https://m2.mtmt.hu/ eng 2026-10-24 12:54 JournalArticle 32237399 VALIDATED true 0 false 2026-10-21T18:10:04.740+0000 2022-10-11T08:42:55.392+0000 2021-09-24T08:39:44.471+0000 true 10019782 Csámer Iván /api/admin/10019782 Admin Iván Csámer (ELTE TTK kari admin 5) true 10019782 2026-03-25T17:14:04.553+0000 2026-03-25T17:14:04.553+0000 2022-10-11T08:42:55.676+0000 true 10062062 Gere Tamásné /api/admin/10062062 Admin Tamásné Gere (SE_KK_Admin5_GT, admin) true 10062062 Admin 10065569 /api/admin/10065569 Éva Pécsi (MTMT Közp 2,3, admin) Pécsi Éva true true 2026-04-14T13:01:34.757+0000 true false true 24 24 /api/publicationtype/24 PublicationType Journal Article 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType Journal Article 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType Article (Journal Article) 101 true 10000059 true 1 /api/category/1 Category Scientific true 1 Nemeth, Krisztina true true /api/journal/10012860 REVIEWED ANALYTICAL METHODS: ADVANCING METHODS AND APPLICATIONS 1759-9660 1759-9679 true false 10012860 false 10012860 true 1759-9660 1759-9679 Journal FOREIGN 13 39 4614 4622 4614 9 2021 Global DNA methylation and hydroxymethylation play an important role in gene expression. They can be connected with several diseases. The modification status could be a biomarker to determine the status of disease. A fast, easy and accurate liquid chromatography - tandem mass spectrometry method has been developed for the precise quantitation of 5-methylcytosine and 5-hydroxymethylcytosine. Formic acid was used for the hydrolysis of the DNA strand resulting in nucleobases. These polar hydrolysis products were separated on a normal phase column using reversed phase eluents in inverse gradient mode. Multiple reaction monitoring was applied to achieve high selectivity and sensitivity for the quantitation. A new relative quantitation model was developed by using guanine, as an internal standard, present in samples. The new method was successfully validated with excellent accuracy and precision values in the range of 0.005-0.5% for 5hmC and 1-15% for 5mC. The main advantages of this quantitation method are that, due to relative quantitation, calibration curves can be used without reacquiring the calibration points and no additional isotope labeled internal standards are required. The method was tested to identify the concentrations of 5mC and 5hmC in various sample types. The lowest level of DNA sample required in the case of 0.005% 5hmC is 0.5 mu g. Funding 2008372 /api/funding/2008372 Higher Education Institutional Excellence Programme of the Ministry of Human Capacities in Hungary(20428-3/2018/FEKUTSTRAT) Funder: Ministry of Human Capacities false true Funding 2008373 /api/funding/2008373 (K_132695) false true Funding 2008374 /api/funding/2008374 (NKFI FK 135065) Funder: NRDIO false true true 2021 true true true false true false GREEN 2026-10-21 true https://edit.elte.hu/handle/10831/110491 8 0 8 5 3 8 8 8 8 8 8 5 5 5 3 0 8 5 8 5 0 3 3 Folyóiratcikk Journal Article 24 8 Könyvrészlet Chapter in Book 25 0 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2022 0 1 0 1 0 2023 0 1 0 1 0 2024 0 2 2 2 2 2025 0 3 2 3 2 2026 0 1 1 1 1 Q2 false 10014589,10076057 false false false 2021-11-02T09:29:49.577+0000 Enzimológiai Intézet (TTK); Hevesy György Kémia Doktori Iskola (ELTE / TTK); Laboratóriumi Medicina Intézet (SE / AOK / I); MTA-SE Örökletes Daganatok Kutatócsoport (SE / AOK / I / LABMEDINT); Molekuláris Biológiai Tanszék (SE / AOK / I / BMBI) 6 40 5 6 0 true 10065569 Language 10002 /api/language/10002 English Angol English true 2 true PersonAuthorship 97629827 /api/authorship/97629827 Nemeth, Krisztina [Németh, Krisztina (Tömegspektrometria), author] Hevesy György PhD School of Chemistry (ELTE / ELU FoS) 1 0.167 true false false Author 10076057 /api/author/10076057 Krisztina Németh (Tömegspektrometria) Németh Krisztina true true Nemeth Krisztina