https://m2.mtmt.hu/ hun 2026-09-20 21:09 JournalArticle 33667258 APPROVED true HansaBioMed Life Sciences Ltd., Tallinn, Estonia School of Natural Sciences and Health, Tallinn University, Tallinn, Estonia Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary Eötvös Loránd Research Network, Translational Extracellular Vesicle Research Group, Semmelweis University, Budapest, Hungary Eötvös Loránd Research Network, Institute of Experimental Medicine, Budapest, Hungary Hungarian Center of Excellence Molecular Medicine, Extracellular Vesicle Research Group, Semmelweis University, Budapest, Hungary Exosomics SpA, Siena, Italy Cited By :1 Export Date: 13 February 2024 CODEN: JTHOA Correspondence Address: Zarovni, N.; HansaBioMed Life Sciences Ltd., Mäealuse 2/1, Estonia; email: nzarovni@exosomics.eu Chemicals/CAS: Biomarkers; Lipopolysaccharides; Lipoproteins Funding details: 2014-2020.4.02.21-0398 Funding details: Horizon 2020 Framework Programme, H2020, 739593, TKP-2021-Terapias-EGA-23 Funding details: Hungarian Scientific Research Fund, OTKA, NVKP_16-1-2016- 0017, VEKOP-2.3.2-16-2016-00002, VEKOP-2.3.3-15-2017-00016 Funding details: Horizon 2020, H2020-MSCA-ITN-2017-722148, NVKP_16-1-2016-0017 Funding text 1: This work was supported by following funding programs: European Union’s Horizon 2020 research and innovation program under the grant agreement number H2020-MSCA-ITN-2017-722148 (TRAIN EV); European Regional Development Fund Enterprise Estonia’s Applied Research Program under the grant agreement number 2014-2020.4.02.21-0398 (EVREM), National Scientific Research Program of Hungary (OTKA) NVKP_16-1-2016- 0017, Higher Education Institutional Excellence Program – Therapeutic development, Ministry for National Economy of Hungary under the grant number VEKOP-2.3.2-16-2016-00002, VEKOP-2.3.3-15-2017-00016, European Union’s Horizon 2020 research and innovation program under the grant agreement number 739593, and the grant TKP-2021-Terapias-EGA-23. Funding text 2: This work was supported by following funding programs: European Union's Horizon 2020 research and innovation program under the grant agreement number H2020-MSCA-ITN-2017-722148 (TRAIN EV); European Regional Development Fund Enterprise Estonia's Applied Research Program under the grant agreement number 2014-2020.4.02.21-0398 (EVREM), National Scientific Research Program of Hungary (OTKA) NVKP_16-1-2016- 0017, Higher Education Institutional Excellence Program – Therapeutic development, Ministry for National Economy of Hungary under the grant number VEKOP-2.3.2-16-2016-00002, VEKOP-2.3.3-15-2017-00016, European Union's Horizon 2020 research and innovation program under the grant agreement number 739593, and the grant TKP-2021-Terapias-EGA-23.This work was supported by following funding programmes: European Union's Horizon 2020 research and innovation programme under the grant agreement number H2020-MSCA-ITN-2017-722148 (TRAIN EV); European Regional Development Fund Enterprise Estonia's Applied Research Program under the grant agreement number 2014-2020.4.02.21-0398 (EVREM), National Scientific Research Program of Hungary (OTKA) NVKP_16-1-2016-0017, Higher Education Institutional Excellence Program – Therapeutic development, Ministry for National Economy of Hungary under the grant number VEKOP-2.3.2-16-2016-00002, VEKOP-2.3.3-15-2017-00016, European Union's Horizon 2020 research and innovation program under the grant agreement number 739593, and the grant TKP-2021-Terapias-EGA-23. D.M. and D.K. contributed equally to this work. Conceptualization, D.M. and N.Z.; Methodology; D.M. and D.K.; Data Curation, D.M. D.K. and A.K.; Formal Analysis, D.M. D.K. and A.K.; Validation, D.M. D.K. E.I.B. and N.Z.; Writing—Original Draft Preparation, D.M. and D.K.; Writing—Review and Editing N.Z. E.I.B. A.K. and K.K.; Supervision, N.Z. E.I.B. and K.K. All authors read and approved the final manuscript. There are no competing interests to disclose. Funding information Funding text 3: This work was supported by following funding programmes: European Union’s Horizon 2020 research and innovation programme under the grant agreement number H2020-MSCA-ITN-2017-722148 (TRAIN EV); European Regional Development Fund Enterprise Estonia’s Applied Research Program under the grant agreement number 2014-2020.4.02.21-0398 (EVREM), National Scientific Research Program of Hungary (OTKA) NVKP_16-1-2016-0017, Higher Education Institutional Excellence Program – Therapeutic development, Ministry for National Economy of Hungary under the grant number VEKOP-2.3.2-16-2016-00002, VEKOP-2.3.3-15-2017-00016, European Union’s Horizon 2020 research and innovation program under the grant agreement number 739593, and the grant TKP-2021-Terapias-EGA-23. 0 false 2026-08-06T09:55:09.849+0000 2025-02-26T12:07:00.148+0000 2023-02-27T14:42:12.059+0000 true 10019965 Ádám Adrienn /api/admin/10019965 Admin true 10019965 2026-05-21T09:12:33.733+0000 2026-05-21T09:12:33.733+0000 Admin 10067876 /api/admin/10067876 Ladányi Gusztáv true true Admin 10014568 /api/admin/10014568 Vándor Andrea true 10014568 true 2024-10-17T16:44:08.551+0000 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Mladenović, Danilo Acidification of blood plasma facilitates the separation and analysis of extracellular vesicles true true /api/journal/10001276 REVIEWED true false 10001276 false 10001276 true 1538-7933 1538-7836 Journal FOREIGN 21 4 1032 1042 1032 11 2023 Background: Blood plasma is available with minimal invasive sampling, it has significant diagnostic utility, and it is a valuable source of extracellular vesicles (EVs). Neverthe- less, rich protein content, the presence of lipoproteins (LPs) that share similar biophysical properties, and relatively low abundance of EVs, especially those of rare subpopulations, make any downstream application a very challenging task. The growing evidence of