Extracellular vesicles (EVs), through their complex cargo, can reflect the state of
their cell of origin and change the functions and phenotypes of other cells. These
features indicate strong biomarker and therapeutic potential and have generated broad
interest, as evidenced by the steady year‐on‐year increase in the numbers of scientific
publications about EVs. Important advances have been made in EV metrology and in understanding
and applying EV biology. However, hurdles remain to realising the potential of EVs
in domains ranging from basic biology to clinical applications due to challenges in
EV nomenclature, separation from non‐vesicular extracellular particles, characterisation
and functional studies. To address the challenges and opportunities in this rapidly
evolving field, the International Society for Extracellular Vesicles (ISEV) updates
its ‘Minimal Information for Studies of Extracellular Vesicles’, which was first published
in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the
current document, MISEV2023, is to provide researchers with an updated snapshot of
available approaches and their advantages and limitations for production, separation
and characterisation of EVs from multiple sources, including cell culture, body fluids
and solid tissues. In addition to presenting the latest state of the art in basic
principles of EV research, this document also covers advanced techniques and approaches
that are currently expanding the boundaries of the field. MISEV2023 also includes
new sections on EV release and uptake and a brief discussion of in vivo approaches
to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers,
this document conveys the current state of EV research to facilitate robust scientific
discoveries and move the field forward even more rapidly.