https://m2.mtmt.hu/ eng 2026-04-15 11:06 JournalArticle 33756254 APPROVED true 0 false 2026-03-04T08:30:18.014+0000 2025-02-26T12:07:35.335+0000 2023-04-18T08:24:20.161+0000 true 10080205 Szalóky-Siki Ágnes /api/admin/10080205 Admin true 2025-10-28T13:04:23.947+0000 2025-10-28T13:04:23.947+0000 Admin 10025544 /api/admin/10025544 Lévayné Deseő Katalin true 10025544 true 2024-04-17T12:20:09.648+0000 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Veres, J.M. CaMKIIα Promoter-Controlled Circuit Manipulations Target Both Pyramidal Cells and Inhibitory Interneurons in Cortical Networks true true /api/journal/10047435 REVIEWED true false 10047435 false 10047435 true 2373-2822 Journal FOREIGN 10 4 ENEURO.0070232023 ENEURO.0070232023 11 2023 A key assumption in studies of cortical functions is that excitatory principal neurons, but not inhibitory cells express calcium/calmodulin-dependent protein kinase II subunit α (CaMKIIα) resulting in a widespread use of CaMKIIα promoter-driven protein expression for principal cell manipulation and monitoring their activities. Using neuroanatomical and electrophysiological methods we demonstrate that in addition to pyramidal neurons, multiple types of cortical GABAegic cells are targeted by adeno-associated viral vectors (AAV) driven by the CaMKIIα promoter in both male and female mice. We tested the AAV5 and AAV9 serotype of viruses with either Channelrhodopsin 2 (ChR2)-mCherry or Archaerhodopsin-T-green fluorescent protein (GFP) constructs, with different dilutions. We show that in all cases, the reporter proteins can visualize a large fraction of different interneuron types, including parvalbumin (PV), somatostatin (SST), neuronal nitric oxide synthase (nNOS), neuropeptide Y (NPY), and cholecystokinin (CCK)-containing GABAergic cells, which altogether cover around 60% of the whole inhibitory cell population in cortical structures. Importantly, the expression of the excitatory opsin Channelrhodopsin 2 in the interneurons effectively drive spiking of infected GABAergic cells even if the immunodetectability of reporter proteins is ambiguous. Thus, our results challenge the use of CaMKIIα promoter-driven protein expression as a selective tool in targeting cortical glutamatergic neurons using viral vectors. Funding 2028784 /api/funding/2028784 false true Funding 2039148 /api/funding/2039148 false true true true 2023 true true true false true false GOLD 2026-03-04 true https://doi.org/10.1523/ENEURO.0070-23.2023 50 0 50 50 0 50 50 49 45 50 50 50 50 50 0 0 50 50 50 50 0 6 6 Folyóiratcikk Journal Article 24 50 Könyvrészlet Chapter in Book 25 0 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2023 0 3 3 3 3 2024 0 21 21 21 21 2025 0 25 25 25 25 2026 0 1 1 1 1 Q1 false 10010484 false false false 2023-04-17T08:29:54.780+0000 Doktori Iskola (SE); Hálózat-Idegélettani Kutatócsoport (HRN KOKI); Kísérleti Orvostudományi Kutatóintézet 4 15 3 4 0 true 10025544 Language 10002 /api/language/10002 Angol English true 2 true PersonAuthorship 107366974 /api/authorship/107366974 1 0.25 true false false Author 10018814 /api/author/10018814 Veres Judit true 10018814 true Veres J.M. true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 107366975 /api/authorship/107366975 2 0.25 true false false Author 10039582 /api/author/10039582 Andrási Tibor true 10039582 true Andrasi T. true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 107366976 /api/authorship/107366976 3 0.25 false false false Author 10065453 /api/author/10065453 Nagy-Pál Petra true true Nagy-Pal P. true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 107366977 /api/authorship/107366977 4 0.25 false true true Author 10010484 /api/author/10010484 Hájos Norbert true 10010484 true Hajos N. true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PublicationIdentifier 23387163 /api/publicationidentifier/23387163 PlainSource 6 /api/publicationsource/6 PublicationSourceType 10001 /api/publicationsourcetype/10001 true true true DOI DOI https://doi.org/@@@ true true 6 true GOLD true IDENTICAL 10.1523/ENEURO.0070-23.2023 https://doi.org/10.1523/ENEURO.0070-23.2023 false true PublicationIdentifier 23388609 /api/publicationidentifier/23388609 SwordSource 36 /api/publicationsource/36 PublicationSourceType 10007 /api/publicationsourcetype/10007 true true true REAL REAL http://real.mtak.hu/@@@ true true 36 true GOLD true IDENTICAL 163920 http://real.mtak.hu/163920 false true PublicationIdentifier 23583717 /api/publicationidentifier/23583717 PlainSource 1 /api/publicationsource/1 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true IDENTICAL 000970481500002 https://www.webofscience.com/wos/woscc/full-record/000970481500002 false true PublicationIdentifier 23387162 /api/publicationidentifier/23387162 PlainSource 3 /api/publicationsource/3 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true Scopus Scopus http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@ true true 3 true IDENTICAL 85152173334 http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85152173334 false true PublicationIdentifier 23387197 /api/publicationidentifier/23387197 PlainSource 17 /api/publicationsource/17 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true PubMed PubMed http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=@@@&dopt=Abstract