true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 97629828 /api/authorship/97629828 Meszaros, Katalin [Mészáros, Katalin (orvosi laboratóri...), author] MTA-SE Research Group for Hereditary Tumors (SU / FM / I / DLM) 2 0.16666667 false false false Author 10058121 /api/author/10058121 Katalin Mészáros (orvosi laboratóriumi diagnosztika) Mészáros Katalin true 10058121 true Meszaros Katalin true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 97629829 /api/authorship/97629829 Szabo, Borbala [Szabó, Borbála (Endokrinológia), author] Department of Laboratory Medicine (SU / FM / I) 3 0.16666667 false false false Author 10065387 /api/author/10065387 Borbála Szabó (Endokrinológia) Szabó Borbála true true Szabo Borbala true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 97629830 /api/authorship/97629830 Butz, Henriett [Butz, Henriett (orvostudomány), author] Department of Laboratory Medicine (SU / FM / I); MTA-SE Research Group for Hereditary Tumors (SU / FM / I / DLM) 4 0.167 false false false Author 10020420 /api/author/10020420 Henriett Butz (orvostudomány) Butz Henriett true 10020420 true Butz Henriett true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 97629831 /api/authorship/97629831 Aranyi, Tamas [Arányi, Tamás (Génexpresszió sza...), author] Institute of Enzymology (RCNS); Molekuláris Biológiai Tanszék (SU / FM / I / BMBI) 5 0.16666667 false false false Author 10012617 /api/author/10012617 Tamás Arányi (Génexpresszió szabályozás) Arányi Tamás true 10012617 true Aranyi Tamas true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 97629832 /api/authorship/97629832 Szabo, Pal T. ✉ [Szabó, Pál Tamás (Tömegspektrometria), author] 6 0.167 false true true Author 10014589 /api/author/10014589 Pál Tamás Szabó (Tömegspektrometria) Szabó Pál Tamás true 10014589 true Szabo Pal T. true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PublicationIdentifier 19385514 /api/publicationidentifier/19385514 DOI: 10.1039/d1ay00897h PlainSource 6 /api/publicationsource/6 DOI PublicationSourceType 10001 /api/publicationsourcetype/10001 DOI true true true DOI DOI https://doi.org/@@@ true true 6 true IDENTICAL 10.1039/d1ay00897h https://doi.org/10.1039/d1ay00897h false true PublicationIdentifier 19385513 /api/publicationidentifier/19385513 WoS: 000696275500001 PlainSource 1 /api/publicationsource/1 WoS PublicationSourceType 10003 /api/publicationsourcetype/10003 Indexelő adatbázis false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true IDENTICAL 000696275500001 https://www.webofscience.com/wos/woscc/full-record/000696275500001 false true PublicationIdentifier 26854937 /api/publicationidentifier/26854937 EDIT: 110491 SwordSource 92 /api/publicationsource/92 EDIT PublicationSourceType 10007 /api/publicationsourcetype/10007 Repozitórium true true true EDIT EDIT https://edit.elte.hu/handle/10831/@@@ true true 92 true GREEN true 110491 https://edit.elte.hu/handle/10831/110491 false true PublicationIdentifier 20140265 /api/publicationidentifier/20140265 Scopus: 85117526674 PlainSource 3 /api/publicationsource/3 Scopus PublicationSourceType 10003 /api/publicationsourcetype/10003 Indexelő adatbázis false true true Scopus Scopus http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@ true true 3 true IDENTICAL 85117526674 http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85117526674 true true PublicationIdentifier 19747299 /api/publicationidentifier/19747299 PubMed: 34528637 PlainSource 17 /api/publicationsource/17 PubMed PublicationSourceType 10003 /api/publicationsourcetype/10003 Indexelő adatbázis false true true PubMed PubMed http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=@@@&dopt=Abstract true true 17 true IDENTICAL 34528637 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=34528637&dopt=Abstract true true Classification 12713 /api/classification/12713 Other agricultural sciences true true Classification 11082 /api/classification/11082 Other engineering and technologies true true Classification 10293 /api/classification/10293 Chemical sciences true true SjrRating 11203272 /api/sjrrating/11203272 sjr:Q2 (2021) Scopus - Analytical Chemistry ANALYTICAL METHODS: ADVANCING METHODS AND APPLICATIONS 1759-9660 1759-9679 53 0.49 journal RatingType 10002 /api/ratingtype/10002 sjr sjr true true ClassificationExternal 1602 /api/classificationexternal/1602 Scopus - Analytical Chemistry true 1602 true Q2 DIRECT true true Reference 23569176 /api/reference/23569176 1. Szabo, Borbala: Demethylation Status of Somatic DNA Extracted From Pituitary Neuroendocrine Tumors Indicates Proliferative Behavior; 1 false true Reference 23569177 /api/reference/23569177 2. Rastegar, Leila: A comparison and column selection of Hydrophilic Interaction Liquid Chromatography and Reversed-Phase High-Performance Liquid Chromatography for detection of DNA methylation; 2 false true Reference 23569178 /api/reference/23569178 3. Jiang, Tingyun: Variation in global DNA hydroxymethylation with age associated with schizophrenia; 3 false true Reference 23569179 /api/reference/23569179 4. Ye, Xiaoxia: A quantitative method for detecting DNA methylation over targeted genomic regions using isotope dilution liquid chromatography tandem mass spectrometry; 4 false true Reference 23569180 /api/reference/23569180 5. Jeschke, Jana: Portraits of TET-mediated DNA hydroxymethylation in cancer; 5 false true Reference 23569181 /api/reference/23569181 6. Schuebeler, Dirk: Function and information content of DNA methylation; 6 false true Reference 23569182 /api/reference/23569182 7. Shukla, Ankita: Hydroxymethylation and its potential implication in DNA repair system: A review and future perspectives; 7 false true Reference 23569183 /api/reference/23569183 8. Taleat, Zahra: Detection strategies for methylated and hypermethylated DNA; 8 false true Reference 23569184 /api/reference/23569184 9. Putra, Sulistyo Emantoko Dwi: Analysis of Genomic DNA Methylation Levels in Human Placenta using Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry; 9 false true Reference 23569185 /api/reference/23569185 10. Kohli, Rahul M.: TET enzymes, TDG and the dynamics of DNA demethylation; 10 false true Reference 23569186 /api/reference/23569186 11. Jursch, Tobias: Regulation of DNA transposition by CpG methylation and chromatin structure in human cells; 11 false true Reference 23569187 /api/reference/23569187 12. Grayson, Dennis R.: The Dynamics of DNA Methylation in Schizophrenia and Related Psychiatric Disorders; 12 false true Reference 23569188 /api/reference/23569188 13. Tang, Yang: Widespread Existence of Cytosine Methylation in Yeast DNA Measured by Gas Chromatography/Mass Spectrometry; 13 false true Reference 23569189 /api/reference/23569189 14. Zhang, Liangtao: Simultaneous Determination of Global DNA Methylation and Hydroxymethylation Levels by Hydrophilic Interaction Liquid Chromatography-Tandem Mass Spectrometry; 14 false true Reference 23569190 /api/reference/23569190 15. Yamagata, Yoshiaki: Rapid turnover of DNA methylation in human cells; 15 false true Reference 23569191 /api/reference/23569191 16. Wu, Hao: Mechanisms and functions of Tet protein-mediated 5-methylcytosine oxidation; 16 false true Reference 23569192 /api/reference/23569192 17. Li, Xingnan: High-throughput and cost-effective global DNA methylation assay by liquid chromatography-mass spectrometry; 17 false true Reference 23569193 /api/reference/23569193 18. Wang, Xiaoli: Ultra-performance liquid chromatography/tandem mass spectrometry for accurate quantification of global DNA methylation in human sperms; 18 false true Reference 23569194 /api/reference/23569194 19. Rocha, Monica S.: Global DNA methylation: comparison of enzymatic- and non-enzymatic-based methods; 19 false true Reference 23569195 /api/reference/23569195 20. Zhang, Jun-jie: Analysis of global