Biofilm forming bacteria and archaea in thermal karst springs of Gellért Hill discharge area (Hungary)

Borsodi, AK [Kériné Borsodi, Andrea (Mikrobiológia), author] Danube Research Institute (CER); Department of Microbiology (ELTE / ELU FoS / Bio_I); Anda, D [Anda, Dóra (Mikrobiológia), author] Doctoral School of Biology (ELTE / ELU FoS); Makk, J [Makk, Judit (Mikrobiológia), author] Department of Microbiology (ELTE / ELU FoS / Bio_I); Krett, G [Krett, Gergely (Mikrobiológia), author] Doctoral School of Biology (ELTE / ELU FoS); Dobosy, P [Dobosy, Péter (Környezetkémia), author] Danube Research Institute (CER); Büki, G; Erőss, A [Erőss, Anita (Hidrogeológia), author] Department of Physical and Applied Geology (ELTE / ELU FoS); Mádl-Szőnyi, J [Mádlné Szőnyi, Judit (Hidrogeológia), author] Department of Physical and Applied Geology (ELTE / ELU FoS)

English Article (Journal Article) Scientific
Published: JOURNAL OF BASIC MICROBIOLOGY 0233-111X 1521-4028 58 (11) pp. 928-937 2018
  • SJR Scopus - Applied Microbiology and Biotechnology: Q2
Identifiers
Subjects:
  • Basic medicine
  • Biological sciences
  • Other medical sciences
  • Clinical medicine
The Buda Thermal Karst System (BTKS) is an extensive active hypogenic cave system located beneath the residential area of the Hungarian capital. At the river Danube, several thermal springs discharge forming spring caves. To reveal and compare the morphological structure and prokaryotic diversity of reddish-brown biofilms developed on the carbonate rock surfaces of the springs, scanning electron microscopy (SEM), and molecular cloning were applied. Microbial networks formed by filamentous bacteria and other cells with mineral crystals embedded in extracellular polymeric substances were observed in the SEM images. Biofilms were dominated by prokaryotes belonging to phyla Proteobacteria, Chloroflexi and Nitrospirae (Bacteria) and Thaumarchaeota (Archaea) but their abundance showed differences according to the type of the host rock, geographic distance, and different water exchange. In addition, representatives of phyla Acidobacteria, Actinobacteria, Caldithrix, Cyanobacteria, Firmicutes Gemmatimonadetes, and several candidate divisions of Bacteria as well as Crenarchaeota and Euryarchaeota were detected in sample-dependent higher abundance. The results indicate that thermophilic, anaerobic sulfur-, sulfate-, nitrate-, and iron(III)-reducing chemoorganotrophic as well as sulfur-, ammonia-, and nitrite-oxidizing chemolithotrophic prokaryotes can interact in the studied biofilms adapted to the unique and extreme circumstances (e.g., aphotic and nearly anoxic conditions, oligotrophy, and radionuclide accumulation) in the thermal karst springs.
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2023-02-03 07:44