https://m2.mtmt.hu/ hun 2026-09-20 20:31 JournalArticle 34266900 VALIDATED true 0 false 2026-09-07T12:19:07.891+0000 2026-07-09T07:43:55.645+0000 2023-11-07T12:59:19.663+0000 true 10080205 Szalóky-Siki Ágnes /api/admin/10080205 Admin true 2026-08-26T08:41:27.467+0000 2026-07-09T07:43:55.970+0000 true 10101397 Tokai Tamás /api/admin/10101397 Admin true Admin 10003134 /api/admin/10003134 Wolf György true 10003134 true Admin 10014568 /api/admin/10014568 Vándor Andrea true 10014568 true 2024-01-08T08:54:03.688+0000 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Kurilla, A. Studying the Association of TKS4 and CD2AP Scaffold Proteins and Their Implications in the Partial Epithelial–Mesenchymal Transition (EMT) Process true true /api/journal/10003252 REVIEWED true false 10003252 false 10003252 true 1661-6596 1422-0067 Journal FOREIGN 24 20 15136 15136 17 2023 Colon cancer is a leading cause of death worldwide. Identification of new molecular factors governing the invasiveness of colon cancer holds promise in developing screening and targeted therapeutic methods. The Tyrosine Kinase Substrate with four SH3 domains (TKS4) and the CD2-associated protein (CD2AP) have previously been linked to dynamic actin assembly related processes and cancer cell migration, although their co-instructive role during tumor formation remained unknown. Therefore, this study was designed to investigate the TKS4-CD2AP interaction and study the interdependent effect of TKS4/CD2AP on oncogenic events. We identified CD2AP as a novel TKS4 interacting partner via co-immunoprecipitation-mass spectrometry methods. The interaction was validated via Western blot (WB), immunocytochemistry (ICC) and proximity ligation assay (PLA). The binding motif of CD2AP was explored via peptide microarray. To uncover the possible cooperative effects of TKS4 and CD2AP in cell movement and in epithelial-mesenchymal transition (EMT), we performed gene silencing and overexpressing experiments. Our results showed that TKS4 and CD2AP form a scaffolding protein complex and that they can regulate migration and EMT-related pathways in HCT116 colon cancer cells. This is the first study demonstrating the TKS4-CD2AP protein–protein interaction in vitro, their co-localization in intact cells, and their potential interdependent effects on partial-EMT in colon cancer. Funding 2033046 /api/funding/2033046 false true Funding 2033047 /api/funding/2033047 false true Funding 2033048 /api/funding/2033048 false true Funding 2033348 /api/funding/2033348 false true Funding 2033349 /api/funding/2033349 false true Funding 2033350 /api/funding/2033350 false true Funding 2038688 /api/funding/2038688 false true true 2023 true true true false true false GOLD 2026-09-07 true https://doi.org/10.3390/ijms242015136 11 0 11 8 3 11 11 11 10 11 11 8 8 8 3 0 11 8 11 8 0 9 9 Folyóiratcikk Journal Article 24 11 Könyvrészlet Chapter in Book 25 0 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2024 0 5 3 5 3 2025 0 3 2 3 2 2026 0 3 3 3 3 D1 false 10000649,10076637,10000695 false false false 2023-11-06T13:02:58.448+0000 Biológia Doktori Iskola (ELTE / TTK); Enzimológiai Intézet (TTK); Molekuláris Biológiai Tanszék (SE / AOK / I / BMBI) 8 30 3 9 0 true 10014568 10003134 Language 10002 /api/language/10002 Angol English true 2 true PersonAuthorship 111343524 /api/authorship/111343524 1 0.111 true false true Author 10073233 /api/author/10073233 Kurilla Anita true true Kurilla A. true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 111343525 /api/authorship/111343525 2 0.111 false false false Author 10076637 /api/author/10076637 László Loretta true true László L. true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 111343526 /api/authorship/111343526 3 0.111 false false false Author 10076635 /api/author/10076635 Takács Tamás true true Takács T. true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 111343527 /api/authorship/111343527 4 0.111 false false false Author 10082437 /api/author/10082437 Tilajka Álmos true true Tilajka Á. true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 111343528 /api/authorship/111343528 5 0.111 false false false Lukács L. true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 111343529 /api/authorship/111343529 6 0.111 false false false Author 10027845 /api/author/10027845 