https://m2.mtmt.hu/ hun 2026-09-21 22:46 JournalArticle 33263015 VALIDATED true Funding Agency and Grant Number: Hungarian Scientific Research Fund (OTKA) [K128188, K134968, K142322, PD134858]; National Research, Development and Innovation Office [2018-1.1.1-MKI-2018-00081]; Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences [BO/764/20]; Higher Education Institutional Excellence Program of Semmelweis University [VEKOP-2.1.7-17-2016-00383, EFOP-3.6.3-VEKOP-16-2017-00009] Funding text: We thank Gyorgy Varadi and the FACS core facility for their valuable help. We thank Ildiko Szcsne Pulinka, Judit Szcs, Vivien Karl, Ferencne Zajer, and Diana Szeregnyei for their excellent laboratory assistance. We thank Vanessza Laura Vegi and Dora Bokor for proofreading the manuscript and Zsuzsa Bartos and Zsofia Rakvacs for their valuable help. The project was supported by grants K128188, K134968, and K142322 to E.W. and PD134858 to P.I.K. from the Hungarian Scientific Research Fund (OTKA) and grant 2018-1.1.1-MKI-2018-00081 to E.W. from the National Research, Development and Innovation Office. P.I.K. is a recipient of the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences (BO/764/20). S.L.K. was supported by grants VEKOP-2.1.7-17-2016-00383 and EFOP-3.6.3-VEKOP-16-2017-00009 from the Higher Education Institutional Excellence Program of Semmelweis University. 0 false 2026-07-31T07:41:54.699+0000 2025-08-19T19:39:29.263+0000 2022-11-23T10:42:30.053+0000 true 10015754 Várady György /api/author/10015754 Author Várady György (Membránbiológia) true 10015754 2025-08-12T21:03:08.396+0000 2025-02-17T09:37:28.009+0000 true 10097128 Sass Dávid /api/admin/10097128 Admin Sass Dávid (SZTE admin5) true true false true 24 24 /api/publicationtype/24 PublicationType Folyóiratcikk 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType Folyóiratcikk 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType Szakcikk (Folyóiratcikk) 101 true 10000059 true 1 /api/category/1 Category Tudományos true 1 Kulcsár, P.I. true true /api/journal/10015678 REVIEWED NATURE COMMUNICATIONS 2041-1723 true false 10015678 false 10015678 true 2041-1723 Journal FOREIGN 13 1 6858 6858 11 2022 Several advancements have been made to SpCas9, the most widely used CRISPR/Cas genome editing tool, to reduce its unwanted off-target effects. The most promising approach is the development of increased-fidelity nuclease (IFN) variants of SpCas9, however, their fidelity has increased at the cost of reduced activity. SuperFi-Cas9 has been developed recently, and it has been described as a next-generation high-fidelity SpCas9 variant, free from the drawbacks of first-generation IFNs. In this study, we characterize the on-target activity and the off-target propensity of SuperFi-Cas9 in mammalian cells, comparing it to first-generation IFNs. SuperFi-Cas9 demonstrates strongly reduced activity but high fidelity features that are in many aspects similar to those of some first-generation variants, such as evo- and HeFSpCas9. SuperFi-cytosine (CBE3) and -adenine (ABE7.10) base editors, as well as SuperFi-prime editor show no meaningful activity. When combined with ABE8e, SuperFi-Cas9, similarly to HeFSpCas9, executes DNA editing with high activity as well as high specificity reducing both bystander and SpCas9-dependent off-target base editing. © 2022, The Author(s). 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[Ligeti, Zoltán Mihály (Biológia), szerző] Biokémiai Intézet (HRN SZBK); Multidiszciplináris Orvostudományok Doktori Iskola (SZTE / DI) 3 0.2 false false false Author 10069721 /api/author/10069721 Ligeti Zoltán Mihály (Biológia) Ligeti Zoltán Mihály true true Ligeti Z. true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 104423210 /api/authorship/104423210 Krausz, S.L. 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Regionális Tudományok Bizottsága:A nemzetközi NATURE COMMUNICATIONS 2041-1723 RatingType 10010 /api/ratingtype/10010 IX. Regionális Tudományok Bizottsága RegTB Institute 14100 /api/institute/14100 Regionális Tudományok Bizottsága IXGJO RTB [1901-] 0 false true false Regionális Tudományok Bizottsága Committee on Regional Studies true 14100 true true true A nemzetközi false true SjrRating 11282711 /api/sjrrating/11282711 sjr:D1 (2022) Scopus - Biochemistry, Genetics and Molecular Biology (miscellaneous) NATURE COMMUNICATIONS 2041-1723 2041-1723 10 0.1 journal RatingType 10002 /api/ratingtype/10002 sjr sjr true true ClassificationExternal 1301 /api/classificationexternal/1301 Scopus - Biochemistry, Genetics and Molecular Biology (miscellaneous) true 1301 true D1 DIRECT true true Reference 35989668 /api/reference/35989668 1. Anzalone, A.V., Koblan, L.W., Liu, D.R., Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors (2020) Nat. 