Biológia (elméleti, matematikai, hőbiológia, kriobiológia, biológiai ritmus), evolúciós
biológia
Biológiai tudományok
Mikrobiológia
Orvos- és egészségtudomány
Orvosi mikrobiológia
Periodontitis is caused by pathogenic subgingival microbial biofilm development and
dysbiotic interactions between host and hosted microbes. A thorough characterization
of the subgingival biofilms by deep amplicon sequencing of 121 individual periodontitis
pockets of nine patients and whole metagenomic analysis of the saliva microbial community
of the same subjects were carried out. Two biofilm sampling methods yielded similar
microbial compositions. Taxonomic mapping of all biofilms revealed three distinct
microbial clusters. Two clinical diagnostic parameters, probing pocket depth (PPD)
and clinical attachment level (CAL), correlated with the cluster mapping. The dysbiotic
microbiomes were less diverse than the apparently healthy ones of the same subjects.
The most abundant periodontal pathogens were also present in the saliva, although
in different representations. The single abundant species Tannerella forsythia was
found in the diseased pockets in about 16–17-fold in excess relative to the clinically
healthy sulcus, making it suitable as an indicator of periodontitis biofilms. The
discrete microbial communities indicate strong selection by the host immune system
and allow the design of targeted antibiotic treatment selective against the main periodontal
pathogen(s) in the individual patients.
