https://m2.mtmt.hu/ hun 2026-05-01 23:48 PublicationOther 34747676 ADMIN_APPROVED true 0 false 2026-04-27T06:54:29.064+0000 2024-06-20T07:50:47.233+0000 2024-03-20T12:33:06.655+0000 true 10069606 Pintér Ibolya Katalin /api/admin/10069606 Admin true 2024-03-20T12:33:17.701+0000 2024-03-20T12:33:17.701+0000 2024-06-20T07:50:47.241+0000 true 10015544 Csajbók Edit /api/admin/10015544 Admin true 10015544 true true 29 29 /api/publicationtype/29 PublicationType 15 true 29 PublicationOther true 10000261 Not classified true 29 29 /api/publicationtype/29 PublicationType 15 true 29 PublicationOther /api/subtype/10000261 Nem besorolt SubType 1585 true 10000261 true 1 /api/category/1 Category true 1 Babay, Imre SYSTEMIC INFLAMMATION INDUCED BY ANGIOTENSIN II INCREASES C3a-MEDIATED VASOCONSTRICTION IN MICE 189 189 189 1 2023 Introduc�on: Cardiovascular diseases are known to be associated with ac�va�on of the complement system, however, the precise rela�onship between the two processes is partly unclear. The complement component C3a receptor (C3aR) has been iden�fied in various cell types within the mouse arterial wall and the vasoconstrictor effect of C3a may be mediated by resident macrophages in the adven��a via thromboxane A2 (TXA2) release, as found by our workgroup in previous research. Aims: The primary objec�ve of this study was to determine the effects of chronic angiotensin II (Ang II) administra�on on C3a-induced vasoconstric�on in mice, along with demonstra�ng the presence of macrophages in the adven��a using alterna�ve methods. Methods: Changes in the isometric tension of the vascular segments were measured using myography a�er isola�ng thoracic and abdominal aor�c segments of adult male C57BL/6 mice. The mice were infused with either Ang II (520 ng/kg/min) or saline for 14 days via microosmo�c pumps implanted in the subscapular region. qPCR assays were performed to measure the expression level of C3aR and the mouse macrophage marker F4/80. The expression of other genes involved in the signaling pathway, such as Cyclooxygenase 1 and 2 (Cox1 and Cox2), thromboxane A synthase 1 (Tbxas1), and thromboxane A2 receptor (Tbxa2r), were inves�gated. The localiza�on of C3AR1 was confirmed using immunohistochemistry. The mechanism of the contrac�on was examined using the cyclooxygenase (COX) inhibitor indomethacin and SQ29548, a thromboxane prostanoid (TP) receptor antagonist. Results: An increased response to C3a (63–77) was observed in the vascular segments due to the increased expression of C3aR caused by Ang II treatment compared to controls that received saline infusion. The qPCR results revealed an increase in the expression of F4/80 and C3aR in the aortas, par�cularly in the adven��a, due to Ang II infusion. A�er removing the endothelium, the vasoconstric�on increased, while the vessels became unresponsive to C3a a�er the administra�on of COX and TP receptor inhibitors and the removal of the adven��a. Conclusion: It can be concluded from our results that chronic Ang II administra�on can lead to an enhanced vasoconstrictor response to C3a and an increased expression of C3aR in the aor�c wall, par�cularly in the adven��a where macrophages accumulate. Our findings propose that the vasoconstric�on induced by C3a in the mouse aorta is facilitated by macrophages that have gathered in the adven��a. During the development of various cardiovascular diseases, the effects of anaphylatoxins could be amplified and may contribute to the progression of the diseases Funding 2038321 /api/funding/2038321 false true Funding 2038322 /api/funding/2038322 false true Funding 2038323 /api/funding/2038323 false true Funding 2038324 /api/funding/2038324 false true Funding 2038325 /api/funding/2038325 false true Funding 2038326 /api/funding/2038326 false true true true 2024 true false false true false NONE 2026-04-27 false 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 false 10009921 false false false 2024-06-18T11:33:17.376+0000 ELKH-SE Cerebrovaszkuláris és Neurokognitív Bet... (SE / AOK / I / TMI); Transzlációs Medicina Intézet (SE / AOK / I) 6 29 2 6 0 false Language 10002 /api/language/10002 Angol English true 2 true PersonAuthorship 115861652 /api/authorship/115861652 1 0.16666667 true false Author 10080442 /api/author/10080442 Babay Imre true true Babay Imre true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 115861653 /api/authorship/115861653 2 0.16666667 false false Author 10069772 /api/author/10069772 Kerkovits