https://m2.mtmt.hu/ hun 2026-09-21 19:42 JournalArticle 33114978 VALIDATED true 0 false 2026-05-22T09:02:02.507+0000 2025-08-04T12:11:42.008+0000 2022-09-27T16:48:33.075+0000 true 10023793 Csépányi-Kömi Roland /api/author/10023793 Author true 10023793 2025-08-04T12:11:35.749+0000 2025-08-04T12:11:35.749+0000 2025-08-04T12:11:42.241+0000 true 10062062 Gere Tamásné /api/admin/10062062 Admin true 10062062 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Wisniewski, Éva A Novel BRET-Based GAP assay reveals phosphorylation-dependent regulation of the RAC-specific GTPase activating protein ARHGAP25 true true /api/journal/1569 REVIEWED true false 1569 false 1569 true 0892-6638 1530-6860 Journal FOREIGN 36 11 e22584 e22584 19 2022 ARHGAP25, a RAC‐specific GTPase activating protein (GAP), is an essential regulator of phagocyte effector functions such as phagocytosis, superoxide production, and transendothelial migration. Furthermore, its complex role in tumor behavior has recently been recognized. We previously demonstrated that phosphorylation of serine 363 in ARHGAP25 regulates hematopoietic stem cells and progenitor cells in mouse bone marrow. However, the significance of other potential phosphorylation sites of ARHGAP25 remained unknown. Now, we developed a novel, real‐time bioluminescence resonance energy transfer (BRET) assay to monitor the GAP activity of ARHGAP25 in vitro. Using this approach, we revealed that phosphorylation of S363 and S488, but not that of S379‐380, controls ARHGAP25's RACGAP activity. On the other hand, we found in granulocyte‐differentiated human PLB‐985 cells that superoxide production and actin depolymerization are regulated by residues S363 and S379‐380. The present data demonstrate the value of our BRET‐GAP assay and show that different phosphorylation patterns regulate ARHGAP25's GAP activity and its effect on superoxide production and phagocytosis. Funding 2019299 /api/funding/2019299 false true Funding 2019300 /api/funding/2019300 false true Funding 2019301 /api/funding/2019301 false true Funding 2019302 /api/funding/2019302 false true true true 2022 true true true false true false GOLD 2026-05-22 true https://doi.org/10.1096/fj.202200689R 4 0 4 3 1 4 4 4 4 4 4 3 3 3 1 0 4 3 4 3 0 10 10 Folyóiratcikk Journal Article 24 4 Könyvrészlet Chapter in Book 25 0 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 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class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10052421"><a href="/gui2/?type=authors&mode=browse&sel=10052421" target="_blank">Wisniewski Éva (<span class="authorship-author-name">Wisniewski Éva</span> <span class="authorAux-mtmt"> Sejtélettan</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Élettani Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10085536"><a href="/gui2/?type=authors&mode=browse&sel=10085536" target="_blank">Czárán Domonkos<sup>*</sup> (<span class="authorship-author-name">Czárán Domonkos Tamás</span> <span class="authorAux-mtmt"> Élettan</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Élettani Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10098338"><a href="/gui2/?type=authors&mode=browse&sel=10098338" target="_blank">Kovács Fanni (<span class="authorship-author-name">Kovács Fanni</span> <span class="authorAux-mtmt"> csecsemő- és gyermekgyógyászat</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Élettani Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Bahurek Enikő </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10080217"><a href="/gui2/?type=authors&mode=browse&sel=10080217" target="_blank">Németh Afrodité (<span class="authorship-author-name">Németh Afrodité</span> <span class="authorAux-mtmt"> biológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Élettani Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10085543"><a href="/gui2/?type=authors&mode=browse&sel=10085543" target="_blank">Sasvári Péter (<span class="authorship-author-name">Sasvári Péter</span> <span class="authorAux-mtmt"> Élettan</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Élettani Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10015491"><a href="/gui2/?type=authors&mode=browse&sel=10015491" target="_blank">Szanda Gergő (<span class="authorship-author-name">Szanda Gergő</span> <span class="authorAux-mtmt"> Molekuláris Élettan</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Élettani Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10025208"><a href="/gui2/?type=authors&mode=browse&sel=10025208" target="_blank">Pettkó-Szandtner Aladár (<span class="authorship-author-name">Pettkó-Szandtner Aladár</span> <span class="authorAux-mtmt"> növénybiológia</span>) </a> </span> <span class="author-affil"><span title="Szegedi Biológiai Kutatóközpont">SZBK</span>/Biokémiai Intézet; <span title="Szegedi Biológiai Kutatóközpont">SZBK</span>/Központi Laboratóriumok</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10025070"><a href="/gui2/?type=authors&mode=browse&sel=10025070" target="_blank">Klement Éva (<span class="authorship-author-name">Klement Éva</span> <span class="authorAux-mtmt"> biokémia</span>) </a> </span> <span class="author-affil"><span title="Szegedi Biológiai Kutatóközpont">SZBK</span>/Biokémiai Intézet; <span title="Szegedi Biológiai Kutatóközpont">SZBK</span>/Központi Laboratóriumok</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="426140"><a href="/gui2/?type=authors&mode=browse&sel=426140" target="_blank">Ligeti Erzsébet (<span class="authorship-author-name">Ligeti Erzsébet</span> <span class="authorAux-mtmt"> Sejtélettan</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Élettani Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10023793"><a href="/gui2/?type=authors&mode=browse&sel=10023793" target="_blank">Csépányi-Kömi Roland ✉ (<span class="authorship-author-name">Csépányi-Kömi Roland</span> <span class="authorAux-mtmt"> élettan</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Élettani Intézet</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;33114978" target="_blank">A Novel BRET-Based GAP assay reveals phosphorylation-dependent regulation of the RAC-specific GTPase activating protein ARHGAP25</a></div> <div> <span class="journal-title">FASEB JOURNAL</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/0892-6638">0892-6638</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1530-6860">1530-6860</a>)</span>: <span class="journal-volume">36</span> <span class="journal-issue">11</span> <span class="page"> Paper e22584. 19 p. </span> <span class="year">(2022)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="10.1096/fj.202200689R" target="_blank" href="https://doi.org/10.1096/fj.202200689R"> DOI </a> </span> <span 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class="journal-subject">Folyóirat szakterülete: Scopus - Biotechnology&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Genetics&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Medicine (miscellaneous)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Molecular Biology&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q2</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 4</span> | Független: 3 | Függő: 1 | Nem jelölt: 0 | WoS jelölt: 4 | Scopus jelölt:&nbsp;4 | WoS/Scopus jelölt:&nbsp;4 | DOI jelölt:&nbsp;4 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;33114978&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 10 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 33114978</span> | <span class="status-data status-VALIDATED"> Egyeztetett </span> Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">kézi felvitel</span> </div> <div class="funder"> (GINOP-2.3.2-15-2016-00032), HCEMM(739593) Támogató: Horizon 2020, (János Bolyai Research Scholarship), (ÚNKP-20-5-SE-20) Támogató: ÚNKP </div> <div class="lastModified">Utolsó módosítás: 2025.08.04. 14:11 Gere Tamásné (SE_KK_Admin5_GT, admin) </div> </div></div>