The combination of genetic code expansion (GCE) and baculovirus-based protein expression
in Spodoptera frugiperda cells is a powerful tool to express multiprotein complexes
with site-specifically introduced non-canonical amino acids. This protocol describes
the integration of synthetase and tRNA gene indispensable for GCE into the backbone
of the Bacmid, the Tn7-mediated transposition of various genes of interest, as well
as the final expression of protein using the MultiBacTAG system with different noncanonical
amino acids.
