https://m2.mtmt.hu/ eng 2026-05-04 10:19 JournalArticle 34141758 APPROVED true Department of Biochemistry, Institute of Biology, ELTE Eötvös Loránd University, Budapest, H-1117, Hungary ELTE—Functional Nucleic Acid Motifs Research Group, Department of Biochemistry, Institute of Biology, ELTE Eötvös Loránd University, Budapest, H-1117, Hungary MTA-ELTE Lendület Ion Mobility Mass Spectrometry Research Group, Department of Analytical Chemistry, Institute of Chemistry, ELTE Eötvös Loránd University, Budapest, H-1117, Hungary Doctoral School of Molecular Cell and Immune Biology, University of Debrecen, Egyetem tér 1, Debrecen, H-4032, Hungary Department of Biophysics and Cell Biology, Faculty of Medicine, University of Debrecen, Egyetem tér 1, Debrecen, H-4032, Hungary ELTE NAP Neuroimmunology Research Group, Department of Biochemistry, Institute of Biology, ELTE Eötvös Loránd University, Budapest, H-1117, Hungary Export Date: 10 October 2023 Correspondence Address: Kardos, J.; Department of Biochemistry, Hungary; email: kardos@elte.hu 0 false 2026-02-23T14:39:43.664+0000 2026-02-18T15:47:37.636+0000 2023-09-15T12:38:20.140+0000 true 10071503 Szabó Regina /api/admin/10071503 Admin Regina Szabó (ELTE TTK admin 5) true 2026-02-23T14:31:16.383+0000 true false true 24 24 /api/publicationtype/24 PublicationType Journal Article 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType Journal Article 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType Article (Journal Article) 101 true 10000059 true 1 /api/category/1 Category Scientific true 1 Murvai, Nikoletta true true /api/journal/10003252 REVIEWED INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 1661-6596 1422-0067 true false 10003252 false 10003252 true 1661-6596 1422-0067 Journal FOREIGN 24 17 13403 13403 2023 The aggregation and amyloid formation of α-synuclein is associated with Parkinson’s disease and other synucleinopathies. In its native, monomeric form α-synuclein is an intrinsically disordered protein represented by highly dynamic conformational ensembles. Inhibition of α-synuclein aggregation using small molecules, peptides, or proteins has been at the center of interest in recent years. Our aim was to explore the effects of cross-linking on the structure and aggregation/amyloid formation properties of α-synuclein. Comparative analysis of available high-resolution amyloid structures and representative structural models and MD trajectory of monomeric α-synuclein revealed that potential cross-links in the monomeric protein are mostly incompatible with the amyloid forms and thus might inhibit fibrillation. Monomeric α-synuclein has been intramolecularly chemically cross-linked under various conditions using different cross-linkers. We determined the location of cross-links and their frequency using mass spectrometry and found that most of them cannot be realized in the amyloid structures. The inhibitory potential of cross-linked proteins has been experimentally investigated using various methods, including thioflavin-T fluorescence and transmission electron microscopy. We found that conformational constraints applied by cross-linking fully blocked α-synuclein amyloid formation. Moreover, DTSSP-cross-linked molecules exhibited an inhibitory effect on the aggregation of unmodified α-synuclein as well. true 2023 true true true false false GOLD 2026-02-23 true https://doi.org/10.3390/ijms241713403 1 0 1 1 0 1 1 1 1 1 1 1 1 1 0 0 1 1 1 1 0 2 2 Folyóiratcikk Journal Article 24 1 Könyvrészlet Chapter in Book 25 0 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2024 0 1 1 1 1 D1 false 10079791,10020440,10060353 false false false 2023-09-14T13:04:05.776+0000 Biofizikai és Sejtbiológiai Intézet (DE / ÁOK); Biokémiai Tanszék (ELTE / TTK / Bio_I); MTA-ELTE Lendület Ionmobilitás-tömegspektrometr... (ELTE / TTK / KI); MTA-ELTE-NAP B Neuroimmunológiai Kutatócsoport (ELTE / TTK / Bio_I); Molekuláris Sejt- és Immunbiológia Doktori Iskola (DE / ODT) 5 31 5 5 0 true Language 10002 /api/language/10002 English Angol English true 2 true PersonAuthorship 109914765 /api/authorship/109914765 Murvai, Nikoletta [Murvai, Nikoletta (Molekuláris biológia), author] Department of Biochemistry (ELTE / ELU FoS / Bio_I) 1 0.2 true false false Author 10060353 /api/author/10060353 Nikoletta Murvai (Molekuláris biológia) Murvai Nikoletta true 10060353 true Murvai Nikoletta true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 109914766 /api/authorship/109914766 Gellen, Gabriella [Gellén, Gabriella (Proteomika), author] Department of Biophysics and Cell Biology (UD); Doctoral School of Molecular Cellular and Immun... (UD / ODT); MTA-ELTE Lendület Ion Mobility Mass Spectrometr... (ELTE / ELU FoS / IC) 2 0.2 false false false Author 10079791 /api/author/10079791 Gabriella Gellén (Proteomika) Gellén Gabriella true true Gellen Gabriella true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 109914767 /api/authorship/109914767 Micsonai, András [Micsonai, András (biokémia), author] Department of Biochemistry (ELTE / ELU FoS / Bio_I); MTA-ELTE-NAP B Neuroimmunology Research Group (ELTE / ELU FoS / Bio_I) 3 0.2 false false false Author 10020440 /api/author/10020440 András Micsonai (biokémia) Micsonai András true 10020440 true Micsonai András true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 109914768 /api/authorship/109914768 Schlosser, Gitta [Schlosser, Gitta (Tömegspektrometria), author] MTA-ELTE Lendület Ion Mobility Mass Spectrometr... (ELTE / ELU FoS / IC) 4 0.2 false false false Author 10002259 /api/author/10002259 Gitta Schlosser (Tömegspektrometria) Schlosser Gitta true 10002259 true Schlosser Gitta true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 109914769 /api/authorship/109914769 Kardos, József ✉ [Kardos, József (Biológia), author] Department of Biochemistry (ELTE / ELU FoS / Bio_I); MTA-ELTE-NAP B Neuroimmunology Research Group (ELTE / ELU FoS / Bio_I) 5 0.2 false true true Author 10014881 /api/author/10014881 József Kardos (Biológia) Kardos József true 10014881 true Kardos József true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PublicationIdentifier 24376921 /api/publicationidentifier/24376921 DOI: 10.3390/ijms241713403 PlainSource 6 /api/publicationsource/6 DOI PublicationSourceType 10001 /api/publicationsourcetype/10001 DOI true true true DOI DOI https://doi.org/@@@ true true 6 true GOLD true IDENTICAL 10.3390/ijms241713403 https://doi.org/10.3390/ijms241713403 false true PublicationIdentifier 24379960 /api/publicationidentifier/24379960 WoS: 001061219600001 PlainSource 1 /api/publicationsource/1 WoS PublicationSourceType 10003 /api/publicationsourcetype/10003 Indexelő adatbázis false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true IDENTICAL 001061219600001 https://www.webofscience.com/wos/woscc/full-record/001061219600001 false true PublicationIdentifier 24377015 /api/publicationidentifier/24377015 Scopus: 85170231963 PlainSource 3 /api/publicationsource/3 Scopus PublicationSourceType 10003 /api/publicationsourcetype/10003 Indexelő adatbázis false true true Scopus Scopus http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@ true true 3 true IDENTICAL 85170231963 http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85170231963 false true PublicationIdentifier 31474632 /api/publicationidentifier/31474632 PubMed: 37686208 PlainSource 17 /api/publicationsource/17 PubMed PublicationSourceType 10003 /api/publicationsourcetype/10003 Indexelő adatbázis false true true PubMed PubMed http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=@@@&dopt=Abstract true true 17 true IDENTICAL 37686208 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=37686208&dopt=Abstract false true PublicationIdentifier 24376922 /api/publicationidentifier/24376922 Other URL: https://www.mdpi.com/1422-0067/24/17/13403 PlainSource 40 /api/publicationsource/40 Other URL PublicationSourceType 10006 /api/publicationsourcetype/10006 Link true true true Egyéb URL Other URL @@@ true true 40 true https://www.mdpi.com/1422-0067/24/17/13403 https://www.mdpi.com/1422-0067/24/17/13403 false true SjrRating 11388415 /api/sjrrating/11388415 sjr:D1 (2023) Scopus - Inorganic Chemistry INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 1661-6596 1422-0067 7 0.1 journal RatingType 10002 /api/ratingtype/10002 sjr sjr true true ClassificationExternal 1604 /api/classificationexternal/1604 Scopus - Inorganic Chemistry true 1604 true D1 DIRECT true true Reference 45069161 /api/reference/45069161 1. 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(2023) INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 1661-6596 1422-0067 24 17<div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10060353"><a href="/gui2/?type=authors&mode=browse&sel=10060353" target="_blank">Murvai Nikoletta (<span class="authorship-author-name">Murvai Nikoletta</span> <span class="authorAux-mtmt"> Molekuláris biológia</span>) </a> </span> <span class="author-affil"><span title="Eötvös Loránd University">ELTE</span>/<span title="Faculty of Science">ELU FoS</span>/<span title="Institute of Biology">Bio_I</span>/Department of Biochemistry</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10079791"><a href="/gui2/?type=authors&mode=browse&sel=10079791" target="_blank">Gellen Gabriella (<span class="authorship-author-name">Gellén Gabriella</span> <span class="authorAux-mtmt"> Proteomika</span>) </a> </span> <span class="author-affil"><span title="University of Debrecen">UD</span>/<span title="Faculty of Medicine">Faculty of Medicine</span>/Department of Biophysics and Cell Biology; <span title="University of Debrecen">UD</span>/<span title="Orvostudományi Doktori Tanács">ODT</span>/Doctoral School of Molecular Cellular and Immune Biology; <span title="Eötvös Loránd University">ELTE</span>/<span title="Faculty of Science">ELU FoS</span>/<span title="Insititute of Chemistry">IC</span>/MTA-ELTE Lendület Ion Mobility Mass Spectrometry Research Group</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10020440"><a href="/gui2/?type=authors&mode=browse&sel=10020440" target="_blank">Micsonai András (<span class="authorship-author-name">Micsonai András</span> <span class="authorAux-mtmt"> biokémia</span>) </a> </span> <span class="author-affil"><span title="Eötvös Loránd University">ELTE</span>/<span title="Faculty of Science">ELU