Novák Julianna true 10027845 true Novák J. true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 111343530 /api/authorship/111343530 7 0.111 false false false Author 10058394 /api/author/10058394 Pancsa Rita true 10058394 true Pancsa R. true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 111343531 /api/authorship/111343531 8 0.111 false false false Author 10000649 /api/author/10000649 Buday László true 10000649 true Buday L. true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 111343532 /api/authorship/111343532 9 0.111 false true true Author 10000695 /api/author/10000695 Vas Virág true 10000695 true Vas V. true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PublicationIdentifier 24701001 /api/publicationidentifier/24701001 PlainSource 6 /api/publicationsource/6 PublicationSourceType 10001 /api/publicationsourcetype/10001 true true true DOI DOI https://doi.org/@@@ true true 6 true GOLD true IDENTICAL 10.3390/ijms242015136 https://doi.org/10.3390/ijms242015136 false true PublicationIdentifier 24891095 /api/publicationidentifier/24891095 PlainSource 1 /api/publicationsource/1 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true IDENTICAL 001095375700001 https://www.webofscience.com/wos/woscc/full-record/001095375700001 true true PublicationIdentifier 29842970 /api/publicationidentifier/29842970 SwordSource 36 /api/publicationsource/36 PublicationSourceType 10007 /api/publicationsourcetype/10007 true true true REAL REAL https://real.mtak.hu/@@@ true true 36 true GOLD true 224309 https://real.mtak.hu/224309 false true PublicationIdentifier 24701000 /api/publicationidentifier/24701000 PlainSource 3 /api/publicationsource/3 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true Scopus Scopus http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@ true true 3 true IDENTICAL 85175272888 http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85175272888 false true PublicationIdentifier 24701013 /api/publicationidentifier/24701013 PlainSource 17 /api/publicationsource/17 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true PubMed PubMed http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=@@@&dopt=Abstract true true 17 true IDENTICAL 37894817 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=37894817&dopt=Abstract false true SjrRating 11388415 /api/sjrrating/11388415 7 0.1 journal RatingType 10002 /api/ratingtype/10002 sjr true true ClassificationExternal 1604 /api/classificationexternal/1604 true 1604 true D1 DIRECT true true /api/publication/34266900 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10073233"><a href="/gui2/?type=authors&mode=browse&sel=10073233" target="_blank">Kurilla A. ✉ (<span class="authorship-author-name">Kurilla Anita</span> <span class="authorAux-mtmt"> Biológiai tudományok</span>) </a> </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10076637"><a href="/gui2/?type=authors&mode=browse&sel=10076637" target="_blank">László L. (<span class="authorship-author-name">László Loretta</span> <span class="authorAux-mtmt"> természettudomány</span>) </a> </span> <span class="author-affil"><span title="Eötvös Loránd Tudományegyetem">ELTE</span>/<span title="Természettudományi Kar">TTK</span>/Biológia Doktori Iskola</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10076635"><a href="/gui2/?type=authors&mode=browse&sel=10076635" target="_blank">Takács T. (<span class="authorship-author-name">Takács Tamás</span> <span class="authorAux-mtmt"> Természettudomány</span>) </a> </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10082437"><a href="/gui2/?type=authors&mode=browse&sel=10082437" target="_blank">Tilajka Á. (<span class="authorship-author-name">Tilajka Álmos</span> <span class="authorAux-mtmt"> Orvostudomány</span>) </a> </span> <span class="author-affil"><span title="Természettudományi Kutatóközpont">TTK</span>/Enzimológiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Lukács L. </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10027845"><a href="/gui2/?type=authors&mode=browse&sel=10027845" target="_blank">Novák J. (<span class="authorship-author-name">Novák Julianna</span> <span class="authorAux-mtmt"> Genetika, immunológia</span>) </a> </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10058394"><a