https://m2.mtmt.hu/ hun 2026-09-21 13:28 JournalArticle 33684822 ADMIN_APPROVED true Department of Biochemistry, Institute of Biochemistry and Molecular Biology, Semmelweis University, Budapest, 1094, Hungary Department of Chemistry, Rutgers University, Newark, NJ 07102, United States Cited By :5 Export Date: 15 January 2025 Correspondence Address: Ambrus, A.; Department of Biochemistry, Hungary; email: ambrus.attila@med.semmelweis-univ.hu Correspondence Address: Jordan, F.; Department of Chemistry, United States; email: frjordan@newark.rutgers.edu 0 false 2026-05-22T19:27:55.129+0000 2026-04-20T09:38:11.625+0000 2023-03-06T19:42:59.462+0000 true 10014144 Ozohanics Olivér /api/author/10014144 Author true 10014144 2026-04-21T08:27:23.865+0000 2024-05-28T07:29:46.665+0000 true 10045364 Fi Tamás /api/admin/10045364 Admin true 10045364 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Ozohanics, Oliver Probing the E1o-E2o and E1a-E2o Interactions in Binary Subcomplexes of the Human 2-Oxoglutarate Dehydrogenase and 2-Oxoadipate Dehydrogenase Complexes by Chemical Cross-Linking Mass Spectrometry and Molecular Dynamics Simulation true true /api/journal/10003252 REVIEWED true false 10003252 false 10003252 true 1661-6596 1422-0067 Journal FOREIGN 24 5 4555 4555 21 2023 The human 2-oxoglutarate dehydrogenase complex (hOGDHc) is a key enzyme in the tricarboxylic acid cycle and is one of the main regulators of mitochondrial metabolism through NADH and reactive oxygen species levels. Evidence was obtained for formation of a hybrid complex between the hOGDHc and its homologue the 2-oxoadipate dehydrogenase complex (hOADHc) in the L-lysine metabolic pathway, suggesting a crosstalk between the two distinct pathways. Findings raised fundamental questions about the assembly of hE1a (2-oxoadipate-dependent E1 component) and hE1o (2-oxoglutarate-dependent E1) to the common hE2o core component. Here we report chemical cross-linking mass spectrometry (CL-MS) and molecular dynamics (MD) simulation analyses to understand assembly in binary subcomplexes. The CL-MS studies revealed the most prominent loci for hE1o-hE2o and hE1a-hE2o interactions and suggested different binding modes. The MD simulation studies led to the following conclusions: (i) The N-terminal regions in E1s are shielded by, but do not interact directly with hE2o. (ii) The hE2o linker region exhibits the highest number of H-bonds with the N-terminus and α/β1 helix of hE1o, yet with the interdomain linker and α/β1 helix of hE1a. (iii) The C-termini are involved in dynamic interactions in complexes, suggesting the presence of at least two conformations in solution. Funding 2024931 /api/funding/2024931 false true Funding 2025112 /api/funding/2025112 false true Funding 2025113 /api/funding/2025113 false true Funding 2025114 /api/funding/2025114 false true true 2023 true true true false true false GOLD 2026-05-22 true https://doi.org/10.3390/ijms24054555 7 0 7 6 1 7 7 6 7 7 7 6 6 6 1 0 7 6 7 6 0 5 5 Folyóiratcikk Journal Article 24 7 Könyvrészlet Chapter in Book 25 0 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2023 0 3 3 3 3 2024 0 2 1 2 1 2025 0 2 2 2 2 D1 false 10000177 false false false 2023-03-26T11:32:29.792+0000 Biokémiai Tanszék (SE / AOK / I / BMBI); Biokémiai és Molekuláris Biológiai Intézet (SE / AOK / I) 2 22 2 5 0 true Language 10002 /api/language/10002 Angol English true 2 true PersonAuthorship 106936567 /api/authorship/106936567 1 0.2 true false false Author 10014144 /api/author/10014144 Ozohanics Olivér true 10014144 true Ozohanics Oliver true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 106936568 /api/authorship/106936568 2 0.2 false false false Zhang Xu true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 106936569 /api/authorship/106936569 3 0.2 false false false Nemeria Natalia S. true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 106936570 /api/authorship/106936570 4 0.2 false true true Author 10000177 /api/author/10000177 Ambrus Attila true 10000177 true Ambrus Attila true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 106936571 /api/authorship/106936571 5 0.2 false true true Jordan Frank true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PublicationIdentifier 23197542 /api/publicationidentifier/23197542 PlainSource 6 /api/publicationsource/6 PublicationSourceType 10001 /api/publicationsourcetype/10001 true true true DOI DOI https://doi.org/@@@ true true 6 true GOLD true IDENTICAL 10.3390/ijms24054555 https://doi.org/10.3390/ijms24054555 false true PublicationIdentifier 23312336 /api/publicationidentifier/23312336 PlainSource 1 /api/publicationsource/1 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true IDENTICAL 000947318900001 https://www.webofscience.com/wos/woscc/full-record/000947318900001 true true PublicationIdentifier 23285860 /api/publicationidentifier/23285860 PlainSource 3 /api/publicationsource/3 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true Scopus Scopus http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@ true true 3 true IDENTICAL 85149809926 http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85149809926 false true PublicationIdentifier 23285861 /api/publicationidentifier/23285861 PlainSource 17 /api/publicationsource/17 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true PubMed PubMed http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=@@@&dopt=Abstract true true 17 true IDENTICAL 36901986 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=36901986&dopt=Abstract false true PublicationIdentifier 31302440 /api/publicationidentifier/31302440 PlainSource 40 /api/publicationsource/40 PublicationSourceType 10006 /api/publicationsourcetype/10006 true true true Egyéb URL Other URL @@@ true true 40 true NONE IDENTICAL https://www.scopus.com/inward/record.uri?eid=2-s2.0-85149809926&doi=10.3390%2Fijms24054555&partnerID=40&md5=ecba134d84f81ffeafce526584f136b2 