Az orvos-, egészségtudományi- és gyógyszerészképzés tudományos műhelyeinek fejlesztése(EFOP-3.6.3-VEKOP-16-2017-00009)
Támogató: EFOP-VEKOP
Chronic periodontitis is a bacterial infection associated with dentally adherent biofilm
(plaque) accumulation and age-related comorbidities. The disease begins as an inflammatory
exudate from gingival margins, gingival crevicular fluid (GCF) in response to biofilm
lysine. After a week of experimental gingivitis (no oral hygiene), biofilm lysine
concentration was linearly related to biofilm accumulation (plaque index) but to GCF
as an arch-shaped double curve which separated 9 strong from 6 weak GCF responders
(hosts). Host DNA was examined for single nucleotide polymorphisms (SNPs) of alleles
reported in 7 periodontitis-associated genes. Across all 15 hosts, an adenine SNP
(A) at IL1B-511 (rs16944), was significant for strong GCF (Fisher’s exact test, p
< 0.05), and a thymidine SNP (T) at IL1B+3954 (rs1143634) for weak GCF provided 2
hosts possessing IL6-1363(T), rs2069827, were included. The phenotype of IL1B+3954(T)
was converted from weak to strong in one host, and of the non-T allele from strong
to weak in the other (specific epistasis, Fisher’s exact test, p < 0.01). Together
with homozygous alternate or reference SNPs at IL10-1082 or CD14-260 in 4 hosts, all
hosts were identified as strong or weak GCF responders. The GCF response is therefore
a strong or weak genetic trait that indicates strong or weak innate immunity in EG
and controllable or uncontrollable periodontal disease, dental implant survival and
late-life comorbidities.
