Immunochemistry (immunocytochemistry for cells and immunohistochemistry for tissues)
is a method used to label specific antigens, based on highly specific antibody-epitope
interactions. The resulting labeling can be visualized and imaged through microscopy
adapted to the type of detection system used (fluorophore, peroxidase, etc.). In the
nervous system, myelin is a complex membrane structure, generated by myelinating glial
cells, which ensheath axons and facilitate electrical conduction. Myelin alteration
has been shown to occur in various neurological diseases, in which it is associated
with functional deficits. Here, we focus on myelin detection by immunofluorescence
using immunochemistry protocols based on antibodies directed against major myelin
proteins.