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85149809926&doi=10.3390%2Fijms24054555&partnerID=40&md5=ecba134d84f81ffeafce526584f136b2 false true PublicationIdentifier 31860400 /api/publicationidentifier/31860400 PlainSource 40 /api/publicationsource/40 PublicationSourceType 10006 /api/publicationsourcetype/10006 true true true Egyéb URL Other URL @@@ true true 40 true NONE IDENTICAL https://www.scopus.com/pages/publications/85149809926?origin=resultslist https://www.scopus.com/pages/publications/85149809926?origin=resultslist false true SjrRating 11388415 /api/sjrrating/11388415 7 0.1 journal RatingType 10002 /api/ratingtype/10002 sjr true true ClassificationExternal 1604 /api/classificationexternal/1604 true 1604 true D1 DIRECT true true /api/publication/33684822 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10014144"><a href="/gui2/?type=authors&mode=browse&sel=10014144" target="_blank">Ozohanics Oliver (<span class="authorship-author-name">Ozohanics Olivér</span> <span class="authorAux-mtmt"> Tömegspektrometria</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Biokémiai és Molekuláris Biológiai Intézet; <span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/<span title="Biokémiai és Molekuláris Biológiai Intézet">BMBI</span>/Biokémiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Zhang Xu </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Nemeria Natalia S. </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10000177"><a href="/gui2/?type=authors&mode=browse&sel=10000177" target="_blank">Ambrus Attila<sup>**</sup> ✉ (<span class="authorship-author-name">Ambrus Attila</span> <span class="authorAux-mtmt"> Biokémia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Biokémiai és Molekuláris Biológiai Intézet; <span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/<span title="Biokémiai és Molekuláris Biológiai Intézet">BMBI</span>/Biokémiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Jordan Frank ✉ </span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;33684822" target="_blank">Probing the E1o-E2o and E1a-E2o Interactions in Binary Subcomplexes of the Human 2-Oxoglutarate Dehydrogenase and 2-Oxoadipate Dehydrogenase Complexes by Chemical Cross-Linking Mass Spectrometry and Molecular Dynamics Simulation</a></div> <div> <span class="journal-title">INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/1661-6596">1661-6596</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1422-0067">1422-0067</a>)</span>: <span class="journal-volume">24</span> <span class="journal-issue">5</span> <span class="page"> Paper 4555. 21 p. </span> <span class="year">(2023)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="10.3390/ijms24054555" target="_blank" href="https://doi.org/10.3390/ijms24054555"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000947318900001" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000947318900001"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="85149809926" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85149809926"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="36901986" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=36901986&dopt=Abstract"> PubMed </a> </span> <span class="id identifier oa_NONE" title=" Nincs "> <a style="color:blue" title="https://www.scopus.com/inward/record.uri?eid=2-s2.0-85149809926&doi=10.3390%2Fijms24054555&partnerID=40&md5=ecba134d84f81ffeafce526584f136b2" target="_blank" href="https://www.scopus.com/inward/record.uri?eid=2-s2.0-85149809926&doi=10.3390%2Fijms24054555&partnerID=40&md5=ecba134d84f81ffeafce526584f136b2"> Egyéb URL </a> </span> <span class="id identifier oa_NONE" title=" Nincs "> <a style="color:blue" title="https://www.scopus.com/pages/publications/85149809926?origin=resultslist" target="_blank" href="https://www.scopus.com/pages/publications/85149809926?origin=resultslist"> Egyéb URL </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Folyóirat szakterülete: Scopus - Inorganic Chemistry&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Organic Chemistry&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Spectroscopy&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Computer Science Applications&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Medicine (miscellaneous)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Physical and Theoretical Chemistry&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Catalysis&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q2</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Molecular Biology&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q2</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 7</span> | Független: 6 | Függő: 1 | Nem jelölt: 0 | WoS jelölt: 6 | Scopus jelölt:&nbsp;7 | WoS/Scopus jelölt:&nbsp;7 | DOI jelölt:&nbsp;7 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;33684822&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 5 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 33684822</span> | <span class="status-data status-ADMIN_APPROVED"> Admin láttamozott </span> Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">Bibtex</span> </div> <div class="funder"> (TKP2021-EGA-25), Hungarian National Brain Research Program(2017-1.2.1-NKP-2017-00002), (STIA-OTKA-2021), OTKA(143627) </div> <div class="lastModified">Utolsó módosítás: 2026.04.20. 11:38 Kajtár Virág Anna (SE_AOK_Biokemia_Admin5_KV, admin) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Megjegyzés</u>: Department of Biochemistry, Institute of Biochemistry and Molecular Biology, Semmelweis University, Budapest, 1094, Hungary Department of Chemistry, Rutgers University, Newark, NJ 07102, United States Cited By :5 Export Date: 15 January 2025 Correspondence Address: Ambrus, A.; Department of Biochemis...</pre> </div></div>