https://m2.mtmt.hu/ hun 2026-09-23 09:49 JournalArticle 33092478 VALIDATED true 0 false 2026-09-04T06:32:04.618+0000 2026-03-26T09:45:52.137+0000 2022-09-14T09:43:46.066+0000 true 10071451 Kulin Anna /api/author/10071451 Author true 2026-03-25T17:15:34.784+0000 2026-03-25T17:15:34.784+0000 2023-10-17T13:57:04.125+0000 true 10083379 Belházyné Szombathelyi Ágnes /api/admin/10083379 Admin true true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Szabó, Edit Selective Fluorescent Probes for High-Throughput Functional Diagnostics of the Human Multidrug Transporter P-Glycoprotein (ABCB1) true true /api/journal/10003252 REVIEWED true false 10003252 false 10003252 true 1661-6596 1422-0067 Journal FOREIGN 23 18 10599 10599 12 2022 The multidrug transporter ABCB1 (MDR1, Pgp) plays an important role in the absorption, distribution, metabolism, and elimination of a wide range of pharmaceutical compounds. Functional investigation of the ABCB1 expression is also essential in many diseases, including drug-resistant cancer, inflammatory conditions, or Alzheimer disease. In this study, we examined the potential interaction of the ABCB1 multidrug transporter with a group of commercially available viability dyes that are generally considered not to penetrate into intact cells. Here, we demonstrate that the slow cellular accumulation of TO-PRO™-1 (TP1) or TO-PRO™-3 (TP3) was strongly inhibited by ABCB1-dependent dye extrusion. TP1/3 dye accumulation was not affected by the presence of ABCC1 or ABCG2, while this uptake was increased to the level in the ABCB1-negative cells by a specific P-glycoprotein inhibitor, Tariquidar. We suggest that TP compounds can be used as highly sensitive, selective, non-toxic, and stable dyes to examine the functional expression and properties of the ABCB1 multidrug transporter, especially in microplate-based high-throughput flow cytometry assays. In addition, we demonstrate the applicability of the TP dyes to efficiently select and separate even a very low number of Pgp-expressing intact cells. Funding 2017744 /api/funding/2017744 false true true 2022 true true true false true false GOLD 2026-09-04 true https://doi.org/10.3390/ijms231810599 2 0 2 2 0 2 2 2 2 2 2 2 2 2 0 0 2 2 2 2 0 11 11 Folyóiratcikk Journal Article 24 2 Könyvrészlet Chapter in Book 25 0 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2025 0 2 2 2 2 D1 false 10015754,10040526,10071451 false false false 2022-09-13T10:46:59.861+0000 Biofizikai és Sugárbiológiai Intézet (SE / AOK / I); Doktori Iskola (SE); Enzimológiai Intézet (TTK) 6 50 3 6 0 true Language 10002 /api/language/10002 Angol English true 2 true PersonAuthorship 103392703 /api/authorship/103392703 1 0.167 true false false Author 10040526 /api/author/10040526 Szabó Edit Zsuzsanna true 10040526 true Szabó Edit true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 103392704 /api/authorship/103392704 2 0.167 false false false Author 10071451 /api/author/10071451 Kulin Anna true true Kulin Anna true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 103392705 /api/authorship/103392705 3 0.167 false false false Author 10068186 /api/author/10068186 Jezsó Bálint true true Jezsó Bálint true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 103392706 /api/authorship/103392706 4 0.167 false false false Author 10049521 /api/author/10049521 Kucsma Nóra true 10049521 true Kucsma Nóra true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 103392707 /api/authorship/103392707 5 0.167 false false true Author 1241938 /api/author/1241938 Sarkadi Balázs true 1241938 true Sarkadi Balázs true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 103392708 /api/authorship/103392708 6 0.167 false true false Author 10015754 /api/author/10015754 Várady György true 10015754 true Várady György true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PublicationIdentifier 21677893 /api/publicationidentifier/21677893 PlainSource 6 /api/publicationsource/6 PublicationSourceType 10001 /api/publicationsourcetype/10001 true true true DOI DOI https://doi.org/@@@ true true 6 true GOLD true IDENTICAL 10.3390/ijms231810599 https://doi.org/10.3390/ijms231810599 false true PublicationIdentifier 21776448 /api/publicationidentifier/21776448 PlainSource 1 /api/publicationsource/1 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true IDENTICAL 000856416400001 https://www.webofscience.com/wos/woscc/full-record/000856416400001 true true PublicationIdentifier 21776446 /api/publicationidentifier/21776446 PlainSource 3 /api/publicationsource/3 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true Scopus Scopus http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@ true true 3 true IDENTICAL 85138389052 http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85138389052 true true PublicationIdentifier 21776452 /api/publicationidentifier/21776452 PlainSource 17 /api/publicationsource/17 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true PubMed PubMed http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=@@@&dopt=Abstract true true 17 true IDENTICAL 36142507 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=36142507&dopt=Abstract true