(1/00007/2005) Támogató: Nemzeti Kutatási, Fejlesztési és Innovációs Hivatal
(01063) Támogató: MTA
Visualization of biomolecules in their native environment or imaging-aided understanding
of more complex biomolecular processes are one of the focus areas of chemical biology
research, which requires selective, often site-specific labeling of targets. This
challenging task is effectively addressed by bioorthogonal chemistry tools in combination
with advanced synthetic biology methods. Today, the smart combination of the elements
of the bioorthogonal toolbox allows selective installation of multiple markers to
selected targets, enabling multicolor or multimodal imaging of biomolecules. Furthermore,
recent developments in bioorthogonally applicable probe design that meet the growing
demands of superresolution microscopy enable more complex questions to be addressed.
These novel, advanced probes enable highly sensitive, low-background, single- or multiphoton
imaging of biological species and events in live organisms at resolutions comparable
to the size of the biomolecule of interest. Herein, the latest developments in bioorthogonal
fluorescent probe design and labeling schemes will be discussed in the context of
in cellulo/in vivo (multicolor and/or superresolved) imaging schemes. The second part
focuses on the importance of genetically engineered minimal bioorthogonal tags, with
a particular interest in site-specific protein tagging applications to answer biological
questions.