the intricate surface interactome of EVs, and the association of EVs with LPs, impose further challenges during EV purification, detection, and biomarker analyses. Objectives: In this study, we tackled the fundamental issues of plasma EV yield and LP co-isolation and their implications in the subsequent marker analyses. Methods: Moderate acidification of plasma was combined with size exclusion chromatography (SEC) and/or differential centrifugation (DC) to disrupt LPs and improve recovery of EVs and their subsequent detection by immunoassays and single-particle analysis methods. Results: Our results demonstrate a surprisingly efficient enrichment of EVs (up to 3.3-fold higher than at pH 7) and partial depletion of LPs (up to 61.2%). Acidification of blood plasma samples enabled a quick single-step isoelectric precipitation of up to 20.4% of EVs directly from plasma, upon short low-speed centrifugation. Conclusion: Thus, acidification holds potential as a simple and inexpensive methodological step, which improves the efficacy of plasma EV enrichment and may have implications in future biomarker discoveries. 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/api/reference/38806521 42 false true /api/publication/33667258 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" >Mladenović Danilo </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10071730"><a href="/gui2/?type=authors&mode=browse&sel=10071730" target="_blank">Khamari Delaram (<span class="authorship-author-name">Khamari Delaram</span> <span class="authorAux-mtmt"> Extracellular vesicles</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Genetikai, Sejt- és Immunbiológiai Intézet; <span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/<span title="Genetikai, Sejt- és Immunbiológiai Intézet">GSII</span>/ELKH-SE Transzlációs Extracelluláris Vezikula Kutatócsoport</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10009113"><a href="/gui2/?type=authors&mode=browse&sel=10009113" target="_blank">Kittel Ágnes (<span class="authorship-author-name">Kittel Ágnes</span> <span class="authorAux-mtmt"> Sejtbiológia</span>) </a> </span> <span class="author-affil">Kísérleti Orvostudományi Kutatóintézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Koort Kairi </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10015596"><a href="/gui2/?type=authors&mode=browse&sel=10015596" target="_blank">Buzás Edit I. (<span class="authorship-author-name">Buzás Edit Irén</span> <span class="authorAux-mtmt"> Immunbiológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Genetikai, Sejt- és Immunbiológiai Intézet; <span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/<span title="Genetikai, Sejt- és Immunbiológiai Intézet">GSII</span>/ELKH-SE Transzlációs Extracelluláris Vezikula Kutatócsoport; <span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/<span title="Genetikai, Sejt- és Immunbiológiai Intézet">GSII</span>/HCEMM-SE Extracelluláris Vezikula Kutatócsoport</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Zarovni Nataša ✉ </span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;33667258" target="_blank">Acidification of blood plasma facilitates the separation and analysis of extracellular vesicles</a></div> <div> <span class="journal-title">JOURNAL OF THROMBOSIS AND HAEMOSTASIS</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/1538-7933">1538-7933</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1538-7836">1538-7836</a>)</span>: <span class="journal-volume">21</span> <span class="journal-issue">4</span> <span class="page"> pp 1032-1042 </span> <span class="year">(2023)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="10.1016/j.jtha.2023.01.007" target="_blank" href="https://doi.org/10.1016/j.jtha.2023.01.007"> DOI </a> </span> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="163293" target="_blank" href="https://real.mtak.hu/163293"> REAL </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="001054154700001" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/001054154700001"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="85150989212" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85150989212"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="36774282" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=36774282&dopt=Abstract"> PubMed </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Folyóirat szakterülete: Scopus - Medicine (miscellaneous)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Hematology&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 11</span> | Független: 9 | Függő: 2 | Nem jelölt: 0 | WoS jelölt: 11 | Scopus jelölt:&nbsp;10 | WoS/Scopus jelölt:&nbsp;11 | DOI jelölt:&nbsp;11 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;33667258&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 7 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 33667258</span> | <span class="status-data status-APPROVED"> Nyilvános </span> Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">kézi felvitel</span> </div> <div class="funder"> (VEKOP-2.3.2-16-2016-00002) Támogató: NKFIH, (VEKOP-2.3.3.-15-2017-00016), HCEMM(739593) Támogató: Horizon 2020, (TKP-2021-Terapias-EGA-23), National Heart Program(NVKP-16-1-2016-0017) Támogató: NKFIH </div> <div class="lastModified">Utolsó módosítás: 2025.02.26. 13:07 Ladányi Gusztáv (MTMT API user, admin) </div> <div class="lockedBy">Központi kezelésű 2024.10.17. 18:44 Ladányi Gusztáv (MTMT API user, admin) Vándor Andrea (VA, admin) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Megjegyzés</u>: HansaBioMed Life Sciences Ltd., Tallinn, Estonia School of Natural Sciences and Health, Tallinn University, Tallinn, Estonia Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary Eötvös Loránd Research Network, Translational Extracellular Vesicle Research Group, Semmelweis University, Bud...</pre> </div></div>