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(2022) NATURE COMMUNICATIONS 2041-1723 13 1<div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10038471"><a href="/gui2/?type=authors&mode=browse&sel=10038471" target="_blank">Kulcsár P.I. (<span class="authorship-author-name">Kulcsár Péter István</span> <span class="authorAux-mtmt"> Molekuláris biológia</span>) </a> </span> <span class="author-affil"><span title="Természettudományi Kutatóközpont">TTK</span>/Enzimológiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10055848"><a href="/gui2/?type=authors&mode=browse&sel=10055848" target="_blank">Tálas A.^* (<span class="authorship-author-name">Tálas András</span> <span class="authorAux-mtmt"> Molekuláris Biológus</span>) </a> </span> <span class="author-affil"><span title="Természettudományi Kutatóközpont">TTK</span>/Enzimológiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10069721"><a href="/gui2/?type=authors&mode=browse&sel=10069721" target="_blank">Ligeti Z. (<span class="authorship-author-name">Ligeti Zoltán Mihály</span> <span class="authorAux-mtmt"> Biológia</span>) </a> </span> <span class="author-affil"><span title="Szegedi Biológiai Kutatóközpont">SZBK</span>/Biokémiai Intézet; <span title="Szegedi Tudományegyetem">SZTE</span>/<span title="Doktori Iskolák">DI</span>/Multidiszciplináris Orvostudományok Doktori Iskola</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10061281"><a href="/gui2/?type=authors&mode=browse&sel=10061281" target="_blank">Krausz S.L. (<span class="authorship-author-name">Krausz Sarah</span> <span class="authorAux-mtmt"> géntechnológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/Doktori Iskola</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10001345"><a href="/gui2/?type=authors&mode=browse&sel=10001345" target="_blank">Welker E. ✉ (<span class="authorship-author-name">Welker Ervin</span> <span class="authorAux-mtmt"> Fehérje feltekeredés</span>) </a> </span> <span class="author-affil"><span title="Szegedi Biológiai Kutatóközpont">SZBK</span>/Biokémiai Intézet; <span title="Természettudományi Kutatóközpont">TTK</span>/Enzimológiai Intézet</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;33263015" target="_blank">SuperFi-Cas9 exhibits remarkable fidelity but severely reduced activity yet works effectively with ABE8e</a></div> <div> <span class="journal-title">NATURE COMMUNICATIONS</span> <span class="journal-issn">( <a target="_blank" href="https://portal.issn.org/resource/ISSN/2041-1723">2041-1723</a>)</span>: <span class="journal-volume">13</span> <span class="journal-issue">1</span> <span class="page"> Paper 6858. 11 p. </span> <span class="year">(2022)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="10.1038/s41467-022-34527-8" target="_blank" href="https://doi.org/10.1038/s41467-022-34527-8"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000882306500004" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000882306500004"> WoS </a> </span> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:black" title="159226" target="_blank" href="https://real.mtak.hu/159226"> REAL </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="85141654679" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85141654679"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="36369279" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=36369279&dopt=Abstract"> PubMed </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Folyóirat szakterülete: Scopus - Biochemistry, Genetics and Molecular Biology (miscellaneous)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Chemistry (miscellaneous)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Physics and Astronomy (miscellaneous)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> <div class="journal-subject">Regionális Tudományok Bizottsága IXGJO RTB [1901-]&nbsp;&nbsp;&nbsp;A nemzetközi</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 36</span> | Független: 35 | Függő: 1 | Nem jelölt: 0 | WoS jelölt: 34 | Scopus jelölt:&nbsp;36 | WoS/Scopus jelölt:&nbsp;36 | DOI jelölt:&nbsp;36 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;33263015&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 4 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 33263015</span> | <span class="status-data status-VALIDATED"> Egyeztetett </span> Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">Scopus</span> </div> <div class="funder"> (K128188), (K134968), (K142322), (PD134858) Támogató: OTKA, (2018-1.1.1-MKI-2018-00081) Támogató: NKFIH, (BO/764/20) Támogató: Bolyai János Kutatási Ösztöndíj, (VEKOP-2.1.7-17-2016-00383), Az orvos-, egészségtudományi- és gyógyszerészképzés tudományos műhelyeinek fejlesztése(EFOP-3.6.3-VEKOP-16-2017-00009) Támogató: EFOP-VEKOP </div> <div class="lastModified">Utolsó módosítás: 2025.08.19. 21:39 Bernátskyné Babus Csilla (SZTE admin4) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Megjegyzés</u>: Funding Agency and Grant Number: Hungarian Scientific Research Fund (OTKA) [K128188, K134968, K142322, PD134858]; National Research, Development and Innovation Office [2018-1.1.1-MKI-2018-00081]; Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences [BO/764/20]; Higher Education Institutional Excellence Program of Semmelweis University [VEKOP-2.1.7-17-2016-00383, EFOP-3.6.3-VEK...</pre> </div></div>