Diet-Induced Hypercholesterolemia Leads to Cardiac Dysfunction and Alterations in the Myocardial Proteome

Szabó, Márton Richárd [Szabó, Márton Richárd (biokémia), szerző] Biokémiai Intézet (SZTE / SZAOK); Pipicz, Márton [Pipicz, Márton (experimentális ka...), szerző] Biokémiai Intézet (SZTE / SZAOK); Sárközy, Márta [Sárközy, Márta (Elméleti orvostud...), szerző] Biokémiai Intézet (SZTE / SZAOK); Bruszel, Bella [Bruszel, Bella (Analitikai kémia), szerző] Orvosi Vegytani Intézet (SZTE / SZAOK); Szabó, Zoltán [Szabó, Zoltán (proteomika), szerző] Orvosi Vegytani Intézet (SZTE / SZAOK); Csont, Tamás ✉ [Csont, Tamás Bálint (Kísérletes kardio...), szerző] Biokémiai Intézet (SZTE / SZAOK)

Angol nyelvű Szakcikk (Folyóiratcikk) Tudományos
Megjelent: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 1661-6596 1422-0067 23 (13) Paper: 7387 , 17 p. 2022
  • SJR Scopus - Inorganic Chemistry: D1
Azonosítók
Támogatások:
  • (TKP2021-EGA-32) Támogató: NKFIH
  • EMBERI ERŐFORRÁS FEJLESZTÉSI OPERATÍV PROGRAM (EFOP)(EFOP-3.6.2-162017-00006) Támogató: EFOP
Elevated blood cholesterol is a major risk factor for coronary heart disease. Moreover, direct effects on the myocardium also contribute to the adverse effects of hypercholesterolemia. Here, we investigated the effect of hypercholesterolemia on the cardiac proteome. Male Wistar rats were fed with a laboratory rodent chow supplemented with 2% cholesterol for 8 weeks to induce hypercholesterolemia. The protein expression data obtained from the proteomic characterization of left ventricular samples from normo- and hypercholesterolemic animals were subjected to gene ontology (GO) and protein interaction analyses. Elevated circulating cholesterol levels were accompanied by diastolic dysfunction in cholesterol-fed rats. The proteomic characterization of left ventricular samples revealed altered expression of 45 proteins due to hypercholesterolemia. Based on the Gene Ontology analysis, hypercholesterolemia was associated with disturbed expression of cytoskeletal and contractile proteins. Beta-actin was downregulated in the hypercholesterolemic myocardium, and established a prominent hub of the protein interaction network. Analysis of the unfiltered dataset revealed concordant downregulated expression patterns in proteins associated with the arrangement of the contractile system (e.g., cardiac-specific troponins and myosin complex), and in subunits of the mitochondrial respiratory chain. We conclude that the observed changes in the cardiac proteome may contribute to the development of diastolic dysfunction in hypercholesterolemia.
Hivatkozás stílusok: IEEEACMAPAChicagoHarvardCSLMásolásNyomtatás
2025-01-03 17:24