Predictive Potential of RNA Polymerase B (II) Subunit 1 (RPB1) Cytoplasmic Aggregation for Neoadjuvant Chemotherapy Failure

Nagy-Mikó, Bence [Nagy-Mikó, Bence (Molekuláris biológia), author] Department of Biochemistry and Molecular Biology (SZTE / TTIK / BI); Németh-Szatmári, Orsolya [Szatmári, Orsolya (Molekuláris biológia), author] Department of Biochemistry and Molecular Biology (SZTE / TTIK / BI); Faragó-Mészáros, Réka; Csókási, Aliz; Bognár, Bence [Bognár, Bence (info-bionika (mol...), author] Department of Biochemistry and Molecular Biology (SZTE / TTIK / BI); Ördög, Nóra [Ördög, Nóra (molekuláris biológia), author] Department of Pathology (SZTE / ASZMS); Borsos, Barbara N. [Borsos, Barbara Nikolett (molekuláris biológia), author] Department of Pathology (SZTE / ASZMS); Majoros, Hajnalka [Majoros, Hajnalka (molekuláris biológia), author] Department of Pathology (SZTE / ASZMS); Ujfaludi, Zsuzsanna [Újfaludi, Zsuzsanna (molekuláris bioló...), author] Department of Pathology (SZTE / ASZMS); Oláh-Németh, Orsolya [Oláh, Orsolya (Pathologia, Kísér...), author] Department of Pathology (SZTE / ASZMS); Nikolényi, Aliz [Nikolényi, Alíz (sugárterápia, kli...), author] Department of Oncotherapy (SZTE / ASZMS); Dobi, Ágnes [Dobi, Ágnes (sugárterápia, kli...), author] Department of Oncotherapy (SZTE / ASZMS); Kószó, Renáta [Kószó, Renáta Lilla (sugárterápia), author] Department of Oncotherapy (SZTE / ASZMS); Sántha, Dóra [Sántha, Dóra (klinikai onkológia), author] Department of Oncotherapy (SZTE / ASZMS); Lázár, György [Lázár, György ifj (Klinikai orvostud...), author] Department of Surgery (SZTE / ASZMS); Simonka, Zsolt [Simonka, Zsolt (sebészet), author] Department of Surgery (SZTE / ASZMS); Paszt, Attila [Paszt, Attila (sebészet), author] Department of Surgery (SZTE / ASZMS); Ormándi, Katalin; Pankotai, Tibor [Pankotai, Tibor (molekuláris patol...), author] Department of Pathology (SZTE / ASZMS); Boros, Imre M. [Boros, Imre Miklós (Molekuláris biológia), author] Department of Biochemistry and Molecular Biology (SZTE / TTIK / BI); Villányi, Zoltán ✉ [Villanyi, Zoltan (Biokémia es molek...), author] Department of Biochemistry and Molecular Biology (SZTE / TTIK / BI); Vörös, András ✉ [Vörös, András (patológia), author] Department of Pathology (SZTE / ASZMS)

English Article (Journal Article) Scientific
Published: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 1661-6596 1422-0067 24 (21) Paper: 15869 , 9 p. 2023
  • SJR Scopus - Inorganic Chemistry: D1
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We aimed to investigate the contribution of co-translational protein aggregation to the chemotherapy resistance of tumor cells. Increased co-translational protein aggregation reflects altered translation regulation that may have the potential to buffer transcription under genotoxic stress. As an indicator for such an event, we followed the cytoplasmic aggregation of RPB1, the aggregation-prone largest subunit of RNA polymerase II, in biopsy samples taken from patients with invasive carcinoma of no special type. RPB1 frequently aggregates co-translationally in the absence of proper HSP90 chaperone function or in ribosome mutant cells as revealed formerly in yeast. We found that cytoplasmic foci of RPB1 occur in larger sizes in tumors that showed no regression after therapy. Based on these results, we propose that monitoring the cytoplasmic aggregation of RPB1 may be suitable for determining—from biopsy samples taken before treatment—the effectiveness of neoadjuvant chemotherapy.
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2024-12-02 09:52