https://m2.mtmt.hu/ hun 2026-10-24 16:11 JournalArticle 33111578 VALIDATED true Beáta Szeitz and Zsolt Megyesfalvi contributed equally. Balázs Döme, Karin Schelch, and Melinda Rezeli share the last authorship 0 false 2026-10-22T18:52:52.730+0000 2026-05-27T08:51:02.549+0000 2022-09-26T12:48:08.935+0000 true 10081340 Pirmann Tamás /api/admin/10081340 Admin true 2026-04-09T13:19:41.963+0000 2026-04-09T13:19:41.963+0000 2026-01-06T08:30:55.599+0000 true 10003134 Wolf György /api/admin/10003134 Admin true 10003134 Admin 10073918 /api/admin/10073918 Kovács Marcell true true 2023-10-04T07:54:51.165+0000 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Szeitz, Beáta In-depth proteomic analysis reveals unique subtype-specific signatures in human small-cell lung cancer true true /api/journal/10044311 REVIEWED true false 10044311 false 10044311 true 2001-1326 2001-1326 Journal FOREIGN 12 9 e1060 e1060 26 2022 Small-cell lung cancer (SCLC) molecular subtypes have been primarily characterized based on the expression pattern of the following key transcription regulators: ASCL1 (SCLC-A), NEUROD1 (SCLC-N), POU2F3 (SCLC-P) and YAP1 (SCLC-Y). Here, we investigated the proteomic landscape of these molecular subsets with the aim to identify novel subtype-specific proteins of diagnostic and therapeutic relevance.Pellets and cell media of 26 human SCLC cell lines were subjected to label-free shotgun proteomics for large-scale protein identification and quantitation, followed by in-depth bioinformatic analyses. Proteomic data were correlated with the cell lines' phenotypic characteristics and with public transcriptomic data of SCLC cell lines and tissues.Our quantitative proteomic data highlighted that four molecular subtypes are clearly distinguishable at the protein level. The cell lines exhibited diverse neuroendocrine and epithelial-mesenchymal characteristics that varied by subtype. A total of 367 proteins were identified in the cell pellet and 34 in the culture media that showed significant up- or downregulation in one subtype, including known druggable proteins and potential blood-based markers. Pathway enrichment analysis and parallel investigation of transcriptomics from SCLC cell lines outlined unique signatures for each subtype, such as upregulated oxidative phosphorylation in SCLC-A, DNA replication in SCLC-N, neurotrophin signalling in SCLC-P and epithelial-mesenchymal transition in SCLC-Y. Importantly, we identified the YAP1-driven subtype as the most distinct SCLC subgroup. Using sparse partial least squares discriminant analysis, we identified proteins that clearly distinguish four SCLC subtypes based on their expression pattern, including potential diagnostic markers for SCLC-Y (e.g. GPX8, PKD2 and UFO).We report for the first time, the protein expression differences among SCLC subtypes. By shedding light on potential subtype-specific therapeutic vulnerabilities and diagnostic biomarkers, our results may contribute to a better understanding of SCLC biology and the development of novel therapies. 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Patológiai és Kísérleti Rákkutató Intézet (SE / AOK / I); Mellkassebészeti Klinika (SE / AOK / K); Országos Korányi Pulmonológiai Intézet; Országos Onkológiai Intézet; Patológiai és Kísérleti Rákkutató Intézet (SE / AOK / I) 9 25 7 21 0 true 10073918 Language 10002 /api/language/10002 Angol English true 2 true PersonAuthorship 103522229 /api/authorship/103522229 1 0.048 true false false Author 10074938 /api/author/10074938 Szeitz Beáta true true Szeitz Beáta true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 103522230 /api/authorship/103522230 2 0.048 true false false Author 10071898 /api/author/10071898 Megyesfalvi Zsolt true true Megyesfalvi Zsolt true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 103522231 /api/authorship/103522231 3 0.048 false false false Woldmar Nicole true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 103522232 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/api/keyword/1246870 true 1246870 true Keyword 1495974 /api/keyword/1495974 true true Keyword 1495995 /api/keyword/1495995 true true Keyword 1586775 /api/keyword/1586775 true true SjrRating 11309163 /api/sjrrating/11309163 175 0.1 journal RatingType 10002 /api/ratingtype/10002 sjr true true ClassificationExternal 2701 /api/classificationexternal/2701 true 2701 true D1 DIRECT true true /api/publication/33111578 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10074938"><a href="/gui2/?type=authors&mode=browse&sel=10074938" target="_blank">Szeitz Beáta (<span class="authorship-author-name">Szeitz Beáta</span> <span class="authorAux-mtmt"> Patológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Klinikum">K</span>/Belgyógyászati és Onkológiai Klinika</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10071898"><a href="/gui2/?type=authors&mode=browse&sel=10071898" target="_blank">Megyesfalvi Zsolt<sup>*</sup> (<span class="authorship-author-name">Megyesfalvi Zsolt</span> <span class="authorAux-mtmt"> Orvostudomány</span>) </a> </span> <span class="author-affil">Országos Onkológiai Intézet; <span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Klinikum">K</span>/Mellkassebészeti Klinika</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Woldmar Nicole </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Valkó Zsuzsanna </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Schwendenwein Anna </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10080554"><a href="/gui2/?type=authors&mode=browse&sel=10080554" target="_blank">Bárány Nándor (<span class="authorship-author-name">Bárány Nándor</span> <span