Blue Native polyacrylamide gel electrophoresis (BN PAGE) followed by denaturing PAGE
is a widely used, convenient and time efficient method to separate thylakoid complexes
and study their composition, abundance, and interactions. Previous analyses unravelled
multiple monomeric and dimeric/oligomeric thylakoid complexes but, in certain cases,
the separation of complexes was not proper. Particularly, the resolution of super-
and megacomplexes, which provides important information on functional interactions,
still remained challenging.
