Hungarian National Research, Development and Innovation Office (NRDI Fund)(133837)
(133837)
Hungarian Brain Research Program(NAP2022-I-1/2022)
(NAP2022-I-1/2022)
(2017-1.2.1-NKP-2017-00002.)
MILAB(RRF-2.3.1-21-2022-00004) Támogató: NKFIH
(ÚNKP-19-2-I-SE-36)
(ÚNKP-18-2-I-SE-20)
(ÚNKP-19-3-I-SE-9)
(ÚNKP-23-3-I-SE-48)
Recovery and Resilience Facility of the European Union within the framework of Program
Széchenyi ...(RRF-2.3.1-21-2022-00011)
National Laboratory of Translational Neuroscience (TINL)(RRF-2.3.1-21-2022-00011)
(RRF-2.3.1-21-2022-00011)
GABAergic inhibitory neurons fundamentally shape the activity and plasticity of cortical
circuits. A major subset of these neurons contains somatostatin (SOM); these cells
play crucial roles in neuroplasticity, learning, and memory in many brain areas including
the hippocampus, and are implicated in several neuropsychiatric diseases and neurodegenerative
disorders. Two main types of SOM-containing cells in area CA1 of the hippocampus are
oriens-lacunosum-moleculare (OLM) cells and hippocampo-septal (HS) cells. These cell
types show many similarities in their soma-dendritic architecture, but they have different
axonal targets, display different activity patterns in vivo, and are thought to have
distinct network functions. However, a complete understanding of the functional roles
of these interneurons requires a precise description of their intrinsic computational
properties and their synaptic interactions. In the current study we generated, analyzed,
and make available several key data sets that enable a quantitative comparison of
various anatomical and physiological properties of OLM and HS cells in mouse. The
data set includes detailed scanning electron microscopy (SEM)-based 3D reconstructions
of OLM and HS cells along with their excitatory and inhibitory synaptic inputs. Combining
this core data set with other anatomical data, patch-clamp electrophysiology, and
compartmental modeling, we examined the precise morphological structure, inputs, outputs,
and basic physiological properties of these cells. Our results highlight key differences
between OLM and HS cells, particularly regarding the density and distribution of their
synaptic inputs and mitochondria. For example, we estimated that an OLM cell receives
about 8,400, whereas an HS cell about 15,600 synaptic inputs, about 16% of which are
GABAergic. Our data and models provide insight into the possible basis of the different
functionality of OLM and HS cell types and supply essential information for more detailed
functional models of these neurons and the hippocampal network.