DNA methylation by hydrophilic interaction ultra high-pressure liquid chromatography tandem mass spectrometry; 20 false true Reference 23569196 /api/reference/23569196 21. Thuc Le: A sensitive mass spectrometry method for simultaneous quantification of DNA methylation and hydroxymethylation levels in biological samples; 21 false true Reference 23569197 /api/reference/23569197 22. Jin, Seung-Gi: Examination of the specificity of DNA methylation profiling techniques towards 5-methylcytosine and 5-hydroxymethylcytosine; 22 false true Reference 23569198 /api/reference/23569198 23. Liu, Zhongfa: Quantification of regional DNA methylation by liquid chromatography/tandem mass spectrometry; 23 false true Reference 23569199 /api/reference/23569199 24. Straussman, Ravid: Developmental programming of CpG island methylation profiles in the human genome; 24 false true Reference 23569200 /api/reference/23569200 25. Shi, Huidong: CpG islands: their potential as biomarkers for cancer; 25 false true Reference 23569201 /api/reference/23569201 26. Kok, Robert M.: Global DNA methylation measured by liquid chromatography-tandem mass spectrometry: analytical technique, reference values and determinants in healthy subjects; 26 false true Reference 23569202 /api/reference/23569202 27. Baylin, SB: Epigenetic gene silencing in cancer - a mechanism for early oncogenic pathway addiction?; 27 false true /api/publication/32237399 Nemeth Krisztina et al. A relative quantitation method for measuring DNA methylation and hydroxymethylation using guanine as an internal standard. (2021) ANALYTICAL METHODS: ADVANCING METHODS AND APPLICATIONS 1759-9660 1759-9679 13 39 4614-4622<div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10076057"><a href="/gui2/?type=authors&mode=browse&sel=10076057" target="_blank">Nemeth Krisztina (<span class="authorship-author-name">Németh Krisztina</span> <span class="authorAux-mtmt"> Tömegspektrometria</span>) </a> </span> <span class="author-affil"><span title="Eötvös Loránd University">ELTE</span>/<span title="Faculty of Science">ELU FoS</span>/Hevesy György PhD School of Chemistry</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10058121"><a href="/gui2/?type=authors&mode=browse&sel=10058121" target="_blank">Meszaros Katalin (<span class="authorship-author-name">Mészáros Katalin</span> <span class="authorAux-mtmt"> orvosi laboratóriumi diagnosztika</span>) </a> </span> <span class="author-affil"><span title="Semmelweis University">SU</span>/<span title="Faculty of Medicine">FM</span>/<span title="Instituties">I</span>/<span title="Department of Laboratory Medicine">DLM</span>/MTA-SE Research Group for Hereditary Tumors</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10065387"><a href="/gui2/?type=authors&mode=browse&sel=10065387" target="_blank">Szabo Borbala (<span class="authorship-author-name">Szabó Borbála</span> <span class="authorAux-mtmt"> Endokrinológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis University">SU</span>/<span title="Faculty of Medicine">FM</span>/<span title="Instituties">I</span>/Department of Laboratory Medicine</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10020420"><a href="/gui2/?type=authors&mode=browse&sel=10020420" target="_blank">Butz Henriett (<span class="authorship-author-name">Butz Henriett</span> <span class="authorAux-mtmt"> orvostudomány</span>) </a> </span> <span class="author-affil"><span title="Semmelweis University">SU</span>/<span title="Faculty of Medicine">FM</span>/<span title="Instituties">I</span>/Department of Laboratory Medicine; <span title="Semmelweis University">SU</span>/<span title="Faculty of Medicine">FM</span>/<span title="Instituties">I</span>/<span title="Department of Laboratory Medicine">DLM</span>/MTA-SE Research Group for Hereditary Tumors</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10012617"><a href="/gui2/?type=authors&mode=browse&sel=10012617" target="_blank">Aranyi