https://m2.mtmt.hu/ hun 2026-09-23 10:35 JournalArticle 32480183 VALIDATED true Institute of Enzymology, Research Centre for Natural Sciences, Budapest, Hungary School of Ph.D. Studies, Semmelweis University, Budapest, Hungary Gene Design Ltd., Szeged, Hungary Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary Biospiral-2006 Ltd., Szeged, Hungary School of Ph.D. Studies, University of Szeged, Szeged, Hungary Institute of Biochemistry, Biological Research Centre, Szeged, Hungary Export Date: 23 August 2025; Cited By: 14; Correspondence Address: A. Tálas; Institute of Enzymology, Research Centre for Natural Sciences, Budapest, Hungary; email: talas.andras@ttk.hu; E. Welker; Institute of Enzymology, Research Centre for Natural Sciences, Budapest, Hungary; email: welker.ervin@ttk.hu 0 false 2026-09-11T13:31:56.120+0000 2025-08-25T08:43:35.593+0000 2021-11-08T07:23:20.238+0000 true 10078525 Varga Éva /api/author/10078525 Author Varga Éva (Géntechnológia, biológia) true 2025-08-25T08:42:44.604+0000 2025-08-25T08:42:44.604+0000 2025-08-25T08:43:35.781+0000 true 10097128 Sass Dávid /api/admin/10097128 Admin Sass Dávid (SZTE admin5) true true false true 24 24 /api/publicationtype/24 PublicationType Folyóiratcikk 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType Folyóiratcikk 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType Szakcikk (Folyóiratcikk) 101 true 10000059 true 1 /api/category/1 Category Tudományos true 1 Tálas, András true true /api/journal/10015678 REVIEWED NATURE COMMUNICATIONS 2041-1723 true false 10015678 false 10015678 true 2041-1723 Journal FOREIGN 12 1 6353 6353 14 2021 Adenine and cytosine base editors (ABE, CBE) allow for precision genome engineering. Here, Base Editor Activity Reporter (BEAR), a plasmid-based fluorescent tool is introduced, which can be applied to report on ABE and CBE editing in a virtually unrestricted sequence context or to label base edited cells for enrichment. Using BEAR-enrichment, we increase the yield of base editing performed by nuclease inactive base editors to the level of the nickase versions while maintaining significantly lower indel background. Furthermore, by exploiting the semi-high-throughput potential of BEAR, we examine whether increased fidelity SpCas9 variants can be used to decrease SpCas9-dependent off-target effects of ABE and CBE. Comparing them on the same target sets reveals that CBE remains active on sequences, where increased fidelity mutations and/or mismatches decrease the activity of ABE. Our results suggest that the deaminase domain of ABE is less effective to act on rather transiently separated target DNA strands, than that of CBE explaining its lower mismatch tolerance. Funding 2010070 /api/funding/2010070 (K128188) false true Funding 2010071 /api/funding/2010071 (K134968) false true Funding 2010072 /api/funding/2010072 (PD134858) Támogató: OTKA false true Funding 2010073 /api/funding/2010073 (2018-1.1.1-MKI-2018-00167) false true Funding 2010074 /api/funding/2010074 (ÚNKP-20-5-SE-20) Támogató: ÚNKP false true Funding 2010075 /api/funding/2010075 (VEKOP-2.1.7-15-2016-00393) false true Funding 2010076 /api/funding/2010076 (BO/764/20) Támogató: Bolyai János Kutatási Ösztöndíj false true Funding 2010077 /api/funding/2010077 Az orvos-, egészségtudományi- és gyógyszerészképzés tudományos műhelyeinek fejlesztése(EFOP-3.6.3-VEKOP-16-2017-00009) Támogató: EFOP-VEKOP false true true true 2021 true true true false true false GOLD 2026-09-11 true https://doi.org/10.1038/s41467-021-26461-y 20 0 20 13 6 20 20 18 19 20 20 13 13 13 7 0 20 13 20 13 0 4 4 Folyóiratcikk Journal Article 24 19 Könyvrészlet Chapter in Book 25 1 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2022 0 3 1 3 1 2023 0 6 4 6 4 2024 0 3 3 3 3 2025 0 5 4 5 4 2026 0 3 1 3 1 D1 A nemzetközi false 10001345,10055848,10038471 false false false 