Detection of human papilloma virus (HPV) in tissue specimens has been a clinical challenge
since last 2 decades; however, screening for presence of E6/E7 transcripts is regarded
as the gold standard, and it verifies the active HPV infection. Here, we describe
"RNAscope (R) assay" a novel RNA in situ hybridization (ISH) technology; which detects
E6/E7 mRNA of seven high risk HPV subtypes (HPV 16, 18, 31, 33, 35, 52, and 58) in
formalin fixed paraffin embedded (FFPE) tissue samples.