href="/gui2/?type=authors&mode=browse&sel=10058394" target="_blank">Pancsa R. (<span class="authorship-author-name">Pancsa Rita</span> <span class="authorAux-mtmt"> Biológia</span>) </a> </span> <span class="author-affil"><span title="Természettudományi Kutatóközpont">TTK</span>/Enzimológiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10000649"><a href="/gui2/?type=authors&mode=browse&sel=10000649" target="_blank">Buday L. (<span class="authorship-author-name">Buday László</span> <span class="authorAux-mtmt"> Biokémia</span>) </a> </span> <span class="author-affil"><span title="Természettudományi Kutatóközpont">TTK</span>/Enzimológiai Intézet; <span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/<span title="Biokémiai és Molekuláris Biológiai Intézet">BMBI</span>/Molekuláris Biológiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10000695"><a href="/gui2/?type=authors&mode=browse&sel=10000695" target="_blank">Vas V. ✉ (<span class="authorship-author-name">Vas Virág</span> <span class="authorAux-mtmt"> Őssejt biológia</span>) </a> </span> <span class="author-affil"><span title="Természettudományi Kutatóközpont">TTK</span>/Enzimológiai Intézet</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;34266900" target="_blank">Studying the Association of TKS4 and CD2AP Scaffold Proteins and Their Implications in the Partial Epithelial–Mesenchymal Transition (EMT) Process</a></div> <div> <span class="journal-title">INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/1661-6596">1661-6596</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1422-0067">1422-0067</a>)</span>: <span class="journal-volume">24</span> <span class="journal-issue">20</span> <span class="page"> Paper 15136. 17 p. </span> <span class="year">(2023)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="10.3390/ijms242015136" target="_blank" href="https://doi.org/10.3390/ijms242015136"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="001095375700001" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/001095375700001"> WoS </a> </span> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:black" title="224309" target="_blank" href="https://real.mtak.hu/224309"> REAL </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="85175272888" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85175272888"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="37894817" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=37894817&dopt=Abstract"> PubMed </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Folyóirat szakterülete: Scopus - Inorganic Chemistry&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Organic Chemistry&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Spectroscopy&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Computer Science Applications&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Medicine (miscellaneous)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Physical and Theoretical Chemistry&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Catalysis&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q2</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Molecular Biology&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q2</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 11</span> | Független: 8 | Függő: 3 | Nem jelölt: 0 | WoS jelölt: 11 | Scopus jelölt:&nbsp;10 | WoS/Scopus jelölt:&nbsp;11 | DOI jelölt:&nbsp;11 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;34266900&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 9 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 34266900</span> | <span class="status-data status-VALIDATED"> Egyeztetett </span> Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">Scopus</span> </div> <div class="funder"> (2020–1.1.6-JÖVŐ-2021-00004), (HunProtEx 2018-1.2.1-NKP-2018–00005) Támogató: NKFIH, (FIEK-16-1-2016-0005), (FK142285), (BO/00174/22), National Laboratory of Translational Neuroscience (TINL)(RRF-2.3.1-21-2022-00011), Nemzeti Gyógyszerkutatási és Fejlesztési Laboratórium (PharmaLab)(RRF-2.3.1-21-2022-00015) Támogató: NKFIH </div> <div class="lastModified">Utolsó módosítás: 2026.07.09. 09:43 Tokai Tamás (Központi 2-3, admin) </div> <div class="lockedBy">Központi kezelésű 2024.01.08. 09:54 Wolf György (MTMT Központi admin) Vándor Andrea (VA, admin) </div> </div></div>