2021-11-24T07:40:41.455+0000 Biofizikai és Sugárbiológiai Intézet (SE / AOK / I); Biokémiai Intézet (HRN SZBK); Doktori Iskola (SE); Enzimológiai Intézet (TTK); Multidiszciplináris Orvostudományok Doktori Iskola (SZTE / DI) 6 26 5 6 0 true Language 10002 /api/language/10002 Angol Angol English true 2 true PersonAuthorship 99297070 /api/authorship/99297070 Tálas, András ✉ [Tálas, András (Molekuláris Biológus), szerző] Enzimológiai Intézet (TTK); Doktori Iskola (SE) 1 0.167 true false true Author 10055848 /api/author/10055848 Tálas András (Molekuláris Biológus) Tálas András true 10055848 true Tálas András true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 99297071 /api/authorship/99297071 Simon, Dorottya A. [Simon, Dorottya Anna (géntechnológia), szerző] Enzimológiai Intézet (TTK) 2 0.167 false false false Author 10081107 /api/author/10081107 Simon Dorottya Anna (géntechnológia) Simon Dorottya Anna true true Simon Dorottya A. true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 99297072 /api/authorship/99297072 Kulcsár, Péter I. [Kulcsár, Péter István (Molekuláris biológia), szerző] Biofizikai és Sugárbiológiai Intézet (SE / AOK / I) 3 0.167 false false false Author 10038471 /api/author/10038471 Kulcsár Péter István (Molekuláris biológia) Kulcsár Péter István true 10038471 true Kulcsár Péter I. true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 99297073 /api/authorship/99297073 Varga, Éva [Varga, Éva (Géntechnológia, b...), szerző] Multidiszciplináris Orvostudományok Doktori Iskola (SZTE / DI) 4 0.167 false false false Author 10078525 /api/author/10078525 Varga Éva (Géntechnológia, biológia) Varga Éva true true Varga Éva true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 99297074 /api/authorship/99297074 Krausz, Sarah L. [Krausz, Sarah (géntechnológia), szerző] Doktori Iskola (SE) 5 0.167 false false false Author 10061281 /api/author/10061281 Krausz Sarah (géntechnológia) Krausz Sarah true 10061281 true Krausz Sarah L. true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 99297075 /api/authorship/99297075 Welker, Ervin ✉ [Welker, Ervin (Fehérje feltekeredés), szerző] Biokémiai Intézet (HRN SZBK); Enzimológiai Intézet (TTK) 6 0.167 false true true Author 10001345 /api/author/10001345 Welker Ervin (Fehérje feltekeredés) Welker Ervin true 10001345 true Welker Ervin true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PublicationIdentifier 20151625 /api/publicationidentifier/20151625 DOI: 10.1038/s41467-021-26461-y PlainSource 6 /api/publicationsource/6 DOI PublicationSourceType 10001 /api/publicationsourcetype/10001 DOI true true true DOI DOI https://doi.org/@@@ true true 6 true GOLD true IDENTICAL 10.1038/s41467-021-26461-y https://doi.org/10.1038/s41467-021-26461-y false true PublicationIdentifier 20199650 /api/publicationidentifier/20199650 WoS: 000714451200028 PlainSource 1 /api/publicationsource/1 WoS PublicationSourceType 10003 /api/publicationsourcetype/10003 Indexelő adatbázis false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true IDENTICAL 000714451200028 https://www.webofscience.com/wos/woscc/full-record/000714451200028 false true PublicationIdentifier 20544068 /api/publicationidentifier/20544068 REAL: 137510 SwordSource 36 /api/publicationsource/36 REAL PublicationSourceType 10007 /api/publicationsourcetype/10007 Repozitórium true true true REAL REAL https://real.mtak.hu/@@@ true true 36 true GOLD true 137510 https://real.mtak.hu/137510 false true PublicationIdentifier 20199649 /api/publicationidentifier/20199649 Scopus: 85118547578 PlainSource 3 /api/publicationsource/3 Scopus PublicationSourceType 10003 /api/publicationsourcetype/10003 Indexelő adatbázis false true true Scopus Scopus http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@ true true 3 true IDENTICAL 85118547578 http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85118547578 