Nóra Melinda true true Melinda Kerkovits Nóra true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 115861654 /api/authorship/115861654 3 0.16666667 false false Author 10086970 /api/author/10086970 Tóth Csillag Virág true true Virág Tóth Csillag true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 115861655 /api/authorship/115861655 4 0.16666667 false false Author 10027489 /api/author/10027489 Szénási Gábor true 10027489 true Szénási Gábor true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 115861656 /api/authorship/115861656 5 0.16666667 false false Author 10020915 /api/author/10020915 Kosztelnik Mónika true 10020915 true Kosztelnik Mónika true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 115861657 /api/authorship/115861657 6 0.16666667 false true Author 10009921 /api/author/10009921 Benyó Zoltán true 10009921 true Benyó Zoltán true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true /api/publication/34747676 <div class="PublicationOther Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10080442"><a href="/gui2/?type=authors&mode=browse&sel=10080442" target="_blank">Babay Imre (<span class="authorship-author-name">Babay Imre</span> <span class="authorAux-mtmt"> élettan, kórélettan</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Transzlációs Medicina Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10069772"><a href="/gui2/?type=authors&mode=browse&sel=10069772" target="_blank">Melinda Kerkovits Nóra (<span class="authorship-author-name">Kerkovits Nóra Melinda</span> <span class="authorAux-mtmt"> Élettan</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Transzlációs Medicina Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10086970"><a href="/gui2/?type=authors&mode=browse&sel=10086970" target="_blank">Virág Tóth Csillag (<span class="authorship-author-name">Tóth Csillag Virág</span> <span class="authorAux-mtmt"> élettan, kórélettan</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Transzlációs Medicina Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10027489"><a href="/gui2/?type=authors&mode=browse&sel=10027489" target="_blank">Szénási Gábor (<span class="authorship-author-name">Szénási Gábor</span> <span class="authorAux-mtmt"> kórélettan</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Transzlációs Medicina Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10020915"><a href="/gui2/?type=authors&mode=browse&sel=10020915" target="_blank">Kosztelnik Mónika (<span class="authorship-author-name">Kosztelnik Mónika</span> <span class="authorAux-mtmt"> Genetika</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Transzlációs Medicina Intézet; <span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/<span title="Transzlációs Medicina Intézet">TMI</span>/ELKH-SE Cerebrovaszkuláris és Neurokognitív Betegségek Kutatócsoport</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10009921"><a href="/gui2/?type=authors&mode=browse&sel=10009921" target="_blank">Benyó Zoltán (<span class="authorship-author-name">Benyó Zoltán</span> <span class="authorAux-mtmt"> Élettan és kórélettan</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Transzlációs Medicina Intézet; <span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/<span title="Transzlációs Medicina Intézet">TMI</span>/ELKH-SE Cerebrovaszkuláris és Neurokognitív Betegségek Kutatócsoport</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;34747676" target="_blank">SYSTEMIC INFLAMMATION INDUCED BY ANGIOTENSIN II INCREASES C3a-MEDIATED VASOCONSTRICTION IN MICE</a></div> <div class="description"> </div> <div class="country"> Megjelenés: <span class="pageLength">1 p.</span> <span class="publishedYear">(2023)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol </span> <span class="identifiers"> </span> <div class="mtid"><span class="long-pub-mtid">Közlemény: 34747676</span> | <span class="status-data status-ADMIN_APPROVED"> Admin láttamozott </span> Forrás | <span class="type-subtype">Egyéb ( Nem besorolt ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">kézi felvitel</span> </div> <div class="funder"> (K-125174) Támogató: OTKA, (K-135683) Támogató: OTKA, (K-139230) Támogató: NKFIH, (2020-1.1.6-JÖVŐ-2021-00010), Az orvos-, egészségtudományi- és gyógyszerészképzés tudományos műhelyeinek fejlesztése(EFOP-3.6.3-VEKOP-16-2017-00009) Támogató: EFOP-VEKOP, (TKP2021-EGA-25) </div> <div class="lastModified">Utolsó módosítás: 2024.06.20. 09:50 Csajbók Edit (SE_KK_Admin5_CSE, admin) </div> </div></div>