
A C:A allele distribution ratio of 77.8:22.2 was measured in the rs932658 SNP. In
the ALFA project, a C:A allele distribution ratio of 59.9:40.1 was detected in the
European population, which was found to be a significant difference (p = 4.5 × 10−5).
Multivariate statistical analysis revealed a positive correlation (0.275) between
the genotype of SNP rs932658 and the number of stages improved during appropriate
MRONJ therapy. It is concluded that allele A in SNP rs932658 in the SIRT1 gene acts
as a protective factor in MRONJ.