Tamas (<span class="authorship-author-name">Arányi Tamás</span> <span class="authorAux-mtmt"> Génexpresszió szabályozás</span>) </a> </span> <span class="author-affil"><span title="Research Centre for Natural Sciences">RCNS</span>/Institute of Enzymology; <span title="Semmelweis University">SU</span>/<span title="Faculty of Medicine">FM</span>/<span title="Instituties">I</span>/<span title="Biokémiai és Molekuláris Biológiai Intézet">BMBI</span>/Molekuláris Biológiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10014589"><a href="/gui2/?type=authors&mode=browse&sel=10014589" target="_blank">Szabo Pal T. ✉ (<span class="authorship-author-name">Szabó Pál Tamás</span> <span class="authorAux-mtmt"> Tömegspektrometria</span>) </a> </span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;32237399" target="_blank">A relative quantitation method for measuring DNA methylation and hydroxymethylation using guanine as an internal standard</a></div> <div> <span class="journal-title">ANALYTICAL METHODS: ADVANCING METHODS AND APPLICATIONS</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/1759-9660">1759-9660</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1759-9679">1759-9679</a>)</span>: <span class="journal-volume">13</span> <span class="journal-issue">39</span> <span class="page"> pp 4614-4622 </span> <span class="year">(2021)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Language: English | </span> <span class="identifiers"> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="10.1039/d1ay00897h" target="_blank" href="https://doi.org/10.1039/d1ay00897h"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000696275500001" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000696275500001"> WoS </a> </span> <span class="id identifier oa_GREEN" title=" Green "> <a style="color:black" title="110491" target="_blank" href="https://edit.elte.hu/handle/10831/110491"> EDIT </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="85117526674" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85117526674"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="34528637" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=34528637&dopt=Abstract"> PubMed </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Journal subject: Scopus - Analytical Chemistry&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q2</div> <div class="journal-subject">Journal subject: Scopus - Chemical Engineering (miscellaneous)&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q2</div> <div class="journal-subject">Journal subject: Scopus - Engineering (miscellaneous)&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q2</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="" class="citingPub-count">Citing papers: 8</span> | Independent citation: 5 | Self citation: 3 | Unknown citation: 0 | Number of citations in WoS: 8 | Number of citations in Scopus:&nbsp;8 | WoS/Scopus assigned:&nbsp;8 | Number of citations with DOI:&nbsp;8 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;32237399&sort=publishedYear,desc&sort=title"> Number of cited publications: 3 </a> </div> <div class="mtid"><span class="long-pub-mtid">Publication: 32237399</span> | <span class="status-data status-VALIDATED"> Validated </span> Core Citing | <span class="type-subtype">Journal Article ( Article ) </span> | <span class="pub-category">Scientific</span> | <span class="publication-sourceOfData">kézi felvitel</span> </div> <div class="funder"> Higher Education Institutional Excellence Programme of the Ministry of Human Capacities in Hungary(20428-3/2018/FEKUTSTRAT) Funder: Ministry of Human Capacities, (K_132695), (NKFI FK 135065) Funder: NRDIO </div> <div class="lastModified">Last Modified: 2022.10.11. 10:42 Tamásné Gere (SE_KK_Admin5_GT, admin) </div> <div class="lockedBy">Centrally managed 2026.04.14. 15:01 Éva Pécsi (MTMT Közp 2,3, admin) </div> </div></div>