Cerebral microvascular health is a key biomarker for the study of natural aging and
associated neurological diseases. Our aim is to quantify aging‐associated change of
microvasculature at diverse dimensions in mice brain. We used optical coherence tomography
(OCT) and two‐photon microscopy (TPM) to obtain nonaged and aged C57BL/6J mice cerebral
microvascular images in vivo. Our results indicated that artery & vein, arteriole
& venule, and capillary from nonaged and aged mice showed significant differences
in density, diameter, complexity, perimeter, and tortuosity. OCT angiography and TPM
provided the comprehensive quantification for arteriole and venule via compensating
the limitation of each modality alone. We further demonstrated that arteriole and
venule at specific dimensions exhibited negative correlations in most quantification
analyses between nonaged and aged mice, which indicated that TPM and OCT were able
to offer complementary vascular information to study the change of cerebral blood
vessels in aging.
