Intelligens szakosodást szolgáló fejlesztések az Állatorvostudományi Egyetem és a
Széchenyi Istvá...(EFOP-3.6.1-16-2016-00024) Funder: EFOP
Subjects:
Veterinary science
Background: Chlorine dioxide (ClO2) is an inorganic, potent biocide and is available
in highly purified aqueous solution. It can be administered as an oral antiseptic
in this form. Objectives: Our aim is to determine the level of inflammatory markers
and cytochrome genes expressed by enterocytes exposed to different concentrations
of hyperpure chlorine dioxide solution. Methods: Porcine jejunal enterocyte cell (IPEC-J2)
cultures were treated with the aqueous solution of hyper-pure chlorine dioxide of
various concentrations. We determined the alterations in mRNA levels of inflammatory
mediators, such as IL6, CXCL8/IL8, TNF, HSPA6 (Hsp70), CAT and PTGS2 (COX2); furthermore,
the expression of three cytochrome genes (CYP1A1, CYP1A2, CYP3A29) were analysed by
quantitative PCR method. Results: The highest applied ClO2 concentration reduced the
expression of all three investigated CYP genes. The gene expression of PTGS2 and CAT
were not altered by most concentrations of ClO2. The expression of IL8 gene was reduced
by all applied concentrations of ClO2. TNF mRNA level was also decreased by most ClO2
concentrations used. Conclusions: Different concentrations of chlorine dioxide exhibited
immunomodulatory activity and caused altered transcription of CYP450 genes in porcine
enterocytes. Further studies are needed to determine the appropriate ClO2 concentration
for oral use in animals.
