(TKP2021-EGA-23) Támogató: Innovációs és Technológiai Minisztérium
(STIAKFI007)
(Higher Education Institutional Excellence Program of the Ministry for Innovation
and Technology in Hungary, within the framework of the Molecular Biology thematic
program of the Semmelweis University)
Szakterületek:
Tudomány
In this present study, the material science background of crosslinked gelatin (GEL)
was investigated. The aim was to assess the optimal reaction parameters for the production
of a water-insoluble crosslinked gelatin matrix suitable for heat sterilization. Matrices
were subjected to enzymatic degradation assessments, and their ability to withstand
heat sterilization was evaluated. The impact of different crosslinkers on matrix properties
was analyzed. It was found that matrices crosslinked with butanediol diglycidyl ether
(BDDE) and poly(ethylene glycol) diglycidyl ether (PEGDE) were resistant to enzymatic
degradation and heat sterilization. Additionally, at 1 v/v % crosslinker concentration,
the crosslinked weight was lower than the starting weight, suggesting simultaneous
degradation and crosslinking. The crosslinked weight and swelling ratio were optimal
in the case of the matrices that were crosslinked with 3% and 5% v/v BDDE and PEGDE.
FTIR analysis confirmed crosslinking, and the reduction of free primary amino groups
indicated effective crosslinking even at a 1% v/v crosslinker concentration. Moreover,
stress–strain and compression characteristics of the 5% v/v BDDE crosslinked matrix
were comparable to native gelatin. Based on material science measurements, the crosslinked
matrices may be promising candidates for scaffold development, including properties
such as resistance to enzymatic degradation and heat sterilization.
