The recent human Monkeypox outbreak underlined the importance of studying basic biology
of orthopoxviruses. However, the transcriptome of its causative agent has not been
investigated before neither with short-, nor with long-read sequencing approaches.
This Oxford Nanopore long-read RNA-Sequencing dataset fills this gap. It will enable
the in-depth characterization of the transcriptomic architecture of the monkeypox
virus, and may even make possible to annotate novel host transcripts. Moreover, our
direct cDNA and native RNA sequencing reads will allow the estimation of gene expression
changes of both the virus and the host cells during the infection. Overall, our study
will lead to a deeper understanding of the alterations caused by the viral infection
on a transcriptome level.
