https://m2.mtmt.hu/ hun 2026-09-20 22:40 JournalArticle 33834696 VALIDATED true 0 false 2026-05-22T23:03:37.684+0000 2023-06-29T07:42:18.806+0000 2023-05-19T10:38:14.849+0000 true 10035718 Sonnevend Kinga /api/admin/10035718 Admin true 10035718 2026-02-07T14:45:37.776+0000 2023-06-29T07:42:18.817+0000 true 10080205 Szalóky-Siki Ágnes /api/admin/10080205 Admin true Admin 10014568 /api/admin/10014568 Vándor Andrea true 10014568 true 2024-04-04T10:41:24.932+0000 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Kothalawala, W.J. Transcriptomic and Cellular Content Analysis of Colorectal Cancer by Combining Multiple Independent Cohorts true true /api/journal/10040271 REVIEWED true false 10040271 false 10040271 true 2155-384X Journal FOREIGN 14 2 e00517 e00517 9 2023 INTRODUCTION: By linking cellular content and molecular subtypes of colorectal cancer (CRC), we aim to uncover novel features useful for targeted therapy. Our first goal was to evaluate gene expression alterations linked to CRC pathogenesis, and then, we aimed to evaluate the cellular composition differences between normal colon mucosa and tumor and between different colon cancer molecular subtypes. METHODS: We collected microarray and RNA sequencing data of patients with CRC from the Genome Expression Omnibus and The Cancer Genome Atlas. We combined all cases and performed quantile normalization. Genes with a fold change of >2 were further investigated. We used xCell for cellular decomposition and CMScaller for molecular subtyping. For statistical analyses, the Kruskal-Wallis H test and Mann-Whitney U tests were performed with Bonferroni correction. RESULTS: We established an integrated database of normal colon and CRC using transcriptomic data of 1,082 samples. By using this data set, we identified genes showing the highest differential expression in colon tumors. The top genes were linked to calcium signaling, matrix metalloproteinases, and transcription factors. When compared with normal samples, CD4+ memory T cells, CD8+ naive T cells, CD8+ T cells, Th1 cells, Th2 cells, and regulatory T cells were enriched in tumor tissues. The ImmuneScore was decreased in tumor samples compared with normal samples. The CMS1 and CMS4 molecular subtypes were the most immunogenic, with the highest ImmuneScore but also high infiltration by CD8+ T cells, Th1 cells, and Th2 cells in CMS1 and B-cell subtypes and CD8+ T cells in CMS4. DISCUSSION: Our analysis uncovers features enabling advanced treatment selection and the development of novel therapies in CRC. Copyright © 2023 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of The American College of Gastroenterology. Funding 2028403 /api/funding/2028403 false true Funding 2028404 /api/funding/2028404 false true Funding 2028405 /api/funding/2028405 false true Funding 2028406 /api/funding/2028406 false true Funding 2028407 /api/funding/2028407 false true true 2023 true true true false true false GOLD 2026-05-23 true https://doi.org/10.14309/ctg.0000000000000517 6 0 6 5 1 6 6 6 6 6 6 5 5 5 1 0 6 5 6 5 0 7 7 Folyóiratcikk Journal Article 24 6 Könyvrészlet Chapter in Book 25 0 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2022 0 1 1 1 1 2023 0 2 1 2 1 2024 0 1 1 1 1 2025 0 2 2 2 2 Q1 false false false false 2023-06-28T07:42:18.615+0000 Belgyógyászati és Onkológiai Klinika (SE / AOK / K); Bioinformatika Tanszék (SE / AOK / I); II. Sz. Gyermekgyógyászati Klinika (SE / AOK / K); Onkológiai Biomarker Kutatócsoport (Lendület) (HRN TTK / MÉI) 2 26 4 2 0 true 10014568 Language 10002 /api/language/10002 Angol English true 2 true PersonAuthorship 107810790 /api/authorship/107810790 1 0.5 true false false Author 10077162 /api/author/10077162 Kothalawala William Jayasekara true true Kothalawala W.J. true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 107810791 /api/authorship/107810791 2 0.5 false true true Author 10000109 /api/author/10000109 Győrffy Balázs true 10000109 true Győrffy B. true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PublicationIdentifier 23585960 /api/publicationidentifier/23585960 PlainSource 6 /api/publicationsource/6 PublicationSourceType 10001 /api/publicationsourcetype/10001 true true true DOI DOI https://doi.org/@@@ true true 6 true GOLD true IDENTICAL 10.14309/ctg.0000000000000517 https://doi.org/10.14309/ctg.0000000000000517 false true PublicationIdentifier 24096125 /api/publicationidentifier/24096125 PlainSource 1 /api/publicationsource/1 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true IDENTICAL 000999434700007 https://www.webofscience.com/wos/woscc/full-record/000999434700007 true true PublicationIdentifier 23585959 /api/publicationidentifier/23585959 PlainSource 3 /api/publicationsource/3 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true Scopus Scopus http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@ true true 3 true IDENTICAL 85138005389 http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85138005389 false true PublicationIdentifier 24096225 /api/publicationidentifier/24096225 PlainSource 17 /api/publicationsource/17 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true PubMed PubMed