false true PublicationIdentifier 20223699 /api/publicationidentifier/20223699 PubMed: 34732717 PlainSource 17 /api/publicationsource/17 PubMed PublicationSourceType 10003 /api/publicationsourcetype/10003 Indexelő adatbázis false true true PubMed PubMed http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=@@@&dopt=Abstract true true 17 true IDENTICAL 34732717 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=34732717&dopt=Abstract false true MtaRating 11551153 /api/mtarating/11551153 IX. Regionális Tudományok Bizottsága:A nemzetközi NATURE COMMUNICATIONS 2041-1723 RatingType 10010 /api/ratingtype/10010 IX. Regionális Tudományok Bizottsága RegTB Institute 14100 /api/institute/14100 Regionális Tudományok Bizottsága IXGJO RTB [1901-] 0 false true false Regionális Tudományok Bizottsága Committee on Regional Studies true 14100 true true true A nemzetközi false true SjrRating 11195759 /api/sjrrating/11195759 sjr:D1 (2021) Scopus - Biochemistry, Genetics and Molecular Biology (miscellaneous) NATURE COMMUNICATIONS 2041-1723 9 0.1 journal RatingType 10002 /api/ratingtype/10002 sjr sjr true true ClassificationExternal 1301 /api/classificationexternal/1301 Scopus - Biochemistry, Genetics and Molecular Biology (miscellaneous) true 1301 true D1 DIRECT true true Reference 24921646 /api/reference/24921646 1. Jinek, M. et al. A programmable dual-RNA–guided DNA endonuclease in adaptive bacterial immunity. Science 337, 816–821 (2012)., DOI: 10.1126/science.1225829 1 10.1126/science.1225829 false true Reference 24921647 /api/reference/24921647 2. Mali, P. et al. RNA-guided human genome engineering via Cas9. Science 339, 823–826 (2013)., DOI: 10.1126/science.1232033 2 10.1126/science.1232033 false true Reference 24921648 /api/reference/24921648 3. Cong, L. et al. Multiplex genome engineering using CRISPR/Cas systems. Science 339, 819–823 (2013)., DOI: 10.1126/science.1231143 3 10.1126/science.1231143 false true Reference 24921649 /api/reference/24921649 4. Gasiunas, G., Barrangou, R., Horvath, P. & Siksnys, V. Cas9–crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria. Proc. Natl Acad. Sci. USA 109, E2579–E2586 (2012)., DOI: 10.1073/pnas.1208507109 4 10.1073/pnas.1208507109 false true Reference 24921650 /api/reference/24921650 5. Cullot, G. et al. CRISPR-Cas9 genome editing induces megabase-scale chromosomal truncations. Nat. Commun. 10, 1136 (2019)., DOI: 10.1038/s41467-019-09006-2 5 10.1038/s41467-019-09006-2 false true Reference 24921651 /api/reference/24921651 6. Tsai, S. Q. et al. GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases. Nat. Biotechnol. 33, 187–197 (2015)., DOI: 10.1038/nbt.3117 6 10.1038/nbt.3117 false true Reference 24921652 /api/reference/24921652 7. Komor, A. C., Kim, Y. B., Packer, M. S., Zuris, J. A. & Liu, D. R. Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature 533, 420–424 (2016)., DOI: 10.1038/nature17946 7 10.1038/nature17946 false true Reference 24921653 /api/reference/24921653 8. Gaudelli, N. M. et al. Programmable base editing of A• T to G• C in genomic DNA without DNA cleavage. Nature 551, 464–471 (2017)., DOI: 10.1038/nature24644 8 10.1038/nature24644 false true Reference 24921654 /api/reference/24921654 9. Li, X. et al. Base editing with a Cpf1–cytidine deaminase fusion. Nat. Biotechnol. 36, 324–327 (2018)., DOI: 10.1038/nbt.4102 9 10.1038/nbt.4102 false true Reference 24921655 /api/reference/24921655 10. Lee, H. K. et al. Targeting fidelity of adenine and cytosine base editors in mouse embryos. Nat. Commun. 9, 1–6 (2018)., DOI: 10.1038/s41467-017-02088-w 10 10.1038/s41467-017-02088-w false true Reference 24921656 /api/reference/24921656 11. Komor, A. C. et al. Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C: G-to-T: A base editors with higher efficiency and product purity. Sci. Adv. 3, eaao4774 (2017)., DOI: 10.1126/sciadv.aao4774 11 10.1126/sciadv.aao4774 false true Reference 24921657 /api/reference/24921657 12. Coelho, M. A. et al. BE-FLARE: a fluorescent reporter of base editing activity reveals editing characteristics of APOBEC3A and APOBEC3B. BMC Biol. 16, 150 (2018)., DOI: 10.1186/s12915-018-0617-1 12 10.1186/s12915-018-0617-1 false true Reference 24921658 /api/reference/24921658 13. St. Martin, A. et al. A fluorescent reporter for quantification and enrichment of DNA editing by APOBEC–Cas9 or cleavage by Cas9 in living cells. Nucleic Acids Res. 46, e84–e84 (2018)., DOI: 10.1093/nar/gky332 13 10.1093/nar/gky332 false true Reference 24921659 /api/reference/24921659 14. Standage-Beier, K. et al. A transient reporter for editing enrichment (TREE) in human cells. Nucleic Acids Res. 47, e120–e120 (2019)., DOI: 10.1093/nar/gkz713 14 10.1093/nar/gkz713 false true Reference 24921660 /api/reference/24921660 15. Katti, A. et al. GO: a functional reporter system to identify and enrich base editing activity. Nucleic Acids Res. 48, 2841–2852 (2020)., DOI: 10.1093/nar/gkaa124 15 10.1093/nar/gkaa124 false true Reference 24921661 /api/reference/24921661 16. Wang P., Xu L., Gao Y., Han R. BEON: A functional fluorescence reporter for quantification and enrichment of adenine base editing activity. Mol. Ther. 28, 1696–1705 (2020)., DOI: 10.1016/j.ymthe.2020.04.009 16 10.1016/j.ymthe.2020.04.009 false true Reference 24921662 /api/reference/24921662 17. Kim, Y. B. et al. Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat. Biotechnol. 35, 371–376 (2017)., DOI: 10.1038/nbt.3803 17 10.1038/nbt.3803 false true Reference 24921663 /api/reference/24921663 18. Kleinstiver, B. P. et al. Engineered CRISPR–Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat. Biotechnol. 37, 276–282 (2019)., DOI: 10.1038/s41587-018-0011-0 18 10.1038/s41587-018-0011-0 false true Reference 24921664 /api/reference/24921664 19. Tóth, E. et al. Improved LbCas12a variants with altered PAM specificities further broaden the genome targeting range of Cas12a nucleases. Nucleic Acids Res. 48, 3722–3733 (2020)., DOI: 10.1093/nar/gkaa110 19 10.1093/nar/gkaa110 false true Reference 24921665 /api/reference/24921665 20. Gehrke, J. M. et al. An APOBEC3A-Cas9 base editor with minimized bystander and off-target activities. Nat. Biotechnol. 36, 977–982 (2018)., DOI: 10.1038/nbt.4199 20 10.1038/nbt.4199 false true Reference 24921666 /api/reference/24921666 21. Koblan, L. W. et al. Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat. Biotechnol. 36, 843–846 (2018)., DOI: 10.1038/nbt.4172 21 10.1038/nbt.4172 false true Reference 24921667 /api/reference/24921667 22. Zafra, M. P. et al. Optimized base editors enable efficient editing in cells, organoids and mice. Nat. Biotechnol. 36, 888–893 (2018)., DOI: 10.1038/nbt.4194 22 10.1038/nbt.4194 false true Reference 24921668 /api/reference/24921668 23. Grünewald, J. et al. Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors. Nature 569, 433–437 (2019)., DOI: 10.1038/s41586-019-1161-z 23 10.1038/s41586-019-1161-z false true Reference 24921669 /api/reference/24921669 24. Huang, T. P. et al. Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat. Biotechnol. 37, 626–631 (2019)., DOI: 10.1038/s41587-019-0134-y 24 10.1038/s41587-019-0134-y false true Reference 24921670 /api/reference/24921670 25. Tan, J., Zhang, F., Karcher, D. & Bock, R. Engineering of high-precision base editors for site-specific single nucleotide replacement. Nat. Commun. 10, 439 (2019)., DOI: 10.1038/s41467-018-08034-8 25 10.1038/s41467-018-08034-8 false true Reference 24921671 /api/reference/24921671 26. Wang, L. et al. Eliminating base-editor-induced genome-wide and transcriptome-wide off-target mutations. Nat. Cell Biol. 23, 552–563 (2021)., DOI: 10.1038/s41556-021-00671-4 26 10.1038/s41556-021-00671-4 false true Reference 24921672 /api/reference/24921672 27. Anzalone A. V., Koblan L. W., Liu D. R. Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors. Nat. Biotechnol. 38, 824–844 (2020)., DOI: 10.1038/s41587-020-0561-9 27 10.1038/s41587-020-0561-9 false true Reference 24921673 /api/reference/24921673 28. Liang, P. et al. Genome-wide profiling of adenine base editor specificity by EndoV-seq. Nat. Commun. 10, 67 (2019)., DOI: 10.1038/s41467-018-07988-z 28 10.1038/s41467-018-07988-z false true Reference 24921674 /api/reference/24921674 29. Kim, D. et al. Genome-wide target specificities of CRISPR RNA-guided programmable deaminases. Nat. Biotechnol. 35, 475–480 (2017)., DOI: 10.1038/nbt.3852 29 10.1038/nbt.3852 false true Reference 24921675 /api/reference/24921675 30. Kim, D., Kim, D.-E., Lee, G., Cho, S.-I. & Kim, J.-S. Genome-wide target specificity of CRISPR RNA-guided adenine base editors. Nat. Biotechnol. 37, 430–435 (2019)., DOI: 10.1038/s41587-019-0050-1 30 10.1038/s41587-019-0050-1 false true Reference 24921676 /api/reference/24921676 31. Kleinstiver, B. P. et al. High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects. Nature 529, 490–495 (2016)., DOI: 10.1038/nature16526 31 10.1038/nature16526 false true Reference 24921677 /api/reference/24921677 32. Slaymaker, I. M. et al. Rationally engineered Cas9 nucleases with improved specificity. Science 351, 84–88 (2016)., DOI: 10.1126/science.aad5227 32 10.1126/science.aad5227 false true Reference 24921678 /api/reference/24921678 33. Chen, J. S. et al. Enhanced proofreading governs CRISPR–Cas9 targeting accuracy. Nature 550, 407–410 (2017)., DOI: 10.1038/nature24268 33 10.1038/nature24268 false true Reference 24921679 /api/reference/24921679 34. Casini, A. et al. A highly specific SpCas9 variant is identified by in vivo screening in yeast. Nat. Biotechnol. 36, 265–271 (2018)., DOI: 10.1038/nbt.4066 34 10.1038/nbt.4066 false true Reference 24921680 /api/reference/24921680 35. Kulcsár, P. I. et al. Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage. Genome Biol. 18, 1–17 (2017)., DOI: 10.1186/s13059-017-1318-8 35 10.1186/s13059-017-1318-8 false true Reference 24921681 /api/reference/24921681 36. Schmid-Burgk J. L. et al. Highly parallel profiling of Cas9 variant specificity. Mol. Cell 78, 794–800.e8 (2020)., DOI: 10.1016/j.molcel.2020.02.023 36 10.1016/j.molcel.2020.02.023 false true Reference 24921682 /api/reference/24921682 37. Kim N. et al. Prediction of the sequence-specific cleavage activity of Cas9 variants. Nat. Biotechnol. 38, 1328–1336 (2020)., DOI: 10.1038/s41587-020-0537-9 37 10.1038/s41587-020-0537-9 false true Reference 24921683 /api/reference/24921683 38. Kulcsár, P. I. et al. Blackjack mutations improve the on-target activities of increased fidelity variants of SpCas9 with 5′ G-extended sgRNAs. Nat. Commun. 11, 1–14 (2020)., DOI: 10.1038/s41467-020-15021-5 38 10.1038/s41467-020-15021-5 false true Reference 24921684 /api/reference/24921684 39. Kurt I. C. et al. CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells. Nat. Biotechnol. 39, 41–46 (2020)., DOI: 10.1038/s41587-020-0609-x 39 10.1038/s41587-020-0609-x false true Reference 24921685 /api/reference/24921685 40. Martin, A. S. et al. A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes. Sci. Rep. 9, 497 (2019)., DOI: 10.1038/s41598-018-36739-9 40 10.1038/s41598-018-36739-9 false true Reference 24921686 /api/reference/24921686 41. Burset, M., Seledtsov, I. & Solovyev, V. Analysis of canonical and non-canonical splice sites in mammalian genomes. Nucleic acids Res. 28, 4364–4375 (2000)., DOI: 10.1093/nar/28.21.4364 41 10.1093/nar/28.21.4364 false true Reference 24921687 /api/reference/24921687 42. Senapathy P., Shapiro M. B., Harris N. L. Splice junctions, branch point sites, and exons: sequence statistics, identification, and applications to genome project. Methods Enzymol. 