Radiolabeling of Platelets with 99mTc-HYNIC-Duramycin for In Vivo Imaging Studies

Merkel, K. [Merkel, Keresztély Géza (Farmakológia), author] Departmnet of Biophysics and Radiation Biology (SU / FM / I); Szöllősi, D. [Szöllősi, Dávid (biofizika), author] Departmnet of Biophysics and Radiation Biology (SU / FM / I); Horváth, I. [Horváth, Ildikó (Biofizika, Sugárb...), author] Departmnet of Biophysics and Radiation Biology (SU / FM / I); Jezsó, B. [Jezsó, Bálint (Sejtbiológia), author] BNK (IMEC); Institute of Materials and Environmental Chemistry; Baranyai, Z. [Baranyai, Zsolt (Sebészet), author] Sebészeti, Transzplantációs és Gasztroenterológ... (SU / FM / C); Szigeti, K. [Szigeti, Krisztián (Biofizika), author] Departmnet of Biophysics and Radiation Biology (SU / FM / I); ACF-SU In Vivo Imaging (SU / FM / I / DBRB); Varga, Z. ✉ [Varga, Zoltán (biofizika, kolloi...), author] Departmnet of Biophysics and Radiation Biology (SU / FM / I); BNK (IMEC); Institute of Materials and Environmental Chemistry; Hegedüs, I. [Hegedüs, Imre (műszaki kémia), author] Departmnet of Biophysics and Radiation Biology (SU / FM / I); ACF-SU In Vivo Imaging (SU / FM / I / DBRB); Padmanabhan, P.; Gulyás, B.; Bergmann, R. [Bergmann, Ralf Konrad (orvostudomány), author] Departmnet of Biophysics and Radiation Biology (SU / FM / I); Máthé, D. ✉ [Máthé, Domokos (Molekuláris képal...), author] Departmnet of Biophysics and Radiation Biology (SU / FM / I); ACF-SU In Vivo Imaging (SU / FM / I / DBRB)

English Article (Journal Article) Scientific
Published: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 1661-6596 1422-0067 24 (23) Paper: 17119 , 11 p. 2023
  • SJR Scopus - Inorganic Chemistry: D1
Identifiers
Fundings:
  • (ÚNKP-21-5)
  • Establishing the Hungarian Center of Excellence for Molecular Medicine in partnership with EMBL(739593) Funder: Horizon 2020
  • (TKP-Bioimaging-2020-4.1.1-TKP2020)
  • (2020.1.16-jövő-2021-00013)
  • (TKP2021-EGA-31)
  • HCEMM(2022-2.1.1-NL-2022-00005) Funder: NRDIO
  • HCEMM TKP(TKP-2021-EGA-05) Funder: NRDIO
Following the in vivo biodistribution of platelets can contribute to a better understanding of their physiological and pathological roles, and nuclear imaging methods, such as single photon emission tomography (SPECT), provide an excellent method for that. SPECT imaging needs stable labeling of the platelets with a radioisotope. In this study, we report a new method to label platelets with 99mTc, the most frequently used isotope for SPECT in clinical applications. The proposed radiolabeling procedure uses a membrane-binding peptide, duramycin. Our results show that duramycin does not cause significant platelet activation, and radiolabeling can be carried out with a procedure utilizing a simple labeling step followed by a size-exclusion chromatography-based purification step. The in vivo application of the radiolabeled human platelets in mice yielded quantitative biodistribution images of the spleen and liver and no accumulation in the lungs. The performed small-animal SPECT/CT in vivo imaging investigations revealed good in vivo stability of the labeling, which paves the way for further applications of 99mTc-labeled-Duramycin in platelet imaging.
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2025-04-10 19:22