Various levels of circulating exosomal total-miRNA and miR-210 hypoxamiR in different forms of pregnancy hypertension

Biró, O [Biró, Orsolya Brigitta (prenatális diagno...), author] I. Department of Obsterics and Gynecology (SU / FM / C); Alasztics, B [Alasztics, Bálint (szülészet-nőgyógy...), author] I. Department of Obsterics and Gynecology (SU / FM / C); Molvarec, A [Molvarec, Attila (Szülészet-nőgyógy...), author] I. Department of Obsterics and Gynecology (SU / FM / C); Joó, J [Joó, József Gábor (Szülészet-nőgyógy...), author] I. Department of Obsterics and Gynecology (SU / FM / C); Nagy, B [Nagy, Bálint (Molekuláris genetika), author] Department of Human Genetics (UD); Rigó, J [Rigó, János (Szülészet-nőgyógy...), author] I. Department of Obsterics and Gynecology (SU / FM / C)

English Scientific Article (Journal Article)
Published: PREGNANCY HYPERTENSION 2210-7789 2210-7797 10 pp. 207-212 2017
  • SJR Scopus - Internal Medicine: Q1
    Introduction: Hypertension is a common complication during pregnancy, affecting 10% of pregnant women worldwide. Several microRNA (miRNA) were shown to be involved in hypertensive disorders of pregnancy. In preeclampsia (PE), placental dysfunction causes the enhanced release of extracellular vesicle-derived miRNAs. The hypoxia-sensitive hsa-mir-210 is the most common PE-associated miRNA, but its exosomal profile has not been investigated. Objectives: Our aims were to measure exosomal total-miRNA concentration and to perform expression analysis of circulating exosomal hsa-miR-210 in women affected by chronic hypertension (CHT) gestational hypertension (GHT) or PE. Materials and methods: We collected plasma samples from women with CHT, GHT, PE (moderate: mPE and severe: sPE) and from normotensive pregnancies. Exosomal miRNAs were extracted and miRNA concentration was measured. RT-PCR was carried out with hsa-miR-210-3p-specific primers and relative expression was calculated using the comparative Ct method. Results: The total-miRNA concentration was different in the disease subgroups, and was significantly higher in mPE and sPE compared to the other groups. We found a significant difference in the relative exosomal hsa-miR-210-3p expression between all hypertensive groups compared to the normotensive samples, but significant upregulation was only observed in case of mPE and sPE patients. Both the level of total-miRNA and hsa-miR-210 expression was higher in case of severe PE. Conclusions: The level of circulating exosomal total-miRNA and hsa-miR-210 was elevated in women with PE, and it was higher in the severe form. We showed that hsa-miR-210 is secreted via exosomes, which may have a role in the pathomechanism of the disease. © 2017 International Society for the Study of Hypertension in Pregnancy.
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    2020-12-04 05:57