{ "labelLang" : "hun", "responseDate" : "2024-03-28 10:24", "content" : { "otype" : "JournalArticle", "mtid" : 3384042, "status" : "VALIDATED", "published" : true, "comment" : "Department of Applied Biotechnology and Food Science, Budapest University of Technology and Economics, Műegyetem rkp. 3, Budapest, H-1111, Hungary \n Biotechnology Development Department, Gedeon Richter Plc., Gyömrői út 19-21, Budapest, H-1103, Hungary \n Cited By :3 \n Export Date: 14 September 2021 \n CODEN: JPBAD \n Correspondence Address: Kozma, B.; Department of Applied Biotechnology and Food Science, Műegyetem rkp. 3, H-1111 Budapest, Hungary; email: bkozma@mail.bme.hu \n Chemicals/CAS: glucose, 50-99-7, 84778-64-3; Antibodies, Monoclonal; Glucose \n Funding details: Richter Gedeon Talentum Alapítvány \n Funding text 1: This work was partially funded by Gedeon Richter Talentum Foundation (1103 Budapest, Gyömrői út 19-21, Hungary). \n Funding text 2: This work was performed in the frame of FIEK_16-1-2016-0007 project, implemented with the support provided from the National Research, Development and Innovation Fund of Hungary , financed under the FIEK_16 funding scheme.", "unhandledTickets" : 0, "oldTimestamp" : "2018-10-02T08:54:25.000+0000", "deleted" : false, "oldId" : 3384042, "lastRefresh" : "2024-02-19T07:43:42.784+0000", "lastModified" : "2023-07-26T06:53:19.762+0000", "created" : "2018-06-10T05:45:41.000+0000", "creator" : { "otype" : "Author", "mtid" : 10031127, "link" : "/api/author/10031127", "label" : "Gergely Szilveszter (infravörös spektroszkópia)", "familyName" : "Gergely", "givenName" : "Szilveszter", "published" : true, "oldId" : 10031127, "snippet" : true }, "lastDuplumSearch" : "2023-03-07T10:01:49.375+0000", "adminApproved" : "2018-07-02T13:20:00.000+0000", "adminApprover" : { "otype" : "Admin", "mtid" : 10039288, "link" : "/api/admin/10039288", "label" : "Szmolyán Mária (BME 4 admin - INAKTÍV)", "familyName" : "Szmolyán", "givenName" : "Mária", "published" : true, "oldId" : 10039288, "snippet" : true }, "validated" : "2018-07-02T13:20:00.000+0000", "validator" : { "otype" : "Admin", "mtid" : 10039288, "link" : "/api/admin/10039288", "label" : "Szmolyán Mária (BME 4 admin - INAKTÍV)", "familyName" : "Szmolyán", "givenName" : "Mária", "published" : true, "oldId" : 10039288, "snippet" : true }, "core" : true, "citation" : true, "publicationPending" : false, "type" : { "otype" : "PublicationType", "mtid" : 24, "link" : "/api/publicationtype/24", "label" : "Folyóiratcikk", "code" : 24, "otypeName" : "JournalArticle", "listPosition" : 1, "published" : true, "oldId" : 24, "snippet" : true }, "subType" : { "otype" : "SubType", "mtid" : 10000059, "link" : "/api/subtype/10000059", "label" : "Szakcikk (Folyóiratcikk)", "name" : "Szakcikk", "nameEng" : "Article", "docType" : { "otype" : "PublicationType", "mtid" : 24, "link" : "/api/publicationtype/24", "label" : "Folyóiratcikk", "code" : 24, "otypeName" : "JournalArticle", "listPosition" : 1, "published" : true, "oldId" : 24, "snippet" : true }, "listPosition" : 101, "published" : true, "oldId" : 10000059, "snippet" : true }, "category" : { "otype" : "Category", "mtid" : 1, "link" : "/api/category/1", "label" : "Tudományos", "published" : true, "oldId" : 1, "snippet" : true }, "languages" : [ { "otype" : "Language", "mtid" : 10002, "link" : "/api/language/10002", "label" : "Angol", "name" : "Angol", "nameEng" : "English", "published" : true, "oldId" : 2, "snippet" : true } ], "firstAuthor" : "Kozma, Bence", "authorships" : [ { "otype" : "PersonAuthorship", "mtid" : 10183406, "link" : "/api/authorship/10183406", "label" : "Kozma, Bence [Kozma, Bence (biotechnológia), szerző] Richter Gedeon Nyrt.", "listPosition" : 1, "share" : 0.33333334, "first" : true, "last" : false, "author" : { "otype" : "Author", "mtid" : 10049595, "link" : "/api/author/10049595", "label" : "Kozma Bence (biotechnológia)", "familyName" : "Kozma", "givenName" : "Bence", "published" : true, "oldId" : 10049595, "snippet" : true }, "familyName" : "Kozma", "givenName" : "Bence", "authorTyped" : true, "editorTyped" : false, "otherTyped" : false, "type" : { "otype" : "AuthorshipType", "mtid" : 1, "link" : "/api/authorshiptype/1", "label" : "Szerző", "code" : 0, "published" : true, "oldId" : 0, "snippet" : true }, "published" : false, "oldId" : 28370804, "snippet" : true }, { "otype" : "PersonAuthorship", "mtid" : 10183407, "link" : "/api/authorship/10183407", "label" : "Salgó, András [Salgó, András (Élelmiszertudomán...), szerző] Alkalmazott Biotechnológia és Élelmiszertudomán... (BME / VBK)", "listPosition" : 2, "share" : 0.33333334, "first" : false, "last" : false, "author" : { "otype" : "Author", "mtid" : 10005745, "link" : "/api/author/10005745", "label" : "Salgó András (Élelmiszertudomány, közeli infravörös spektroszkópia)", "familyName" : "Salgó", "givenName" : "András", "published" : true, "oldId" : 10005745, "snippet" : true }, "familyName" : "Salgó", "givenName" : "András", "authorTyped" : true, "editorTyped" : false, "otherTyped" : false, "type" : { "otype" : "AuthorshipType", "mtid" : 1, "link" : "/api/authorshiptype/1", "label" : "Szerző", "code" : 0, "published" : true, "oldId" : 0, "snippet" : true }, "published" : false, "oldId" : 28370803, "snippet" : true }, { "otype" : "PersonAuthorship", "mtid" : 10183408, "link" : "/api/authorship/10183408", "label" : "Gergely, Szilveszter [Gergely, Szilveszter (infravörös spektr...), szerző] Alkalmazott Biotechnológia és Élelmiszertudomán... (BME / VBK)", "listPosition" : 3, "share" : 0.33333334, "first" : false, "last" : true, "author" : { "otype" : "Author", "mtid" : 10031127, "link" : "/api/author/10031127", "label" : "Gergely Szilveszter (infravörös spektroszkópia)", "familyName" : "Gergely", "givenName" : "Szilveszter", "published" : true, "oldId" : 10031127, "snippet" : true }, "familyName" : "Gergely", "givenName" : "Szilveszter", "authorTyped" : true, "editorTyped" : false, "otherTyped" : false, "type" : { "otype" : "AuthorshipType", "mtid" : 1, "link" : "/api/authorshiptype/1", "label" : "Szerző", "code" : 0, "published" : true, "oldId" : 0, "snippet" : true }, "published" : false, "oldId" : 28370802, "snippet" : true } ], "title" : "Comparison of multivariate data analysis techniques to improve glucose concentration prediction in mammalian cell cultivations by Raman spectroscopy", "identifiers" : [ { "otype" : "PublicationIdentifier", "mtid" : 1304565, "link" : "/api/publicationidentifier/1304565", "label" : "DOI: 10.1016/j.jpba.2018.06.005", "source" : { "otype" : "PlainSource", "mtid" : 6, "link" : "/api/publicationsource/6", "label" : "DOI", "type" : { "otype" : "PublicationSourceType", "mtid" : 10001, "link" : "/api/publicationsourcetype/10001", "label" : "DOI", "mayHaveOa" : true, "published" : true, "snippet" : true }, "name" : "DOI", "nameEng" : "DOI", "linkPattern" : "https://doi.org/@@@", "publiclyVisible" : true, "published" : true, "oldId" : 6, "snippet" : true }, "oaType" : "PAY", "oaFree" : false, "validState" : "IDENTICAL", "idValue" : "10.1016/j.jpba.2018.06.005", "realUrl" : "https://doi.org/10.1016/j.jpba.2018.06.005", "published" : false, "oldId" : 1786747, "snippet" : true }, { "otype" : "PublicationIdentifier", "mtid" : 15453977, "link" : "/api/publicationidentifier/15453977", "label" : "WoS: 000441369500030", "source" : { "otype" : "PlainSource", "mtid" : 1, "link" : "/api/publicationsource/1", "label" : "WoS", "type" : { "otype" : "PublicationSourceType", "mtid" : 10003, "link" : "/api/publicationsourcetype/10003", "label" : "Indexelő adatbázis", "mayHaveOa" : false, "published" : true, "snippet" : true }, "name" : "WoS", "nameEng" : "WoS", "linkPattern" : "https://www.webofscience.com/wos/woscc/full-record/@@@", "publiclyVisible" : true, "published" : true, "oldId" : 1, "snippet" : true }, "validState" : "IDENTICAL", "idValue" : "000441369500030", "realUrl" : "https://www.webofscience.com/wos/woscc/full-record/000441369500030", "published" : false, "snippet" : true }, { "otype" : "PublicationIdentifier", "mtid" : 1304564, "link" : "/api/publicationidentifier/1304564", "label" : "Scopus: 85048595947", "source" : { "otype" : "PlainSource", "mtid" : 3, "link" : "/api/publicationsource/3", "label" : "Scopus", "type" : { "otype" : "PublicationSourceType", "mtid" : 10003, "link" : "/api/publicationsourcetype/10003", "label" : "Indexelő adatbázis", "mayHaveOa" : false, "published" : true, "snippet" : true }, "name" : "Scopus", "linkPattern" : "http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@", "publiclyVisible" : true, "published" : true, "oldId" : 3, "snippet" : true }, "oaFree" : false, "validState" : "IDENTICAL", "idValue" : "85048595947", "realUrl" : "http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85048595947", "published" : false, "oldId" : 1797483, "snippet" : true }, { "otype" : "PublicationIdentifier", "mtid" : 23198570, "link" : "/api/publicationidentifier/23198570", "label" : "PubMed: 29894949", "source" : { "otype" : "PlainSource", "mtid" : 17, "link" : "/api/publicationsource/17", "label" : "PubMed", "type" : { "otype" : "PublicationSourceType", "mtid" : 10003, "link" : "/api/publicationsourcetype/10003", "label" : "Indexelő adatbázis", "mayHaveOa" : false, "published" : true, "snippet" : true }, "name" : "PubMed", "nameEng" : "PubMed", "linkPattern" : "http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=@@@&dopt=Abstract", "publiclyVisible" : true, "published" : true, "oldId" : 17, "snippet" : true }, "validState" : "IDENTICAL", "idValue" : "29894949", "realUrl" : "http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=29894949&dopt=Abstract", "published" : false, "snippet" : true } ], "journal" : { "otype" : "Journal", "mtid" : 2766, "link" : "/api/journal/2766", "label" : "JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 0731-7085 1873-264X", "pIssn" : "0731-7085", "eIssn" : "1873-264X", "reviewType" : "REVIEWED", "noIF" : false, "sciIndexed" : true, "scopusIndexed" : true, "lang" : "FOREIGN", "hungarian" : false, "published" : true, "oldId" : 2766, "snippet" : true }, "volume" : "158", "firstPage" : "269", "lastPage" : "279", "firstPageOrInternalIdForSort" : "269", "pageLength" : 11, "publishedYear" : 2018, "abstractText" : "Abstract In-situ Raman spectroscopy is frequently applied to monitor and even control the glucose concentration of monoclonal antibody producing mammalian cell cultivations. Previous studies used the PLSR algorithm only, however other multivariate algorithms were applied successfully for different protein production processes. In this study, four mammalian cell cultivation runs were followed with Raman spectroscopy and the spectra were analysed quantitatively and qualitatively as well. The PCA analysis showed that one of the most dominant factors in the Raman spectra were the concentration of glucose, which strongly correlated with the score values of the eighth principal component. This observation further substantiated that Raman spectroscopy is an excellent tool for bioprocess monitoring and induced the test of the Multivariate Linear Regression (MLR), Principal Component Regression (PCR) and Partial Least Squares Regression (PLSR) algorithms, using the results of the PCA as one of the variable selection techniques, to determine the glucose concentration during cultivation. However, the novel variable selection technique of PCA correlation enhanced only the model accuracy when it was applied with MLR and only model robustness was increased when it was used with PCR and PLSR because the relatively strong Raman signal of glucose concentration seemed to be enough to build an accurate model on. Therefore, PLSR, the most advanced algorithm of the three, delivered the lowest 