https://m2.mtmt.hu/ hun 2026-05-01 09:21 JournalArticle 33062017 APPROVED true Department of Pharmacology and Pharmacotherapy, Semmelweis University, Nagyvárad tér 4., Budapest, 1089, Hungary Institute of Experimental Medicine, Eötvös Lóránd Research Network, Szigony utca 43., Budapest, 1083, Hungary Cited By :2 Export Date: 29 August 2024 CODEN: NERED Correspondence Address: Vizi, E.S.; Department of Pharmacology and Pharmacotherapy, Nagyvárad tér 4., Hungary; email: esvizi@koki.hu Chemicals/CAS: calcium ion, 14127-61-8; cocaine, 50-36-2, 53-21-4, 5937-29-1; nisoxetine, 53179-07-0, 57754-86-6; noradrenalin, 1407-84-7, 51-41-2; phenylephrine, 532-38-7, 59-42-7, 61-76-7; prazosin, 19216-56-9, 19237-84-4; Adrenergic alpha-Agonists; Adrenergic alpha-Antagonists; Norepinephrine; Phenylephrine; Prazosin; Receptors, Adrenergic, alpha-1 Funding details: Semmelweis Egyetem, TKP2021-EGA-25 Funding details: Emberi Eroforrások Minisztériuma, EMMI Funding text 1: Open access funding provided by Semmelweis University. This work was supported by Higher Education Institutional Excellence Programme of the Ministry of Human Capacities in Hungary, within the framework of the Neurology Thematic Programme of Semmelweis University (TKP 2021 EGA-25). E. Sylvester Vizi has received research support from the Eötvös Lóránd Research Network. 0 false 2026-04-29T09:10:54.428+0000 2025-02-25T23:12:54.487+0000 2022-08-23T11:30:29.078+0000 true 10080205 Szalóky-Siki Ágnes /api/admin/10080205 Admin true 2026-01-12T09:30:46.617+0000 2026-01-12T09:30:46.617+0000 Admin 10066925 /api/admin/10066925 Nemec Évi true true Admin 10067876 /api/admin/10067876 Ladányi Gusztáv true true 2024-10-17T16:20:19.023+0000 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Al-Khrasani, M. The Pharmacological Effects of Phenylephrine are Indirect, Mediated by Noradrenaline Release from the Cytoplasm true true /api/journal/3344 REVIEWED true false 3344 false 3344 true 0364-3190 1573-6903 Journal FOREIGN 47 11 3272 3284 3272 13 2022 Phenylephrine (PE) is a canonical α<jats:sub>1</jats:sub>-adrenoceptor-selective agonist. However, unexpected effects of PE have been observed in preclinical and clinical studies, that cannot be easily explained by its actions on α<jats:sub>1</jats:sub>-adrenoceptors. The probability of the involvement of α<jats:sub>2</jats:sub>- and β-adrenoceptors in the effect of PE has been raised. In addition, our earlier study observed that PE released noradrenaline (NA) in a [Ca<jats:sup>2+</jats:sup>]<jats:sub>o</jats:sub>-independent manner. To elucidate this issue, we have investigated the effects of PE on [<jats:sup>3</jats:sup>H]NA release and α<jats:sub>1</jats:sub>-mediated smooth muscle contractions in the mouse vas deferens (MVD) as ex vivo preparation. The release experiments were designed to assess the effects of PE at the presynaptic terminal, whereas smooth muscle isometric contractions in response to electrical field stimulation were used to measure PE effect postsynaptically. Our results show that PE at concentrations between 0.3 and 30 µM significantly enhanced the resting release of [<jats:sup>3</jats:sup>H]NA in a [Ca<jats:sup>2+</jats:sup>]<jats:sub>o</jats:sub>-independent manner. In addition, prazosin did not affect the release of NA evoked by PE. On the contrary, PE-evoked smooth muscle contractions were inhibited by prazosin administration indicating the α<jats:sub>1</jats:sub>-adrenoceptor-mediated effect. When the function of the NA transporter (NAT) was attenuated with nisoxetine, PE failed to release NA and the contractions were reduced by approximately 88%. The remaining part proved to be prazosin-sensitive. The present work supports the substantial indirect effect of PE which relays on the cytoplasmic release of NA, which might explain the reported side effects for PE. 