https://m2.mtmt.hu/ hun 2026-09-21 08:46 JournalArticle 34523104 VALIDATED true Research Faculty of Agriculture, Hokkaido University, Sapporo, 060-8589, Japan Graduate School of Global Food Resources, Hokkaido University, Sapporo, 060-0809, Japan Department of Molecular Biology, Institute of Biochemistry and Molecular Biology, Semmelweis University, Budapest, 1094, Hungary CellFree Sciences Co., Ltd, Matsuyama, 790-8577, Japan Export Date: 01 February 2024; Cited By: 0; Correspondence Address: T.E. Takasuka; Research Faculty of Agriculture, Hokkaido University, Sapporo, 060-8589, Japan; email: takasuka@agr.hokudai.ac.jp 0 false 2026-08-12T07:20:22.432+0000 2024-02-14T12:42:22.483+0000 2024-01-22T12:46:46.842+0000 true 10080205 Szalóky-Siki Ágnes /api/admin/10080205 Admin true 2024-03-02T20:29:40.124+0000 2024-03-02T20:29:40.124+0000 2024-02-21T00:51:21.236+0000 true 521 Szuper Admin /api/admin/521 Admin true true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Banko, P. In vitro co-expression chromatin assembly and remodeling platform for plant histone variants true true /api/journal/10019966 REVIEWED true false 10019966 false 10019966 true 2045-2322 Journal FOREIGN 14 1 936 936 10 2024 Histone variants play a central role in shaping the chromatin landscape in plants, yet, how their distinct combinations affect nucleosome properties and dynamics is still largely elusive. To address this, we developed a novel chromatin assembly platform for Arabidopsis thaliana, using wheat germ cell-free protein expression. Four canonical histones and five reported histone variants were used to assemble twelve A. thaliana nucleosome combinations. Seven combinations were successfully reconstituted and confirmed by supercoiling and micrococcal nuclease (MNase) assays. The effect of the remodeling function of the CHR11-DDR4 complex on these seven combinations was evaluated based on the nucleosome repeat length and nucleosome spacing index obtained from the MNase ladders. Overall, the current study provides a novel method to elucidate the formation and function of a diverse range of nucleosomes in plants. © 2024, The Author(s). 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Molekuláris Biológiai Tanszék (SE / AOK / I / BMBI) 1 9 1 7 0 true Language 10002 /api/language/10002 Angol English true 2 true PersonAuthorship 114041493 /api/authorship/114041493 1 0.14285715 true false false Banko P. true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 114041494 /api/authorship/114041494 2 0.143 true false false Okimune K.-I. true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 114041495 /api/authorship/114041495 3 0.143 false false false Author 10049990 /api/author/10049990 Nagy Szilvia Krisztina true 10049990 true Nagy S.K. true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 114041496 /api/authorship/114041496 4 0.14285715 false false false Hamasaki A. true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 114041497 /api/authorship/114041497 5 0.14285715 false false false Morishita R. 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/api/classificationexternal/1000 true 1000 true Q1 DIRECT true true /api/publication/34523104 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" >Banko P. </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Okimune K.-I.<sup>*</sup> </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10049990"><a href="/gui2/?type=authors&mode=browse&sel=10049990" target="_blank">Nagy S.K. (<span class="authorship-author-name">Nagy Szilvia Krisztina</span> <span class="authorAux-mtmt"> Molekuláris biológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/<span title="Biokémiai és Molekuláris Biológiai Intézet">BMBI</span>/Molekuláris Biológiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Hamasaki A. </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Morishita R. </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Onouchi H. </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Takasuka T.E. ✉ </span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;34523104" target="_blank">In vitro co-expression chromatin assembly and remodeling platform for plant histone variants</a></div> <div> <span class="journal-title">SCIENTIFIC REPORTS</span> <span class="journal-issn">( <a target="_blank" href="https://portal.issn.org/resource/ISSN/2045-2322">2045-2322</a>)</span>: <span class="journal-volume">14</span> <span class="journal-issue">1</span> <span class="page"> Paper 936. 10 p. </span> <span class="year">(2024)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="10.1038/s41598-024-51460-6" target="_blank" href="https://doi.org/10.1038/s41598-024-51460-6"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="001139454600003" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/001139454600003"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="85181937806" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85181937806"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="38195981" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=38195981&dopt=Abstract"> PubMed </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Folyóirat szakterülete: Scopus - Multidisciplinary&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Szociológiai Tudományos Bizottság IXGJO SZTB [1901-]&nbsp;&nbsp;&nbsp;A nemzetközi</div> <div class="journal-subject">Regionális Tudományok Bizottsága IXGJO RTB [1901-]&nbsp;&nbsp;&nbsp;B nemzetközi</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 3</span> | Független: 3 | Függő: 0 | Nem jelölt: 0 | WoS jelölt: 2 | Scopus jelölt:&nbsp;2 | WoS/Scopus jelölt:&nbsp;3 | DOI jelölt:&nbsp;3 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;34523104&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 3 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 34523104</span> | <span class="status-data status-VALIDATED"> Egyeztetett </span> Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">Scopus</span> </div> <div class="funder"> (129083) Támogató: Nemzeti Kutatási, Fejlesztési és Innovációs Hivatal </div> <div class="lastModified">Utolsó módosítás: 2024.02.14. 13:42 Wolf György (MTMT Központi admin) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Megjegyzés</u>: Research Faculty of Agriculture, Hokkaido University, Sapporo, 060-8589, Japan Graduate School of Global Food Resources, Hokkaido University, Sapporo, 060-0809, Japan Department of Molecular Biology, Institute of Biochemistry and Molecular Biology, Semmelweis University, Budapest, 1094, Hungary CellFree Sciences Co., Ltd, M...</pre> </div></div>