Establishing the Hungarian Center of Excellence for Molecular Medicine in partnership
with EMBL(739593) Támogató: Horizon 2020
(TKP-Bioimaging-2020-4.1.1-TKP2020)
(2020.1.16-jövő-2021-00013)
(TKP2021-EGA-31)
HCEMM(2022-2.1.1-NL-2022-00005) Támogató: NKFIH
HCEMM TKP(TKP-2021-EGA-05) Támogató: NKFIH
Following the in vivo biodistribution of platelets can contribute to a better understanding
of their physiological and pathological roles, and nuclear imaging methods, such as
single photon emission tomography (SPECT), provide an excellent method for that. SPECT
imaging needs stable labeling of the platelets with a radioisotope. In this study,
we report a new method to label platelets with 99mTc, the most frequently used isotope
for SPECT in clinical applications. The proposed radiolabeling procedure uses a membrane-binding
peptide, duramycin. Our results show that duramycin does not cause significant platelet
activation, and radiolabeling can be carried out with a procedure utilizing a simple
labeling step followed by a size-exclusion chromatography-based purification step.
The in vivo application of the radiolabeled human platelets in mice yielded quantitative
biodistribution images of the spleen and liver and no accumulation in the lungs. The
performed small-animal SPECT/CT in vivo imaging investigations revealed good in vivo
stability of the labeling, which paves the way for further applications of 99mTc-labeled-Duramycin
in platelet imaging.
