The objective of this study was to optimize the extrusion processing for production
of maximum peptides spectra in multigrain flakes. The results showed that maximum
peptides spectra were produced at extrusion condition of 160 ??C temperature and 100
rpm screw speed and minimum peptides spectra were observed at 120 ??C temperature
and 300 rpm. Among proximal composition, highest changes were observed in fat content
and thus, significantly affected the atherogenicity and thrombogenicity indices of
products. The products with higher peptides spectra (16) showed higher 2,2-diphenyl-1-picrylhydrazyl
(DPPH) (48.63%), ferric reducing antioxidant power (FRAP) (48.63 ?? 4.81 mmol Fe+2/g
protein) and superoxide radical-scavenging activity (SRSA) (39.05 ?? 4.51%). Similarly,
higher peptides spectra product showed higher angiotensin converting enzyme (ACE)
(86.01 ?? 5.22%) inhibition and inhibition of protein denaturation (IP) (38.12 ??
2.89%). Thus optimization of extrusion process for bioactive peptides can be used
for production of biological active products and healthily production system.