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(<span class="authorship-author-name">Al-Khrasani Mahmoud</span> <span class="authorAux-mtmt"> Farmakológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Farmakológiai és Farmakoterápiás Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10076883"><a href="/gui2/?type=authors&mode=browse&sel=10076883" target="_blank">Karadi D.A. (<span class="authorship-author-name">Karádi Dávid Árpád</span> <span class="authorAux-mtmt"> Farmakológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Farmakológiai és Farmakoterápiás Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10080867"><a href="/gui2/?type=authors&mode=browse&sel=10080867" target="_blank">Galambos A.R. (<span class="authorship-author-name">Galambos Anna Rita</span> <span class="authorAux-mtmt"> fájdalomkutatás, neuropátia, farmakológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Farmakológiai és Farmakoterápiás Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10008942"><a href="/gui2/?type=authors&mode=browse&sel=10008942" target="_blank">Sperlagh B. 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Szilveszter</span> <span class="authorAux-mtmt"> Neurofarmakológia, neurokémia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Farmakológiai és Farmakoterápiás Intézet; <span title="Kísérleti Orvostudományi Kutatóintézet">KOKI</span>/Molekuláris Farmakológia Kutatócsoport; Kísérleti Orvostudományi Kutatóintézet</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;33062017" target="_blank">The Pharmacological Effects of Phenylephrine are Indirect, Mediated by Noradrenaline Release from the Cytoplasm</a></div> <div> <span class="journal-title">NEUROCHEMICAL RESEARCH</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/0364-3190">0364-3190</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1573-6903">1573-6903</a>)</span>: <span class="journal-volume">47</span> <span class="journal-issue">11</span> <span class="page"> pp 3272-3284 </span> <span class="year">(2022)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="10.1007/s11064-022-03681-2" target="_blank" href="https://doi.org/10.1007/s11064-022-03681-2"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000837966300002" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000837966300002"> WoS </a> </span> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:black" title="160654" target="_blank" href="http://real.mtak.hu/160654"> REAL </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="85135628860" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85135628860"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="35945308" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=35945308&dopt=Abstract"> PubMed </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Folyóirat szakterülete: Scopus - Medicine (miscellaneous)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Biochemistry&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q2</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Cellular and Molecular Neuroscience&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q3</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 19</span> | Független: 15 | Függő: 4 | Nem jelölt: 0 | WoS jelölt: 7 | Scopus jelölt:&nbsp;11 | WoS/Scopus jelölt:&nbsp;11 | DOI jelölt:&nbsp;15 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;33062017&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 15 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 33062017</span> | <span class="status-data status-APPROVED"> Nyilvános </span> Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">Scopus</span> </div> <div class="funder"> (TKP2021-EGA-25), (Open access funding provided by Semmelweis University) </div> <div class="lastModified">Utolsó módosítás: 2025.02.26. 00:12 Ladányi Gusztáv (MTMT API user, admin) </div> <div class="lockedBy">Központi kezelésű 2024.10.17. 18:20 Nemec Évi (MTMT Központi 3, admin) Ladányi Gusztáv (MTMT API user, admin) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Megjegyzés</u>: Department of Pharmacology and Pharmacotherapy, Semmelweis University, Nagyvárad tér 4., Budapest, 1089, Hungary Institute of Experimental Medicine, Eötvös Lóránd Research Network, Szigony utca 43., Budapest, 1083, Hungary Cited By :2 Export Date: 29 August 2024 CODEN: NERED C...</pre> </div></div>