class="authorAux-mtmt"> Tüdőrák</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/I. Sz. Patológiai és Kísérleti Rákkutató Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10000740"><a href="/gui2/?type=authors&mode=browse&sel=10000740" target="_blank">Paku Sándor (<span class="authorship-author-name">Paku Sándor</span> <span class="authorAux-mtmt"> Onkológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Patológiai és Kísérleti Rákkutató Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10037177"><a href="/gui2/?type=authors&mode=browse&sel=10037177" target="_blank">László Viktória (<span class="authorship-author-name">László Viktória</span> <span class="authorAux-mtmt"> tumorbiologia</span>) </a> </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Kiss Helga </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10028428"><a href="/gui2/?type=authors&mode=browse&sel=10028428" target="_blank">Bugyik Edina (<span class="authorship-author-name">Bugyik Edina</span> <span class="authorAux-mtmt"> Biológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/I. Sz. Patológiai és Kísérleti Rákkutató Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Lang Christian </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10023803"><a href="/gui2/?type=authors&mode=browse&sel=10023803" target="_blank">Szász Attila Marcell (<span class="authorship-author-name">Szász Attila Marcell</span> <span class="authorAux-mtmt"> emlőpatologia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Bioinformatika Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Pizzatti Luciana </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10003497"><a href="/gui2/?type=authors&mode=browse&sel=10003497" target="_blank">Bogos Krisztina (<span class="authorship-author-name">Bogos Krisztina</span> <span class="authorAux-mtmt"> Tüdőgyógyászat, Klinikai Onkológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Klinikum">K</span>/Mellkassebészeti Klinika</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Hoda Mir Alireza </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Hoetzenecker Konrad </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Marko-Varga György </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Horvatovich Peter </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10000046"><a href="/gui2/?type=authors&mode=browse&sel=10000046" target="_blank">Döme Balázs<sup>**</sup> ✉ (<span class="authorship-author-name">Döme Balázs</span> <span class="authorAux-mtmt"> Tumor indukált angiogenezis</span>) </a> </span> <span class="author-affil">Országos Onkológiai Intézet; <span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Klinikum">K</span>/Mellkassebészeti Klinika; Országos Korányi Pulmonológiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Schelch Karin<sup>**</sup> </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Rezeli Melinda ✉ </span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;33111578" target="_blank">In-depth proteomic analysis reveals unique subtype-specific signatures in human small-cell lung cancer</a></div> <div> <span class="journal-title">CLINICAL AND TRANSLATIONAL MEDICINE</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/2001-1326">2001-1326</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/2001-1326">2001-1326</a>)</span>: <span class="journal-volume">12</span> <span class="journal-issue">9</span> <span class="page"> Paper e1060. 26 p. </span> <span class="year">(2022)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="10.1002/ctm2.1060" target="_blank" href="https://doi.org/10.1002/ctm2.1060"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000859631300001" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000859631300001"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="85154582161" target="_blank" href="https://www.scopus.com/search/submit/citedby.uri?origin=resultslist&src=s&eid=2-s2.0-85154582161"> Scopus-Cit </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:black" title="85154582161" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85154582161"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="36149789" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=36149789&dopt=Abstract"> PubMed </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Folyóirat szakterülete: Scopus - Medicine (miscellaneous)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Molecular Medicine&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 27</span> | Független: 14 | Függő: 13 | Nem jelölt: 0 | WoS jelölt: 24 | Scopus jelölt:&nbsp;22 | WoS/Scopus jelölt:&nbsp;26 | DOI jelölt:&nbsp;25 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;33111578&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 5 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 33111578</span> | <span class="status-data status-VALIDATED"> Egyeztetett </span> Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">kézi felvitel</span> </div> <div class="funder"> Az orvos-, egészségtudományi- és gyógyszerészképzés tudományos műhelyeinek fejlesztése(EFOP-3.6.3-VEKOP-16-2017-00009) Támogató: EFOP-VEKOP, (KH130356), (KKP126790), (KNN121510), (2020-1.1.6-JOVO), (TKP2021-EGA-33), (ÚNKP-20-3), (UNKP-21-3), (ÚNKP-19-4) </div> <div class="lastModified">Utolsó módosítás: 2026.05.27. 10:51 Kozma Bernadett (KK_AOK_Mellkassebeszet_Admin5_KB, admin) </div> <div class="lockedBy">Központi kezelésű 2023.10.04. 09:54 Kovács Marcell (MTMT Közp., admin) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Megjegyzés</u>: Beáta Szeitz and Zsolt Megyesfalvi contributed equally. Balázs Döme, Karin Schelch, and Melinda Rezeli share the last authorship</pre> </div></div>