183, 252–278 (1990)., DOI: 10.1016/0076-6879(90)83018-5 42 10.1016/0076-6879(90)83018-5 false true Reference 24921688 /api/reference/24921688 43. Kluesner, M. G. et al. EditR: a method to quantify base editing from Sanger sequencing. CRISPR J. 1, 239–250 (2018)., DOI: 10.1089/crispr.2018.0014 43 10.1089/crispr.2018.0014 false true Reference 24921689 /api/reference/24921689 44. Zhao D. et al. Glycosylase base editors enable C-to-A and C-to-G base changes. Nat. Biotechnol. 39, 35–40 (2020)., DOI: 10.1038/s41587-020-0592-2 44 10.1038/s41587-020-0592-2 false true Reference 24921690 /api/reference/24921690 45. Rees, H. A. & Liu, D. R. Base editing: precision chemistry on the genome and transcriptome of living cells. Nat. Rev. Genet. 19, 770–788 (2018)., DOI: 10.1038/s41576-018-0059-1 45 10.1038/s41576-018-0059-1 false true Reference 24921691 /api/reference/24921691 46. Jin, S. et al. Cytosine, but not adenine, base editors induce genome-wide off-target mutations in rice. Science 364, 292–295 (2019)., DOI: 10.1126/science.aaw7166 46 10.1126/science.aaw7166 false true Reference 24921692 /api/reference/24921692 47. Zuo, E. et al. Cytosine base editor generates substantial off-target single-nucleotide variants in mouse embryos. Science 364, 289–292 (2019)., DOI: 10.1126/science.aav9973 47 10.1126/science.aav9973 false true Reference 24921693 /api/reference/24921693 48. Rees, H. A. et al. Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery. Nat. Commun. 8, 1–10 (2017)., DOI: 10.1038/ncomms15790 48 10.1038/ncomms15790 false true Reference 24921694 /api/reference/24921694 49. Liang, P. et al. Effective gene editing by high-fidelity base editor 2 in mouse zygotes. Protein Cell 8, 601–611 (2017)., DOI: 10.1007/s13238-017-0418-2 49 10.1007/s13238-017-0418-2 false true Reference 24921695 /api/reference/24921695 50. Lee, J. K. et al. Directed evolution of CRISPR-Cas9 to increase its specificity. Nat. Commun. 9, 1–10 (2018)., DOI: 10.1038/s41467-017-02088-w 50 10.1038/s41467-017-02088-w false true Reference 24921696 /api/reference/24921696 51. Xu, W. et al. Multiplex nucleotide editing by high-fidelity Cas9 variants with improved efficiency in rice. BMC Plant Biol. 19, 1–10 (2019)., DOI: 10.1186/s12870-018-1600-2 51 10.1186/s12870-018-1600-2 false true Reference 24921697 /api/reference/24921697 52. Song M. et al. Sequence-specific prediction of the efficiencies of adenine and cytosine base editors. Nat. Biotechnol. 38, 1037–1043 (2020)., DOI: 10.1038/s41587-020-0573-5 52 10.1038/s41587-020-0573-5 false true Reference 24921698 /api/reference/24921698 53. Wu, X. et al. Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells. Nat. Biotechnol. 32, 670–676 (2014)., DOI: 10.1038/nbt.2889 53 10.1038/nbt.2889 false true Reference 24921699 /api/reference/24921699 54. Richter M. F. et al. Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity. Nat. Biotechnol. 38, 883–891 (2020)., DOI: 10.1038/s41587-020-0453-z 54 10.1038/s41587-020-0453-z false true Reference 24921700 /api/reference/24921700 55. Lapinaite, A. et al. DNA capture by a CRISPR-Cas9–guided adenine base editor. Science 369, 566–571 (2020)., DOI: 10.1126/science.abb1390 55 10.1126/science.abb1390 false true Reference 24921701 /api/reference/24921701 56. Arbab M. et al. Determinants of base editing outcomes from target library analysis and machine learning. Cell182, 463–480.e30 (2020)., DOI: 10.1016/j.cell.2020.05.037 56 10.1016/j.cell.2020.05.037 false true Reference 24921702 /api/reference/24921702 57. Okafor, I. C. et al. Single molecule analysis of effects of non-canonical guide RNAs and specificity-enhancing mutations on Cas9-induced DNA unwinding. Nucleic Acids Res. 47, 11880–11888 (2019). 