true SjrRating 11289558 /api/sjrrating/11289558 7 0.1 journal RatingType 10002 /api/ratingtype/10002 sjr true true ClassificationExternal 1604 /api/classificationexternal/1604 true 1604 true D1 DIRECT true true /api/publication/33092478 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10040526"><a href="/gui2/?type=authors&mode=browse&sel=10040526" target="_blank">Szabó Edit (<span class="authorship-author-name">Szabó Edit Zsuzsanna</span> <span class="authorAux-mtmt"> biológus</span>) </a> </span> <span class="author-affil"><span title="Természettudományi Kutatóközpont">TTK</span>/Enzimológiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10071451"><a href="/gui2/?type=authors&mode=browse&sel=10071451" target="_blank">Kulin Anna (<span class="authorship-author-name">Kulin Anna</span> <span class="authorAux-mtmt"> biológus</span>) </a> </span> <span class="author-affil"><span title="Természettudományi Kutatóközpont">TTK</span>/Enzimológiai Intézet; <span title="Semmelweis Egyetem">SE</span>/Doktori Iskola</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10068186"><a href="/gui2/?type=authors&mode=browse&sel=10068186" target="_blank">Jezsó Bálint (<span class="authorship-author-name">Jezsó Bálint</span> <span class="authorAux-mtmt"> Sejtbiológia</span>) </a> </span> <span class="author-affil"><span title="Természettudományi Kutatóközpont">TTK</span>/Enzimológiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10049521"><a href="/gui2/?type=authors&mode=browse&sel=10049521" target="_blank">Kucsma Nóra (<span class="authorship-author-name">Kucsma Nóra</span> <span class="authorAux-mtmt"> Membránbiológia</span>) </a> </span> <span class="author-affil"><span title="Természettudományi Kutatóközpont">TTK</span>/Enzimológiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="1241938"><a href="/gui2/?type=authors&mode=browse&sel=1241938" target="_blank">Sarkadi Balázs ✉ (<span class="authorship-author-name">Sarkadi Balázs</span> <span class="authorAux-mtmt"> biofizika</span>) </a> </span> <span class="author-affil"><span title="Természettudományi Kutatóközpont">TTK</span>/Enzimológiai Intézet; <span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Biofizikai és Sugárbiológiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10015754"><a href="/gui2/?type=authors&mode=browse&sel=10015754" target="_blank">Várady György (<span class="authorship-author-name">Várady György</span> <span class="authorAux-mtmt"> Membránbiológia</span>) </a> </span> <span class="author-affil"><span title="Természettudományi Kutatóközpont">TTK</span>/Enzimológiai Intézet</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;33092478" target="_blank">Selective Fluorescent Probes for High-Throughput Functional Diagnostics of the Human Multidrug Transporter P-Glycoprotein (ABCB1)</a></div> <div> <span class="journal-title">INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/1661-6596">1661-6596</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1422-0067">1422-0067</a>)</span>: <span class="journal-volume">23</span> <span class="journal-issue">18</span> <span class="page"> Paper 10599. 12 p. </span> <span class="year">(2022)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="10.3390/ijms231810599" target="_blank" href="https://doi.org/10.3390/ijms231810599"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000856416400001" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000856416400001"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="85138389052" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85138389052"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="36142507" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=36142507&dopt=Abstract"> PubMed </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Folyóirat szakterülete: Scopus - Inorganic Chemistry&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Spectroscopy&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Computer Science Applications&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Medicine (miscellaneous)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Organic Chemistry&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Physical and Theoretical Chemistry&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Catalysis&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q2</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Molecular Biology&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q2</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 2</span> | Független: 2 | Függő: 0 | Nem jelölt: 0 | WoS jelölt: 2 | Scopus jelölt:&nbsp;2 | WoS/Scopus jelölt:&nbsp;2 | DOI jelölt:&nbsp;2 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;33092478&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 11 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 33092478</span> | <span class="status-data status-VALIDATED"> Egyeztetett </span> Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">kézi felvitel</span> </div> <div class="funder"> (NKFIH K-128011) </div> <div class="lastModified">Utolsó módosítás: 2026.03.26. 10:45 Pécsi Éva (MTMT Közp 2,3, admin) </div> </div></div>