https://m2.mtmt.hu/ hun 2026-05-26 09:49 JournalArticle 34064647 VALIDATED true Department of Biochemistry, Semmelweis University, Tuzolto u. 37-47, Budapest, 1094, Hungary Department of Physiology, Semmelweis University, Tuzolto u. 37-47, Budapest, 1094, Hungary ELKH-SE Ion Channel Research Group, Semmelweis University, Tuzolto u. 37-47, Budapest, 1094, Hungary HCEMM-SE Molecular Channelopathies Research Group, Semmelweis University, Tuzolto u. 37-47, Budapest, 1094, Hungary Export Date: 4 September 2023 Correspondence Address: Szollosi, A.; Department of Biochemistry, Tuzolto u. 37-47, Hungary; email: szollo5@gmail.com Chemicals/CAS: adenosine diphosphate ribose, 20762-30-5; calcium, 7440-70-2, 14092-94-5; Adenosine Diphosphate Ribose; Calcium; Cations; Lipid Bilayers; TRPM Cation Channels Funding details: 739593 Funding details: Nemzeti Kutatási, Fejlesztési és Innovaciós Alap, NKFIA, KKP 144199 Funding text 1: This work was supported by Semmelweis Science and Innovation Fund STIA_18_M; EU Horizon 2020 Research and Innovation Program grant 739593 and National Research, Development and Innovation Fund grant KKP 144199. We thank László Csanády for funding and thorough reading and comments on the manuscript. We thank Balázs Tóth for help with poly- l -lysine measurements and stimulating discussions. 0 false 2026-05-23T02:22:01.733+0000 2026-04-22T06:53:21.995+0000 2023-07-17T08:44:31.802+0000 true 10020191 Szöllősi András /api/author/10020191 Author Szöllősi András (Biokémia) true 10020191 2026-04-30T09:09:13.894+0000 2023-07-25T10:08:33.682+0000 true 10062250 Molnár-Taga Márta /api/admin/10062250 Admin Molnár-Taga Márta (SE 4-es admin) true 10062250 true false true 24 24 /api/publicationtype/24 PublicationType Folyóiratcikk 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType Folyóiratcikk 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType Szakcikk (Folyóiratcikk) 101 true 10000059 true 1 /api/category/1 Category Tudományos true 1 Szollosi, Andras true true /api/journal/10019966 REVIEWED SCIENTIFIC REPORTS 2045-2322 true false 10019966 false 10019966 true 2045-2322 Journal FOREIGN 13 1 11471 11471 10 2023 Transient receptor potential melastatin 2 (TRPM2) cation channel activity is required for insulin secretion, immune cell activation and body heat control. Channel activation upon oxidative stress is involved in the pathology of stroke and neurodegenerative disorders. Cytosolic Ca 2+ , ADP-ribose (ADPR) and phosphatidylinositol-4,5-bisphosphate (PIP 2 ) are the obligate activators of the channel. Several TRPM2 cryo-EM structures have been resolved to date, yet functionality of the purified protein has not been tested. Here we reconstituted overexpressed and purified TRPM2 from Nematostella vectensis (nvTRPM2) into lipid bilayers and found that the protein is fully functional. Consistent with the observations in native membranes, nvTRPM2 in lipid bilayers is co-activated by cytosolic Ca 2+ and either ADPR or ADPR-2′-phosphate (ADPRP). The physiological metabolite ADPRP has a higher apparent affinity than ADPR. In lipid bilayers nvTRPM2 displays a large linear unitary conductance, its open probability (P o ) shows little voltage dependence and is stable over several minutes. P o is high without addition of exogenous PIP 2 , but is largely blunted by treatment with poly- l -Lysine, a polycation that masks PIP 2 headgroups. These results indicate that PIP 2 or some other activating phosphoinositol lipid co-purifies with nvTRPM2, suggesting a high PIP 2 binding affinity of nvTRPM2 under physiological conditions. Funding 2029213 /api/funding/2029213 (STIA_18_M) false true Funding 2029214 /api/funding/2029214 Establishing the Hungarian Center of Excellence for Molecular Medicine in partnership with EMBL(739593) Támogató: Horizon 2020 false true Funding 2029215 /api/funding/2029215 (KKP 144199) false true true true 2023 true true true false true false GOLD 2026-05-23 false http://www.nature.com/srep/index.html 2 0 2 1 1 2 2 1 2 2 2 1 1 1 1 0 2 1 2 1 0 8 8 Folyóiratcikk Journal Article 24 2 Könyvrészlet Chapter in Book 25 0 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2025 0 2 1 2 1 D1 A nemzetközi false 10034375,10020191 false false false 2023-07-17T14:00:42.495+0000 Biokémiai Tanszék (SE / AOK / I / BMBI); Biokémiai és Molekuláris Biológiai Intézet (SE / AOK / I); ELKH-SE Ioncsatorna Kutatócsoport (SE / AOK / I / BMBI / BT); HCEMM-SE Molekuláris Csatornabetegségek Kutatóc... (SE / AOK / I / BMBI / BT); MTA-SE Lendület Ioncsatorna Kutatócsoport (SE / AOK / I / BMBI / BT); Élettani Intézet (SE / AOK / I) 2 18 6 2 0 true Language 10002 /api/language/10002 Angol Angol English true 2 true PersonAuthorship 109293544 /api/authorship/109293544 Szollosi, Andras ✉ [Szöllősi, András (Biokémia), szerző] MTA-SE Lendület Ioncsatorna Kutatócsoport (SE / AOK / I / BMBI / BT); Biokémiai és Molekuláris Biológiai Intézet (SE / AOK / I); Biokémiai Tanszék (SE / AOK / I / BMBI); ELKH-SE Ioncsatorna Kutatócsoport (SE / AOK / I / BMBI / BT); HCEMM-SE Molekuláris Csatornabetegségek Kutatóc... 