57 false true Reference 24921703 /api/reference/24921703 58. Sternberg, S. H., LaFrance, B., Kaplan, M. & Doudna, J. A. Conformational control of DNA target cleavage by CRISPR–Cas9. Nature 527, 110–113 (2015)., DOI: 10.1038/nature15544 58 10.1038/nature15544 false true Reference 24921704 /api/reference/24921704 59. Sternberg, S. H., Redding, S., Jinek, M., Greene, E. C. & Doudna, J. A. DNA interrogation by the CRISPR RNA-guided endonuclease Cas9. Nature 507, 62–67 (2014)., DOI: 10.1038/nature13011 59 10.1038/nature13011 false true Reference 24921705 /api/reference/24921705 60. Dagdas, Y. S., Chen, J. S., Sternberg, S. H., Doudna, J. A. & Yildiz, A. A conformational checkpoint between DNA binding and cleavage by CRISPR-Cas9. Sci. Adv. 3, eaao0027 (2017)., DOI: 10.1126/sciadv.aao0027 60 10.1126/sciadv.aao0027 false true Reference 24921706 /api/reference/24921706 61. Sung, K., Park, J., Kim, Y., Lee, N. K. & Kim, S. K. Target specificity of Cas9 nuclease via DNA rearrangement regulated by the REC2 domain. J. Am. Chem. Soc. 140, 7778–7781 (2018)., DOI: 10.1021/jacs.8b03102 61 10.1021/jacs.8b03102 false true Reference 24921707 /api/reference/24921707 62. Anzalone, A. V. et al. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature 576, 149–157 (2019)., DOI: 10.1038/s41586-019-1711-4 62 10.1038/s41586-019-1711-4 false true Reference 24921708 /api/reference/24921708 63. Bindels, D. S. et al. mScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nat. methods 14, 53–56 (2017)., DOI: 10.1038/nmeth.4074 63 10.1038/nmeth.4074 false true Reference 24921709 /api/reference/24921709 64. Tálas, A. et al. A convenient method to pre-screen candidate guide RNAs for CRISPR/Cas9 gene editing by NHEJ-mediated integration of a ‘self-cleaving’GFP-expression plasmid. DNA Res. 24, 609–621 (2017)., DOI: 10.1093/dnares/dsx029 64 10.1093/dnares/dsx029 false true Reference 24921710 /api/reference/24921710 65. Richardson, C. D., Ray, G. J., DeWitt, M. A., Curie, G. L. & Corn, J. E. Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA. Nat. Biotechnol. 34, 339–344 (2016)., DOI: 10.1038/nbt.3481 65 10.1038/nbt.3481 false true /api/publication/32480183 Tálas András et al. BEAR reveals that increased fidelity variants can successfully reduce the mismatch tolerance of adenine but not cytosine base editors. (2021) NATURE COMMUNICATIONS 2041-1723 12 1<div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10055848"><a href="/gui2/?type=authors&mode=browse&sel=10055848" target="_blank">Tálas András ✉ (<span class="authorship-author-name">Tálas András</span> <span class="authorAux-mtmt"> Molekuláris Biológus</span>) </a> </span> <span class="author-affil"><span title="Természettudományi Kutatóközpont">TTK</span>/Enzimológiai Intézet; <span title="Semmelweis Egyetem">SE</span>/Doktori Iskola</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10081107"><a href="/gui2/?type=authors&mode=browse&sel=10081107" target="_blank">Simon Dorottya A. (<span class="authorship-author-name">Simon Dorottya Anna</span> <span class="authorAux-mtmt"> géntechnológia</span>) </a> </span> <span class="author-affil"><span title="Természettudományi Kutatóközpont">TTK</span>/Enzimológiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10038471"><a href="/gui2/?type=authors&mode=browse&sel=10038471" target="_blank">Kulcsár Péter I. (<span class="authorship-author-name">Kulcsár Péter István</span> <span class="authorAux-mtmt"> Molekuláris biológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Biofizikai és Sugárbiológiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10078525"><a href="/gui2/?type=authors&mode=browse&sel=10078525" target="_blank">Varga Éva (<span class="authorship-author-name">Varga Éva</span> <span class="authorAux-mtmt"> Géntechnológia, biológia</span>) </a> </span> <span class="author-affil"><span title="Szegedi Tudományegyetem">SZTE</span>/<span title="Doktori Iskolák">DI</span>/Multidiszciplináris Orvostudományok Doktori Iskola</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10061281"><a