FHR-5 Serum Levels and CFHR5 Genetic Variations in Patients With Immune Complex-Mediated Membranoproliferative Glomerulonephritis and C3-Glomerulopathy

Garam, Nóra [Garam, Nóra (PhD hallgató), author] Department of Internal Medicine and Oncology (SU / FM / C); Cserhalmi, Marcell* [Cserhalmi, Marcell (Molekuláris biológia), author] MTA-ELTE Lendület Complement Research Group (ELTE / ELU FoS / Bio_I); Prohászka, Zoltán ✉ [Prohászka, Zoltán (Immunológia), author] MTA-SE Immunology and Hematology Research Group (SU / FM / C / BHK); Belgyógyászati és Hematológiai Klinika (SU / FM / C); Szilágyi, Ágnes [Szilágyi, Ágnes (Humán molekuláris...), author] Belgyógyászati és Hematológiai Klinika (SU / FM / C); Veszeli, Nóra [Veszeli, Nóra (biológus), author] MTA-SE Immunology and Hematology Research Group (SU / FM / C / BHK); Belgyógyászati és Hematológiai Klinika (SU / FM / C); Szabó, Edina [Szabó, Edina Erzsébet (Humán molekuláris...), author] Belgyógyászati és Hematológiai Klinika (SU / FM / C); Uzonyi, Barbara [Uzonyi, Barbara (Immunológia), author] Department of Immunology (ELTE / ELU FoS / Bio_I); Iliás, Attila [Iliás, Attila (molekuláris biológia), author] Department of Immunology (ELTE / ELU FoS / Bio_I); Aigner, Christof; Schmidt, Alice; Gaggl, Martina; Sunder-Plassmann, Gere; Bajcsi, Dóra [Bajcsi, Dóra (nefrológia), author] First Department of Internal Medicine (SZTE / ASZMS / DIMedicine); Brunner, Jürgen; Dumfarth, Alexandra; Cejka, Daniel; Flaschberger, Stefan; Flögelova, Hana; Haris, Ágnes [Haris, Ágnes (nefrológia), author] Peterfy Hospital and Jeno Manninger National In...; Hartmann, Ágnes [Hartmann, Ágnes (Gyermekgyógyászat), author] Department of Paediatrics (UP / UPMS); Heilos, Andreas; Mueller, Thomas; Rusai, Krisztina; Arbeiter, Klaus; Hofer, Johannes; Jakab, Dániel [Jakab, Dániel (gyermekgyógyász, ...), author] Department of Paediatrics and Paediatric Health... (SZTE / ASZMS); Sinkó, Mária; Szigeti, Erika [Szigeti, Erika (Gyermek gasztroen...), author] Department of Paediatrics and Paediatric Health... (SZTE / ASZMS); Bereczki, Csaba [Bereczki, Csaba (gyermekgyógyászat...), author] Department of Paediatrics and Paediatric Health... (SZTE / ASZMS); Janko, Viktor; Kelen, Kata [Kelen, Kata (gyermekgyógyászat), author] I. Department of Pediatrics (SU / FM / C); Reusz, György S [Reusz, György (Nephrológia), author] I. Department of Pediatrics (SU / FM / C); Szabó, Attila J [Szabó, Attila (Gyermekgyógyászat...), author] I. Department of Pediatrics (SU / FM / C); Klenk, Nóra; Kóbor, Krisztina; Kojc, Nika; Knechtelsdorfer, Maarten; Laganovic, Mario; Lungu, Adrian Catalin; Meglic, Anamarija; Rus, Rina; Kersnik Levart, Tanja; Macioniene, Ernesta; Miglinas, Marius; Pawłowska, Anna; Stompór, Tomasz; Podracka, Ludmila; Rudnicki, Michael; Mayer, Gert; Rysava, Romana; Reiterova, Jana; Saraga, Marijan; Seeman, Tomáš; Zieg, Jakub; Sládková, Eva; Stajic, Natasa; Szabó, Tamás [Szabó, Tamás (Orvostudomány), author] Department of Pediatrics (UD); Capitanescu, Andrei; Stancu, Simona; Tisljar, Miroslav; Galesic, Kresimir; Tislér, András [Tislér, András (belgyógyászat, ne...), author] Department of Internal Medicine and Oncology (SU / FM / C); Vainumäe, Inga; Windpessl, Martin; Zaoral, Tomas; Zlatanova, Galia; Józsi, Mihály** [Józsi, Mihály (Immunológia), author] Department of Immunology (ELTE / ELU FoS / Bio_I); MTA-ELTE Complement Research Group (ELTE / ELU FoS / Bio_I / Dept_Immun); Csuka, Dorottya [Csuka, Dorottya (Humán molekuláris...), author] Belgyógyászati és Hematológiai Klinika (SU / FM / C)

English Study Group (Journal Article) Scientific
Published: FRONTIERS IN IMMUNOLOGY 1664-3224 1664-3224 12 Paper: 720183 , 20 p. 2021
  • SJR Scopus - Immunology: Q1
Identifiers
Fundings:
  • (PPD2018-016/2018)
  • (2020-1.1.6-JOVO-2021-00013)
  • (EFOP-3.6.3-VEKOP16-2017-00009)
  • (0106307) Funder: MTA
  • (K125219) Funder: HSRF
  • (D11206)
  • (NKFIH-1157/8/2019)
Subjects:
  • Immunology
  • Clinical medicine
  • Nephrology
Factor H-related protein 5 (FHR-5) is a member of the complement Factor H protein family. Due to the homology to Factor H, the main complement regulator of the alternative pathway, it may also be implicated in the pathomechanism of kidney diseases where Factor H and alternative pathway dysregulation play a role. Here, we report the first observational study on CFHR5 variations along with serum FHR-5 levels in immune complex-mediated membranoproliferative glomerulonephritis (IC-MPGN) and C3 glomerulopathy (C3G) patients together with the clinical, genetic, complement, and follow-up data.A total of 120 patients with a histologically proven diagnosis of IC-MPGN/C3G were enrolled in the study. FHR-5 serum levels were measured in ELISA, the CFHR5 gene was analyzed by Sanger sequencing, and selected variants were studied as recombinant proteins in ELISA and surface plasmon resonance (SPR).Eight exonic CFHR5 variations in 14 patients (12.6%) were observed. Serum FHR-5 levels were lower in patients compared to controls. Low serum FHR-5 concentration at presentation associated with better renal survival during the follow-up period; furthermore, it showed clear association with signs of complement overactivation and clinically meaningful clusters.Our observations raise the possibility that the FHR-5 protein plays a fine-tuning role in the pathogenesis of IC-MPGN/C3G.
Cited in (16)
Citing (10)
Citation styles: IEEEACMAPAChicagoHarvardCSLCopyPrint
2025-04-26 09:29