2.21 mM RMSEP but it was demonstrated that in certain cases PCR could also produce satisfactorily results.", "subjects" : [ { "otype" : "Classification", "mtid" : 12125, "link" : "/api/classification/12125", "label" : "Általános orvostudomány", "published" : true, "snippet" : true }, { "otype" : "Classification", "mtid" : 10293, "link" : "/api/classification/10293", "label" : "Kémiai tudományok", "published" : true, "snippet" : true }, { "otype" : "Classification", "mtid" : 12215, "link" : "/api/classification/12215", "label" : "Klinikai orvostan", "published" : true, "snippet" : true } ], "keywords" : [ { "otype" : "Keyword", "mtid" : 4855, "link" : "/api/keyword/4855", "label" : "GLUCOSE", "published" : true, "oldId" : 4855, "snippet" : true }, { "otype" : "Keyword", "mtid" : 5063, "link" : "/api/keyword/5063", "label" : "PCR", "published" : true, "oldId" : 5063, "snippet" : true }, { "otype" : "Keyword", "mtid" : 12359, "link" : "/api/keyword/12359", "label" : "MONITORING", "published" : true, "oldId" : 12359, "snippet" : true }, { "otype" : "Keyword", "mtid" : 17214, "link" : "/api/keyword/17214", "label" : "raman spectroscopy", "published" : true, "oldId" : 17214, "snippet" : true }, { "otype" : "Keyword", "mtid" : 1047639, "link" : "/api/keyword/1047639", "label" : "MLR", "published" : true, "oldId" : 1047639, "snippet" : true }, { "otype" : "Keyword", "mtid" : 1392703, "link" : "/api/keyword/1392703", "label" : "CHO", "published" : true, "oldId" : 1392703, "snippet" : true }, { "otype" : "Keyword", "mtid" : 1478979, "link" : "/api/keyword/1478979", "label" : "Mammalian cell cultivation", "published" : true, "oldId" : 1478979, "snippet" : true }, { "otype" : "Keyword", "mtid" : 1478980, "link" : "/api/keyword/1478980", "label" : "PLSR", "published" : true, "oldId" : 1478980, "snippet" : true } ], "digital" : null, "printed" : null, "sourceYear" : 2018, "packet" : "523841", "foreignEdition" : true, "foreignLanguage" : true, "fullPublication" : true, "conferencePublication" : false, "nationalOrigin" : true, "missingAuthor" : false, "oaType" : "PAY", "oaCheckDate" : "2024-02-19", "oaFree" : false, "citationCount" : 14, "citationCountUnpublished" : 0, "citationCountWoOther" : 14, "independentCitCountWoOther" : 13, "nationalOriginCitationCount" : 1, "foreignEditionCitationCount" : 14, "doiCitationCount" : 14, "wosCitationCount" : 14, "scopusCitationCount" : 7, "wosScopusCitationCount" : 14, "wosScopusCitationCountWoOther" : 14, "wosScopusIndependentCitationCount" : 13, "wosScopusIndependentCitationCountWoOther" : 13, "independentCitationCount" : 13, "selfCitationCount" : 1, "unhandledCitationCount" : 0, "citingPubCount" : 14, "independentCitingPubCount" : 13, "citingPubCountWoOther" : 14, "independentCitingPubCountWoOther" : 13, "unhandledCitingPubCount" : 0, "citedPubCount" : 2, "citedCount" : 2, "pubStats" : { "types" : [ { "type" : "Folyóiratcikk", "typeEng" : "Journal Article", "code" : 24, "count" : 14 }, { "type" : "Könyvrészlet", "typeEng" : "Chapter in Book", "code" : 25, "count" : 0 }, { "type" : "Könyv", "typeEng" : "Book", "code" : 23, "count" : 0 }, { "type" : "Egyéb konferenciaközlemény", "typeEng" : "Conference paper", "code" : 31, "count" : 0 }, { "type" : "Egyéb konferenciakötet", "typeEng" : "Conference proceedings", "code" : 32, "count" : 0 }, { "type" : "Oltalmi formák", "typeEng" : "Protection forms", "code" : 26, "count" : 0 }, { "type" : "Disszertáció", "typeEng" : "Thesis", "code" : 28, "count" : 0 }, { "type" : "Egyéb", "typeEng" : "Miscellaneous", "code" : 29, "count" : 0 }, { "type" : "Alkotás", "typeEng" : "Achievement", "code" : 22, "count" : 0 }, { "type" : "Kutatási adat", "typeEng" : "Research data", "code" : 33, "count" : 0 } ], "citationTypes" : [ { "type" : "Folyóiratcikk", "typeEng" : "Journal Article", "code" : 24, "countUnknown" : 0, "countIndependent" : 0, "countSelfCitation" : 0 }, { "type" : "Könyvrészlet", "typeEng" : "Chapter in Book", "code" : 25, "countUnknown" : 0, "countIndependent" : 0, "countSelfCitation" : 0 }, { "type" : "Könyv", "typeEng" : "Book", "code" : 23, "countUnknown" : 0, "countIndependent" : 0, "countSelfCitation" : 0 }, { "type" : "Egyéb konferenciaközlemény", "typeEng" : "Conference paper", "code" : 31, "countUnknown" : 0, "countIndependent" : 0, "countSelfCitation" : 0 }, { "type" : "Egyéb konferenciakötet", "typeEng" : "Conference proceedings", "code" : 32, "countUnknown" : 0, "countIndependent" : 0, "countSelfCitation" : 0 }, { "type" : "Oltalmi formák", "typeEng" : "Protection forms", "code" : 26, "countUnknown" : 0, "countIndependent" : 0, "countSelfCitation" : 0 }, { "type" : "Disszertáció", "typeEng" : "Thesis", "code" : 28, "countUnknown" : 0, "countIndependent" : 0, "countSelfCitation" : 0 }, { "type" : "Egyéb", "typeEng" : "Miscellaneous", "code" : 29, "countUnknown" : 0, "countIndependent" : 0, "countSelfCitation" : 0 }, { "type" : "Alkotás", "typeEng" : "Achievement", "code" : 22, "countUnknown" : 0, "countIndependent" : 0, "countSelfCitation" : 0 }, { "type" : "Kutatási adat", "typeEng" : "Research data", "code" : 33, "countUnknown" : 0, "countIndependent" : 0, "countSelfCitation" : 0 } ], "years" : [ { "year" : 2019, "publicationCount" : 0, "citationCount" : 2, "independentCitationCount" : 2, "citingPubCount" : 2, "independentCitingPubCount" : 2, "oaStats" : null, "oaStats2" : null }, { "year" : 2020, "publicationCount" : 0, "citationCount" : 1, "independentCitationCount" : 1, "citingPubCount" : 1, "independentCitingPubCount" : 1, "oaStats" : null, "oaStats2" : null }, { "year" : 2021, "publicationCount" : 0, "citationCount" : 1, "independentCitationCount" : 0, "citingPubCount" : 1, "independentCitingPubCount" : 0, "oaStats" : null, "oaStats2" : null }, { "year" : 2022, "publicationCount" : 0, "citationCount" : 7, "independentCitationCount" : 7, "citingPubCount" : 7, "independentCitingPubCount" : 7, "oaStats" : null, "oaStats2" : null }, { "year" : 2023, "publicationCount" : 0, "citationCount" : 2, "independentCitationCount" : 2, "citingPubCount" : 2, "independentCitingPubCount" : 2, "oaStats" : null, "oaStats2" : null }, { "year" : 2024, "publicationCount" : 0, "citationCount" : 1, "independentCitationCount" : 1, "citingPubCount" : 1, "independentCitingPubCount" : 1, "oaStats" : null, "oaStats2" : null } ] }, "ratings" : [ { "otype" : "SjrRating", "mtid" : 10738712, "link" : "/api/sjrrating/10738712", "label" : "sjr:Q1 (2018) Scopus - Analytical Chemistry JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 0731-7085 1873-264X", "listPos" : 28, "rankValue" : 0.24, "type" : "journal", "ratingType" : { "otype" : "RatingType", "mtid" : 10002, "link" : "/api/ratingtype/10002", "label" : "sjr", "code" : "sjr", "published" : true, "snippet" : true }, "subject" : { "otype" : "ClassificationExternal", "mtid" : 1602, "link" : "/api/classificationexternal/1602", "label" : "Scopus - Analytical Chemistry", "published" : true, "oldId" : 1602, "snippet" : true }, "ranking" : "Q1", "calculation" : "DIRECT", "published" : true, "snippet" : true } ], "ratingsForSort" : "Q1", "hasCitationDuplums" : false, "inSelectedPubs" : "10005745", "userChangeableUntil" : "2018-07-02T13:20:00.000+0000", "publishDate" : "2018-07-02T13:19:43.000+0000", "directInstitutesForSort" : "Alkalmazott Biotechnológia és Élelmiszertudomán... (BME / VBK); Richter Gedeon Nyrt.", "ownerAuthorCount" : 3, "ownerInstituteCount" : 11, "directInstituteCount" : 2, "authorCount" : 3, "contributorCount" : 0, "hasQualityFactor" : true, "link" : "/api/publication/3384042", "label" : "Kozma Bence et al. Comparison of multivariate data analysis techniques to improve glucose concentration prediction in mammalian cell cultivations by Raman spectroscopy. (2018) JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 0731-7085 1873-264X 158 269-279", "template" : "