true true 17 true IDENTICAL 36963833 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=36963833&dopt=Abstract false true Keyword 1204765 /api/keyword/1204765 true 1204765 true Keyword 1210559 /api/keyword/1210559 true 1210559 true Keyword 1289742 /api/keyword/1289742 true 1289742 true Keyword 1399375 /api/keyword/1399375 true 1399375 true Keyword 1519062 /api/keyword/1519062 true true SjrRating 11397364 /api/sjrrating/11397364 365 0.24 journal RatingType 10002 /api/ratingtype/10002 sjr true true ClassificationExternal 2701 /api/classificationexternal/2701 true 2701 true Q1 DIRECT true true /api/publication/33756254 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10018814"><a href="/gui2/?type=authors&mode=browse&sel=10018814" target="_blank">Veres J.M. (<span class="authorship-author-name">Veres Judit</span> <span class="authorAux-mtmt"> Idegélettan</span>) </a> </span> <span class="author-affil"><span title="Institute of Experimental Medicine">IEM</span>/Network Neurophysiology; Institute of Experimental Medicine</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10039582"><a href="/gui2/?type=authors&mode=browse&sel=10039582" target="_blank">Andrasi T.<sup>*</sup> (<span class="authorship-author-name">Andrási Tibor</span> <span class="authorAux-mtmt"> Elektrofiziológia</span>) </a> </span> <span class="author-affil"><span title="Institute of Experimental Medicine">IEM</span>/Network Neurophysiology; Institute of Experimental Medicine</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10065453"><a href="/gui2/?type=authors&mode=browse&sel=10065453" target="_blank">Nagy-Pal P. (<span class="authorship-author-name">Nagy-Pál Petra</span> <span class="authorAux-mtmt"> biológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis University">SU</span>/School of PhD Studies; <span title="Institute of Experimental Medicine">IEM</span>/Network Neurophysiology; Institute of Experimental Medicine</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10010484"><a href="/gui2/?type=authors&mode=browse&sel=10010484" target="_blank">Hajos N. ✉ (<span class="authorship-author-name">Hájos Norbert</span> <span class="authorAux-mtmt"> Neurobiológia</span>) </a> </span> <span class="author-affil"><span title="Institute of Experimental Medicine">IEM</span>/Network Neurophysiology; Institute of Experimental Medicine</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;33756254" target="_blank">CaMKIIα Promoter-Controlled Circuit Manipulations Target Both Pyramidal Cells and Inhibitory Interneurons in Cortical Networks</a></div> <div> <span class="journal-title">ENEURO</span> <span class="journal-issn">( <a target="_blank" href="https://portal.issn.org/resource/ISSN/2373-2822">2373-2822</a>)</span>: <span class="journal-volume">10</span> <span class="journal-issue">4</span> <span class="page"> Paper ENEURO.0070232023. 11 p. </span> <span class="year">(2023)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Language: English | </span> <span class="identifiers"> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="10.1523/ENEURO.0070-23.2023" target="_blank" href="https://doi.org/10.1523/ENEURO.0070-23.2023"> DOI </a> </span> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="163920" target="_blank" href="http://real.mtak.hu/163920"> REAL </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000970481500002" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000970481500002"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="85152173334" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85152173334"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="36963833" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=36963833&dopt=Abstract"> PubMed </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Journal subject: Scopus - Medicine (miscellaneous)&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q1</div> <div class="journal-subject">Journal subject: Scopus - Neuroscience (miscellaneous)&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q2</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="" class="citingPub-count">Citing papers: 50</span> | Independent citation: 50 | Self citation: 0 | Unknown citation: 0 | Number of citations in WoS: 49 | Number of citations in Scopus:&nbsp;45 | WoS/Scopus assigned:&nbsp;50 | Number of citations with DOI:&nbsp;50 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;33756254&sort=publishedYear,desc&sort=title"> Number of cited publications: 6 </a> </div> <div class="mtid"><span class="long-pub-mtid">Publication: 33756254</span> | <span class="status-data status-APPROVED"> Published </span> Core Citing | <span class="type-subtype">Journal Article ( Article ) </span> | <span class="pub-category">Scientific</span> | <span class="publication-sourceOfData">Scopus</span> </div> <div class="funder"> (K131893) Funder: NKFI, Hungarian National Brain Research Program(2017-1.2.1-NKP-2017-00002) </div> <div class="notDuplums"><a target="_blank" href="/api/publication/33756254/notduplums">1 marked as not duplum</a> </div> <div class="lastModified">Last Modified: 2025.02.26. 13:07 Gusztáv Ladányi (MTMT API user, admin) </div> <div class="lockedBy">Centrally managed 2024.04.17. 14:20 Katalin Lévayné Deseő (MTMT Központi admin) </div> </div></div>