Comprehensive Collection and Prediction of ABC Transmembrane Protein Structures in the AI Era of Structural Biology

Tordai, Hedvig [Tordai, Hedvig (molekuláris biológia), szerző] Biofizikai és Sugárbiológiai Intézet (SE / AOK / I); Suhajda, Erzsebet* [Suhajda, Erzébet (Fizika), szerző] Részben rendezett rendszerek csoport (Wigner FK / SZFI / KFO); Sillitoe, Ian; Nair, Sreenath; Varadi, Mihaly; Hegedus, Tamas ✉ [Hegedűs, Tamás (bioinformatika, b...), szerző] Biofizikai és Sugárbiológiai Intézet (SE / AOK / I)

Angol nyelvű Szakcikk (Folyóiratcikk) Tudományos
Megjelent: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 1661-6596 1422-0067 23 (16) Paper: 8877 , 16 p. 2022
  • SJR Scopus - Spectroscopy: D1
Azonosítók
Támogatások:
  • (K127961) Támogató: NKFIH
  • (K137610)
  • (2020-2.1.1-ED-2021-00179)
  • (HEGEDU20I0)
Szakterületek:
  • Fizika
The number of unique transmembrane (TM) protein structures doubled in the last four years, which can be attributed to the revolution of cryo-electron microscopy. In addition, AlphaFold2 (AF2) also provided a large number of predicted structures with high quality. However, if a specific protein family is the subject of a study, collecting the structures of the family members is highly challenging in spite of existing general and protein domain-specific databases. Here, we demonstrate this and assess the applicability and usability of automatic collection and presentation of protein structures via the ABC protein superfamily. Our pipeline identifies and classifies transmembrane ABC protein structures using the PFAM search and also aims to determine their conformational states based on special geometric measures, conftors. Since the AlphaFold database contains structure predictions only for single polypeptide chains, we performed AF2-Multimer predictions for human ABC half transporters functioning as dimers. Our AF2 predictions warn of possibly ambiguous interpretation of some biochemical data regarding interaction partners and call for further experiments and experimental structure determination. We made our predicted ABC protein structures available through a web application, and we joined the 3D-Beacons Network to reach the broader scientific community through platforms such as PDBe-KB.
Hivatkozás stílusok: IEEEACMAPAChicagoHarvardCSLMásolásNyomtatás
2022-12-06 17:26