href="/gui2/?type=authors&mode=browse&sel=10061281" target="_blank">Krausz Sarah L. (<span class="authorship-author-name">Krausz Sarah</span> <span class="authorAux-mtmt"> géntechnológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/Doktori Iskola</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10001345"><a href="/gui2/?type=authors&mode=browse&sel=10001345" target="_blank">Welker Ervin ✉ (<span class="authorship-author-name">Welker Ervin</span> <span class="authorAux-mtmt"> Fehérje feltekeredés</span>) </a> </span> <span class="author-affil"><span title="Szegedi Biológiai Kutatóközpont">SZBK</span>/Biokémiai Intézet; <span title="Természettudományi Kutatóközpont">TTK</span>/Enzimológiai Intézet</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;32480183" target="_blank">BEAR reveals that increased fidelity variants can successfully reduce the mismatch tolerance of adenine but not cytosine base editors</a></div> <div> <span class="journal-title">NATURE COMMUNICATIONS</span> <span class="journal-issn">( <a target="_blank" href="https://portal.issn.org/resource/ISSN/2041-1723">2041-1723</a>)</span>: <span class="journal-volume">12</span> <span class="journal-issue">1</span> <span class="page"> Paper 6353. 14 p. </span> <span class="year">(2021)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="10.1038/s41467-021-26461-y" target="_blank" href="https://doi.org/10.1038/s41467-021-26461-y"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000714451200028" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000714451200028"> WoS </a> </span> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:black" title="137510" target="_blank" href="https://real.mtak.hu/137510"> REAL </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="85118547578" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85118547578"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="34732717" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=34732717&dopt=Abstract"> PubMed </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Folyóirat szakterülete: Scopus - Biochemistry, Genetics and Molecular Biology (miscellaneous)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Chemistry (miscellaneous)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Physics and Astronomy (miscellaneous)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> <div class="journal-subject">Regionális Tudományok Bizottsága IXGJO RTB [1901-]&nbsp;&nbsp;&nbsp;A nemzetközi</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 20</span> | Független: 13 | Függő: 7 | Nem jelölt: 0 | WoS jelölt: 18 | Scopus jelölt:&nbsp;19 | WoS/Scopus jelölt:&nbsp;20 | DOI jelölt:&nbsp;20 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;32480183&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 4 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 32480183</span> | <span class="status-data status-VALIDATED"> Egyeztetett </span> Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">kézi felvitel</span> </div> <div class="funder"> (K128188), (K134968), (PD134858) Támogató: OTKA, (2018-1.1.1-MKI-2018-00167), (ÚNKP-20-5-SE-20) Támogató: ÚNKP, (VEKOP-2.1.7-15-2016-00393), (BO/764/20) Támogató: Bolyai János Kutatási Ösztöndíj, Az orvos-, egészségtudományi- és gyógyszerészképzés tudományos műhelyeinek fejlesztése(EFOP-3.6.3-VEKOP-16-2017-00009) Támogató: EFOP-VEKOP </div> <div class="lastModified">Utolsó módosítás: 2025.08.25. 10:43 Sass Dávid (SZTE admin5) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Megjegyzés</u>: Institute of Enzymology, Research Centre for Natural Sciences, Budapest, Hungary School of Ph.D. Studies, Semmelweis University, Budapest, Hungary Gene Design Ltd., Szeged, Hungary Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary Biospiral-2006 Ltd., Szeged, Hu...</pre> </div></div>