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(2023) SCIENTIFIC REPORTS 2045-2322 13 1<div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10020191"><a href="/gui2/?type=authors&mode=browse&sel=10020191" target="_blank">Szollosi Andras ✉ (<span class="authorship-author-name">Szöllősi András</span> <span class="authorAux-mtmt"> Biokémia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/<span title="Biokémiai és Molekuláris Biológiai Intézet">BMBI</span>/<span title="Biokémiai Tanszék">BT</span>/MTA-SE Lendület Ioncsatorna Kutatócsoport; <span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Biokémiai és Molekuláris Biológiai Intézet; <span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/<span title="Biokémiai és Molekuláris Biológiai Intézet">BMBI</span>/Biokémiai Tanszék; <span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/<span title="Biokémiai és Molekuláris Biológiai Intézet">BMBI</span>/<span title="Biokémiai Tanszék">BT</span>/ELKH-SE Ioncsatorna Kutatócsoport; <span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/<span title="Biokémiai és Molekuláris Biológiai Intézet">BMBI</span>/<span title="Biokémiai Tanszék">BT</span>/HCEMM-SE Molekuláris Csatornabetegségek Kutatócsoport</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10034375"><a href="/gui2/?type=authors&mode=browse&sel=10034375" target="_blank">Almássy János (<span class="authorship-author-name">Almássy János</span> <span class="authorAux-mtmt"> sejtélettan, incsatornák élettana, intracellulá...</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Élettani Intézet</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;34064647" target="_blank">Functional characterization of the transient receptor potential melastatin 2 (TRPM2) cation channel from Nematostella vectensis reconstituted into lipid bilayer</a></div> <div> <span class="journal-title">SCIENTIFIC REPORTS</span> <span class="journal-issn">( <a target="_blank" href="https://portal.issn.org/resource/ISSN/2045-2322">2045-2322</a>)</span>: <span class="journal-volume">13</span> <span class="journal-issue">1</span> <span class="page"> Paper 11471. 10 p. </span> <span class="year">(2023)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="10.1038/s41598-023-38640-6" target="_blank" href="https://doi.org/10.1038/s41598-023-38640-6"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="001055239000013" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/001055239000013"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="85164760224" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85164760224"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="37454209" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=37454209&dopt=Abstract"> PubMed </a> </span> <span class="id identifier oa_NONE" title=" Nincs "> <a style="color:blue" title="https://www.scopus.com/pages/publications/85164760224?origin=resultslist" target="_blank" href="https://www.scopus.com/pages/publications/85164760224?origin=resultslist"> Egyéb URL </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Folyóirat szakterülete: Scopus - Multidisciplinary&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> <div class="journal-subject">Szociológiai Tudományos Bizottság IXGJO SZTB [1901-]&nbsp;&nbsp;&nbsp;A nemzetközi</div> <div class="journal-subject">Regionális Tudományok Bizottsága IXGJO RTB [1901-]&nbsp;&nbsp;&nbsp;B nemzetközi</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 2</span> | Független: 1 | Függő: 1 | Nem jelölt: 0 | WoS jelölt: 1 | Scopus jelölt:&nbsp;2 | WoS/Scopus jelölt:&nbsp;2 | DOI jelölt:&nbsp;2 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;34064647&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 8 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 34064647</span> | <span class="status-data status-VALIDATED"> Egyeztetett </span> Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">kézi felvitel</span> </div> <div class="funder"> (STIA_18_M), HCEMM(739593) Támogató: Horizon 2020, (KKP 144199) </div> <div class="lastModified">Utolsó módosítás: 2026.04.22. 08:53 Kajtár Virág Anna (SE_AOK_Biokemia_Admin5_KV, admin) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Megjegyzés</u>: Department of Biochemistry, Semmelweis University, Tuzolto u. 37-47, Budapest, 1094, Hungary Department of Physiology, Semmelweis University, Tuzolto u. 37-47, Budapest, 1094, Hungary ELKH-SE Ion Channel Research Group, Semmelweis University, Tuzolto u. 37-47, Budapest, 1094, Hungary HCEMM-SE Molecular Channelopathies Resea...</pre> </div></div>