FoS</span>/<span title="Institute of Biology">Bio_I</span>/Department of Biochemistry; <span title="Eötvös Loránd University">ELTE</span>/<span title="Faculty of Science">ELU FoS</span>/<span title="Institute of Biology">Bio_I</span>/MTA-ELTE-NAP B Neuroimmunology Research Group</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10002259"><a href="/gui2/?type=authors&mode=browse&sel=10002259" target="_blank">Schlosser Gitta (<span class="authorship-author-name">Schlosser Gitta</span> <span class="authorAux-mtmt"> Tömegspektrometria</span>) </a> </span> <span class="author-affil"><span title="Eötvös Loránd University">ELTE</span>/<span title="Faculty of Science">ELU FoS</span>/<span title="Insititute of Chemistry">IC</span>/MTA-ELTE Lendület Ion Mobility Mass Spectrometry Research Group</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10014881"><a href="/gui2/?type=authors&mode=browse&sel=10014881" target="_blank">Kardos József ✉ (<span class="authorship-author-name">Kardos József</span> <span class="authorAux-mtmt"> Biológia</span>) </a> </span> <span class="author-affil"><span title="Eötvös Loránd University">ELTE</span>/<span title="Faculty of Science">ELU FoS</span>/<span title="Institute of Biology">Bio_I</span>/Department of Biochemistry; <span title="Eötvös Loránd University">ELTE</span>/<span title="Faculty of Science">ELU FoS</span>/<span title="Institute of Biology">Bio_I</span>/MTA-ELTE-NAP B Neuroimmunology Research Group</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;34141758" target="_blank">Cross-Linked α-Synuclein as Inhibitor of Amyloid Formation</a></div> <div> <span class="journal-title">INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/1661-6596">1661-6596</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1422-0067">1422-0067</a>)</span>: <span class="journal-volume">24</span> <span class="journal-issue">17</span> <span class="page"> Paper 13403. </span> <span class="year">(2023)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Language: English | </span> <span class="identifiers"> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="10.3390/ijms241713403" target="_blank" href="https://doi.org/10.3390/ijms241713403"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="001061219600001" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/001061219600001"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="85170231963" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85170231963"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="37686208" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=37686208&dopt=Abstract"> PubMed </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:black" title="https://www.mdpi.com/1422-0067/24/17/13403" target="_blank" href="https://www.mdpi.com/1422-0067/24/17/13403"> Other URL </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Journal subject: Scopus - Inorganic Chemistry&nbsp;&nbsp;&nbsp;Rank:&nbsp;D1</div> <div class="journal-subject">Journal subject: Scopus - Organic Chemistry&nbsp;&nbsp;&nbsp;Rank:&nbsp;D1</div> <div class="journal-subject">Journal subject: Scopus - Spectroscopy&nbsp;&nbsp;&nbsp;Rank:&nbsp;D1</div> <div class="journal-subject">Journal subject: Scopus - Computer Science Applications&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q1</div> <div class="journal-subject">Journal subject: Scopus - Medicine (miscellaneous)&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q1</div> <div class="journal-subject">Journal subject: Scopus - Physical and Theoretical Chemistry&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q1</div> <div class="journal-subject">Journal subject: Scopus - Catalysis&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q2</div> <div class="journal-subject">Journal subject: Scopus - Molecular Biology&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q2</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="" class="citingPub-count">Citing papers: 1</span> | Independent citation: 1 | Self citation: 0 | Unknown citation: 0 | Number of citations in WoS: 1 | Number of citations in Scopus:&nbsp;1 | WoS/Scopus assigned:&nbsp;1 | Number of citations with DOI:&nbsp;1 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;34141758&sort=publishedYear,desc&sort=title"> Number of cited publications: 2 </a> </div> <div class="mtid"><span class="long-pub-mtid">Publication: 34141758</span> | <span class="status-data status-APPROVED"> Published </span> Core Citing | <span class="type-subtype">Journal Article ( Article ) </span> | <span class="pub-category">Scientific</span> | <span class="publication-sourceOfData">kézi felvitel</span> </div> <div class="lastModified">Last Modified: 2026.02.18. 16:47 Regina Szabó (ELTE TTK admin 5) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Comments</u>: Department of Biochemistry, Institute of Biology, ELTE Eötvös Loránd University, Budapest, H-1117, Hungary ELTE—Functional Nucleic Acid Motifs Research Group, Department of Biochemistry, Institute of Biology, ELTE Eötvös Loránd University, Budapest, H-1117, Hungary MTA-ELTE Lendület Ion Mobility Mass Spectrometry Research Group, Department of Anal...</pre> </div></div>