Átfogó fejlesztések a Pécsi Tudományegyetemen az intelligens szakosodás megvalósítása
érdekében(EFOP-3.6.1-16-2016-00004) Támogató: EFOP
The global prevalence of insulin resistance (IR) is increasing continuously, influencing
metabolic parameters and fertility. The metabolic changes due to IR can alter the
molecular composition of plasma and other body fluids. Follicular fluid (FF) is derived
mainly from plasma, and it is a critical microenvironment for the developing oocytes.
It contains various metabolites and amino acids, and the quality of the oocytes is
linked at least partially to amino acid metabolism. Our goal was to quantitatively
determine the amino acid (AA) profile of FF in IVF patients and to compare IR and
non-insulin resistance (NIR) groups to investigate the AA changes in their FF. Using
UHPLC-based methods, we quantified the main 20 amino acids from human FF samples in
the IR and NIR groups. Several amino acids (aspartate, glycine, glutamate, and cysteine)
differed significantly (p < 0.05 or less) between the two groups. The most significant
alterations between the IR and NIR groups were related to the glutathione metabolic
pathway involving glycine, serine, and threonine. Since insulin resistance alters
the amino acid composition of the FF, the oocytes may undergo metabolism-induced changes
resulting in poor oocyte quality and less fertility in the insulin resistance groups.