http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=@@@&dopt=Abstract true true 17 true IDENTICAL 35858620 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=35858620&dopt=Abstract false true PublicationIdentifier 31376522 /api/publicationidentifier/31376522 PlainSource 40 /api/publicationsource/40 PublicationSourceType 10006 /api/publicationsourcetype/10006 true true true Egyéb URL Other URL @@@ true true 40 true NONE IDENTICAL https://www.scopus.com/inward/record.uri?eid=2-s2.0-85138005389&doi=10.14309%2Fctg.0000000000000517&partnerID=40&md5=64f80f9426022acadb1e0336b44f96a6 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85138005389&doi=10.14309%2Fctg.0000000000000517&partnerID=40&md5=64f80f9426022acadb1e0336b44f96a6 false true Classification 12215 /api/classification/12215 true true Keyword 65 /api/keyword/65 true 65 true Keyword 651 /api/keyword/651 true 651 true Keyword 3122 /api/keyword/3122 true 3122 true Keyword 4024 /api/keyword/4024 true 4024 true Keyword 4024 /api/keyword/4024 true 4024 true Keyword 8086 /api/keyword/8086 true 8086 true Keyword 8086 /api/keyword/8086 true 8086 true Keyword 19805 /api/keyword/19805 true 19805 true Keyword 19807 /api/keyword/19807 true 19807 true Keyword 1034819 /api/keyword/1034819 true 1034819 true Keyword 1034819 /api/keyword/1034819 true 1034819 true SjrRating 11380089 /api/sjrrating/11380089 20 0.24 journal RatingType 10002 /api/ratingtype/10002 sjr true true ClassificationExternal 2715 /api/classificationexternal/2715 true 2715 true Q1 DIRECT true true /api/publication/33834696 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10077162"><a href="/gui2/?type=authors&mode=browse&sel=10077162" target="_blank">Kothalawala W.J. (<span class="authorship-author-name">Kothalawala William Jayasekara</span> <span class="authorAux-mtmt"> orvostudomány</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Klinikum">K</span>/Belgyógyászati és Onkológiai Klinika</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10000109"><a href="/gui2/?type=authors&mode=browse&sel=10000109" target="_blank">Győrffy B. ✉ (<span class="authorship-author-name">Győrffy Balázs</span> <span class="authorAux-mtmt"> Onkológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Klinikum">K</span>/II. Sz. Gyermekgyógyászati Klinika; <span title="Természettudományi Kutatóközpont">TTK</span>/<span title="Molekuláris Élettudományi Intézet">MÉI</span>/Onkológiai Biomarker Kutatócsoport (Lendület); <span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Bioinformatika Tanszék</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;33834696" target="_blank">Transcriptomic and Cellular Content Analysis of Colorectal Cancer by Combining Multiple Independent Cohorts</a></div> <div> <span class="journal-title">CLINICAL AND TRANSLATIONAL GASTROENTEROLOGY</span> <span class="journal-issn">( <a target="_blank" href="https://portal.issn.org/resource/ISSN/2155-384X">2155-384X</a>)</span>: <span class="journal-volume">14</span> <span class="journal-issue">2</span> <span class="page"> Paper e00517. 9 p. </span> <span class="year">(2023)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="10.14309/ctg.0000000000000517" target="_blank" href="https://doi.org/10.14309/ctg.0000000000000517"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000999434700007" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000999434700007"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="85138005389" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85138005389"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="35858620" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=35858620&dopt=Abstract"> PubMed </a> </span> <span class="id identifier oa_NONE" title=" Nincs "> <a style="color:blue" title="https://www.scopus.com/inward/record.uri?eid=2-s2.0-85138005389&doi=10.14309%2Fctg.0000000000000517&partnerID=40&md5=64f80f9426022acadb1e0336b44f96a6" target="_blank" href="https://www.scopus.com/inward/record.uri?eid=2-s2.0-85138005389&doi=10.14309%2Fctg.0000000000000517&partnerID=40&md5=64f80f9426022acadb1e0336b44f96a6"> Egyéb URL </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Folyóirat szakterülete: Scopus - Gastroenterology&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 6</span> | Független: 5 | Függő: 1 | Nem jelölt: 0 | WoS jelölt: 6 | Scopus jelölt:&nbsp;6 | WoS/Scopus jelölt:&nbsp;6 | DOI jelölt:&nbsp;6 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;33834696&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 7 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 33834696</span> | <span class="status-data status-VALIDATED"> Egyeztetett </span> Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">Scopus</span> </div> <div class="funder"> (2018-2.1.17-TET-KR-00001) Támogató: NKFI, (2020-1.1.6-JOVO-2021-00013), (2018-1.3.1-VKE-2018-00032), Tématerületi Kiválósági Program(2020-4.1.1.-TKP2020), (the Bionic thematic programme of the Semmelweis University) </div> <div class="lastModified">Utolsó módosítás: 2023.06.29. 09:42 Szalóky-Siki Ágnes (SE_KK_Admin5_SZSA, admin) </div> <div class="lockedBy">Központi kezelésű 2024.04.04. 12:41 Vándor Andrea (VA, admin) </div> </div></div>