(TKP2021-EGA-23) Funder: Ministry for Innovation and Technology
(RRF-2.3.1-21-2022-0000)
(2019-2.1.7-ERA-NET-2021-00015)
Subjects:
Cell biology, Microbiology
In the past decade, extracellular vesicles (EVs) have attracted substantial interest
in biomedicine. With progress in the field, we have an increasing understanding of
cellular responses to EVs. In this Technical Report, we describe the direct nanoinjection
of EVs into the cytoplasm of single cells of different cell lines. By using robotic
fluidic force microscopy (robotic FluidFM), nanoinjection of GFP positive EVs and
EV‐like particles into single live HeLa, H9c2, MDA‐MB‐231 and LCLC‐103H cells proved
to be feasible. This injection platform offered the advantage of high cell selectivity
and efficiency. The nanoinjected EVs were initially localized in concentrated spot‐like
regions within the cytoplasm. Later, they were transported towards the periphery of
the cells. Based on our proof‐of‐principle data, robotic FluidFM is suitable for targeting
single living cells by EVs and may lead to information about intracellular EV cargo
delivery at a single‐cell level.
