(NKFIH FK 124147 National Research, Development and Innovation Office (NRDIO) – Hungary)
(137749) Támogató: NKFIH
(Bolyai János Kutatási Ösztöndíj) Támogató: MTA
(UNKP-20-5-SE-9)
European network for advancing electro- magnetic hyperthermic medical technologies(COST
CA17115)
(Higher Education Institutional Excellence Program of the Ministry of Human Resources
in Hungary, within the framework of the Therapeutic Development thematic program of
Semmelweis University)
Establishing the Hungarian Center of Excellence for Molecular Medicine in partnership
with EMBL(739593) Támogató: Horizon 2020
Szakterületek:
Tudomány
When exposed to an alternating magnetic field, superparamagnetic nanoparticles can
elicit the required hyperthermic effect while also being excellent magnetic resonance
imaging (MRI) contrast agents. Their main drawback is that they diffuse out of the
area of interest in one or two days, thus preventing a continuous application during
the typical several-cycle multi-week treatment. To solve this issue, our aim was to
synthesise an implantable, biodegradable membrane infused with magnetite that enabled
long-term treatment while having adequate MRI contrast and hyperthermic capabilities.
To immobilise the nanoparticles inside the scaffold, they were synthesised inside
hydrogel fibres. First, polysuccinimide (PSI) fibres were produced by electrospinning
and crosslinked, and then, magnetitc iron oxide nanoparticles (MIONs) were synthesised
inside and in-between the fibres of the hydrogel membranes with the well-known co-precipitation
method. The attenuated total reflectance Fourier-transform infrared spectroscopy (ATR-FTIR)
investigation proved the success of the chemical synthesis and the presence of iron
oxide, and the superconducting quantum interference device (SQUID) study revealed
their superparamagnetic property. The magnetic hyperthermia efficiency of the samples
was significant. The given alternating current (AC) magnetic field could induce a
temperature rise of 5 degrees C (from 37 degrees C to 42 degrees C) in less than 2
min even for five quick heat-cool cycles or for five consecutive days without considerable
heat generation loss in the samples. Short-term (1 day and 7 day) biocompatibility,
biodegradability and MRI contrast capability were investigated in vivo on Wistar rats.
The results showed excellent MRI contrast and minimal acute inflammation.
