https://m2.mtmt.hu/ hun 2026-05-26 00:02 JournalArticle 33051643 VALIDATED true 0 false 2026-05-22T02:21:57.858+0000 2026-03-26T09:44:49.014+0000 2022-08-16T09:01:54.155+0000 true 10015206 Áncsán Gizella /api/admin/10015206 Admin true 10015206 2026-03-25T17:15:23.282+0000 2026-03-25T17:15:23.282+0000 2024-03-26T13:26:48.956+0000 true 10069305 Kondor Zóra /api/admin/10069305 Admin true true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Kocsis, Dorottya Structural and Functional Analysis of Excised Skins and Human Reconstructed Epidermis with Confocal Raman Spectroscopy and in Microfluidic Diffusion Chambers true true /api/journal/10022148 REVIEWED true false 10022148 false 10022148 true 1999-4923 Journal FOREIGN 14 8 1689 1689 17 2022 Several ex vivo and in vitro skin models are available in the toolbox of dermatological and cosmetic research. Some of them are widely used in drug penetration testing. The excised skins show higher variability, while the in vitro skins provide more reproducible data. The aim of the current study was to compare the chemical composition of different skin models (excised rat skin, excised human skin and human-reconstructed epidermis) by measurement of ceramides, cholesterol, lactate, urea, protein and water at different depths of the tissues. The second goal was to compile a testing system, which includes a skin-on-a-chip diffusion setup and a confocal Raman spectroscopy for testing drug diffusion across the skin barrier and accumulation in the tissue models. A hydrophilic drug caffeine and the P-glycoprotein substrate quinidine were used in the study as topical cream formulations. The results indicate that although the transdermal diffusion of quinidine is lower, the skin accumulation was comparable for the two drugs. The various skin models showed different chemical compositions. The human skin was abundant in ceramides and cholesterol, while the reconstructed skin contained less water and more urea and protein. Based on these results, it can be concluded that skin-on-a-chip and confocal Raman microspectroscopy are suitable for testing drug penetration and distribution at different skin layers within an exposition window. Furthermore, obese human skin should be treated with caution for skin absorption testing due to its unbalanced composition. Funding 2017779 /api/funding/2017779 false true true 2022 true true true false true false GOLD 2026-05-22 true https://doi.org/10.3390/pharmaceutics14081689 19 0 19 16 3 18 18 17 17 17 17 15 15 16 3 0 19 16 19 16 0 7 7 Folyóiratcikk Journal Article 24 18 Könyvrészlet Chapter in Book 25 1 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2023 0 10 8 10 8 2024 0 5 4 5 4 2025 0 3 3 3 3 2026 0 1 1 1 1 Q1 false 10044620,10080212 false false false 2022-09-14T11:22:00.422+0000 Gyógyszerészeti Intézet (SE / GYTK); Információs Technológiai és Bionikai Kar (PPKE); Roska Tamás Műszaki és Természettudományi Dokto... 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0.14285715 false false false Révész Zsolt true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 103134846 /api/authorship/103134846 6 0.14285715 false false false Author 10020626 /api/author/10020626 Antal István true 10020626 true Antal Istvan true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 103134847 /api/authorship/103134847 7 0.143 false true true Author 10044620 /api/author/10044620 Erdő Franciska DUPLUM true 10044620 true Erdő Franciska true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PublicationIdentifier 21582058 /api/publicationidentifier/21582058 PlainSource 6 /api/publicationsource/6 PublicationSourceType 10001 /api/publicationsourcetype/10001 true true true DOI DOI https://doi.org/@@@ true true 6 true GOLD true IDENTICAL 10.3390/pharmaceutics14081689 https://doi.org/10.3390/pharmaceutics14081689 false true PublicationIdentifier 21682301 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<span class="authorAux-mtmt"> biológia</span>) </a> </span> <span class="author-affil"><span title="Pázmány Péter Katolikus Egyetem">PPKE</span>/<span title="Információs Technológiai és Bionikai Kar">ITK</span>/Roska Tamás Műszaki és Természettudományi Doktori Iskola</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Kichou Hichem </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Döme Katalin </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10067129"><a href="/gui2/?type=authors&mode=browse&sel=10067129" target="_blank">Varga-Medveczky Zsófia (<span class="authorship-author-name">Varga-Medveczky Zsófia</span> <span class="authorAux-mtmt"> Biológiai tudományok</span>) </a> </span> <span class="author-affil"><span title="Pázmány Péter Katolikus Egyetem">PPKE</span>/<span title="Információs Technológiai és Bionikai Kar">ITK</span>/Roska Tamás Műszaki és Természettudományi Doktori Iskola</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Révész Zsolt </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10020626"><a href="/gui2/?type=authors&mode=browse&sel=10020626" target="_blank">Antal Istvan (<span class="authorship-author-name">Antal István</span> <span class="authorAux-mtmt"> Gyógyszertechnológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Gyógyszerésztudományi Kar">GYTK</span>/Gyógyszerészeti Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10044620"><a href="/gui2/?type=authors&mode=browse&sel=10044620" target="_blank">Erdő Franciska ✉ (<span class="authorship-author-name">Erdő Franciska</span> <span class="authorAux-mtmt"> biológia, neurodegenerativ betegségek, gyógysze...</span>) </a> </span> <span class="author-affil"><span title="Pázmány Péter Katolikus Egyetem">PPKE</span>/Információs Technológiai és Bionikai Kar</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;33051643" target="_blank">Structural and Functional Analysis of Excised Skins and Human Reconstructed Epidermis with Confocal Raman Spectroscopy and in Microfluidic Diffusion Chambers</a></div> <div> <span class="journal-title">PHARMACEUTICS</span> <span class="journal-issn">( <a target="_blank" href="https://portal.issn.org/resource/ISSN/1999-4923">1999-4923</a>)</span>: <span class="journal-volume">14</span> <span class="journal-issue">8</span> <span class="page"> Paper 1689. 17 p. </span> <span class="year">(2022)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="10.3390/pharmaceutics14081689" target="_blank" href="https://doi.org/10.3390/pharmaceutics14081689"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000847195200001" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000847195200001"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="85137394097" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85137394097"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="36015315" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=36015315&dopt=Abstract"> PubMed </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Folyóirat szakterülete: Scopus - Pharmaceutical Science&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 19</span> | Független: 16 | Függő: 3 | Nem jelölt: 0 | WoS jelölt: 17 | Scopus jelölt:&nbsp;17 | WoS/Scopus jelölt:&nbsp;17 | DOI jelölt:&nbsp;18 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;33051643&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 7 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 33051643</span> | <span class="status-data status-VALIDATED"> Egyeztetett </span> Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">kézi felvitel</span> </div> <div class="funder"> Thematic Excellence Program(TKP2021-EGA-42) </div> <div class="lastModified">Utolsó módosítás: 2026.03.26. 10:44 Pécsi Éva (MTMT Közp 2,3, admin) </div> </div></div>