Gyógyszerészet, farmakogenomika, gyógyszerkutatás és tervezés, gyógyszeres kezelés
Extracellular vesicles (EVs) are cell-derived, membrane-enclosed particles with the
potential for a wide range of future therapeutic applications. However, EVs have almost
always been administered by direct injection, likely hindering their efficacy because
of rapid clearance from the injection site. The present study aimed to incorporate
medium-sized extracellular vesicles (mEVs) into fast-dissolving electrospun polyvinylpyrrolidone-based
nanofibers to explore the storage-dependent structure–activity relationship of the
resulting nanofibrous formulations. Aqueous polyvinylpyrrolidone-based precursor solutions
were selected for the electrospinning process. The presence of EVs in the electrospun
samples was confirmed by transmission electron microscopy, flow cytometry, and confocal
laser scanning microscope. The results indicate that the fibrous structure of the
samples was preserved until the end of the 12-week storage period. Furthermore, regardless
of the storage temperature (4 °C or room temperature), nanofibers and nanofiber-associated
EVs were present throughout the experimental period. Incorporating EVs into a stable
solid polymeric delivery base could preserve their stability; meanwhile, according
to the characteristics of the polymer, their targeted and controlled release can be
achieved.
